| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
We have already developed micro-arrayed chemical sensors which consist of optical sensor film for pH or oxygen, and micro well array prepared with PDMS, for parallel monitoring of single cell activity. In this study, 10 gm+ pH and oxygen sensor spots were arranged by using microcontact printing technology.
PDMS stamp was replicated from a mold prepared with SU-8. The prepared stamp (spot: 10 um i.d., pitch: 40 pm) was set to the micro contact printer. FITC and dichlorotris(1,10-phenanthroline) ruthenium (II) were used as pH and oxygen sensor reagent respectively. These sensor reagents were stamped onto a diamond like carbon sputtered slide glass. Fluorescence intensity of each spot was measured by using a laser conforcal high resolution micro-array scanner.
For microcontact printing of pH sensor spots, sensor reagent was infiltrated into a lens cleaning tissue and it was used as an ink pad. Averaged spot size was 10.0+0.5 μm (n=48). Response to 0.05M phosphate buffer of pH 6-8 were measured. Averaged fluorescence intensity of 100 spots normalized with the value at pH 7 showed good correlation with pH. For microcontact printing of oxygen sensor spots, sensor reagent was dropped onto a PDMS stamp, and was developed by using an air gun. Averaged spot size was 9.9+0.6 μm(n=25). Sensor response was evaluated by using distilled water aerated with oxygen or nitrogen, and 5% sodium sulfite solution. Averaged fluorescence intensity of 99 spots normalized with the value at normal distilled water showed good correlation with oxygen concentration.
Finally, after the printing of oxygen sensor spots, pH sensor spots were stamped between oxygen sensor spots by using the same PDMS stamp. The response of the prepared integrated pH and oxygen sensor array was measured by using a micro array scanner. Each sensor spot showed specific responses.
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