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Fabrication of Nanoparticles by Gamma-ray crosslinking of thermosensitive polypeptides and analysis for controled release of drugs

Research Project

Project/Area Number 18560803
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nuclear engineering
Research InstitutionOsaka Prefecture University

Principal Investigator

FURUTA Masakazu  Osaka Prefecture University, Graduate School of Sciences, Associate Professor (40181458)

Co-Investigator(Kenkyū-buntansha) OKUDA Shuichi  Osaka Prefecture University, Organization of Universtity-Industry-Govemment Cooporation, Professor (00142175)
OASAKA Masayuki  Oasaka Prefecture Universty, Graduate School of Sciences, Professor (50344172)
OKAMOTO Kouji  Kyushu Institute of Technology, Department of Bioscience and Bioinformatics, Professor (40122618)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsNano materials / Thermosensitivity / Polypeptide / Radiation Crosslinking
Research Abstract

Elastin is an essential component in animal tissues as well as collagen. Elastin molecules are formed by covalent bounds of a cross-linked network and their network can stretch and recoil like rubber band. This elasticity conform a flexibility to such as skin, blood vessels, and lungs, need to this elasticity in order to function [6]. Elastin molecules are composed largely of hydrophobic amino acid residues, such as glycine (G) ,alanine (A) ,proline (P) and valine (V) and their amino acids repeat as (GVGVP)n exist in the animals. On raising the temperature, they folded and assembled above a specific temperature called cloud point (CP) and it formed helical structure of type II beta-turn. Using these hydrophobic amino acid residues, unique thermoresponsive polypeptides: poly [(GVGVP)_<251>]was synthesized by Urry, et al. using Escherichia coli recombinant DNA technology and these polypeptides exhibit a remarkable biocompatibility. Utilizing these thermosensitive polypeptides, Nanoparticles were prepared utilizing the thermosensitive aggregation of the elastin model polypeptides; poly[(GVGVP)_<251>]and gamma-ray crosslinking. Three different heating methods, "Slow heating", "Fast heating", and "Heat shock", were applied for the aggregation of the peptide and examined to yield stable nanoparticles by gamma-ray crosslinking. As a result, only "heat shock" procedure yielded stable nanoparticles with diameters of less than ca. 150 nm and a narrow size distribution. CD spectrometry showed that this polypeptide formed type II beta-turn structure on raising temperature above cloud point(CP)with the "heat shock" procedure and, kept the structure within the crosslinked nanoparticles, suggesting that this structure would be crucial to yield stable crosslinked particles.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (46 results)

All 2008 2007 2006

All Journal Article (19 results) (of which Peer Reviewed: 4 results) Presentation (25 results) Book (2 results)

  • [Journal Article] Coacervation and function:Fabrication of nanoparticles and application to DDS(in Japanese)2008

    • Author(s)
      M. Furuta
    • Journal Title

      Elastin-Structure,Function,Pathology-,H.Ito Ed.Japan Elastin Research Association

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH condition2007

    • Author(s)
      N.Inoue
    • Journal Title

      Journal of Biomater.Sci.Polymer Edn 17(8)

      Pages: 837-858

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Solution Property and Irradiation Effect of Random Copolypeptides Composed of Ala and Pro Residues2007

    • Author(s)
      S.Kakinoki
    • Journal Title

      Polymer Bulletin 58

      Pages: 393-400

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Domains 16 and 17 of tropoelastin in elastic fibre formation2007

    • Author(s)
      H.Wachi
    • Journal Title

      Biochem.J 402

      Pages: 63-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Induction of macrophage migration throug hlactose-insensitive receptor by elastin-derived nonapeptide and their analog2007

    • Author(s)
      I.Maeda
    • Journal Title

      Biochem.J 13

      Pages: 263-268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Immunochoemical and immunohistochemical studies on distribution of elastin fibres in human atherosclerotic hexapeptide repeat2007

    • Author(s)
      I.Maeda
    • Journal Title

      Biochem.J 142

      Pages: 263-268

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Solution Property and Irradiation Effect of Random Copolypeptides Composed of Ala and Pro Residues(Refereed)2007

    • Author(s)
      S. Kakinoki, et. al.
    • Journal Title

      Polymer Bulletin 58

      Pages: 393-400

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Domains 16 and 17 of tropoelastin in elastic fibre formation(Refereed)2007

    • Author(s)
      H. Wachi, et. al.
    • Journal Title

      Biochem.J 402

      Pages: 63-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Induction of macrophage migration through lactose-insensitive receptor by elastin-derived nonapeptide and their analog(Refereed)2007

    • Author(s)
      I. Maeda, et. al.
    • Journal Title

      J.Peptide Soil 13

      Pages: 263-268

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Immunochoemical and immunohistochemical studies on distribution of elastin fibres in human atherosclerotic hexapeptide repeat(Refereed)2007

    • Author(s)
      I. Maeda, et. al.
    • Journal Title

      J.Biochem 142

      Pages: 627-631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nanoparticles from elastin-based polymers as drug release devices(Non-refereed)Book2007

    • Author(s)
      K. Okamoto, et. al.
    • Journal Title

      Biopolymers 88(4)

      Pages: 618-618

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Solution Property and Irradiation Effect of Random Copolypeptides Composed of Ala and Pro Residues2007

    • Author(s)
      S.Kakinokil
    • Journal Title

      Polymer Bulletin 58

      Pages: 393-400

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nanoparticles preparation using elastin-derived polypeptide and co-polypeptide for design of drug release devices2006

    • Author(s)
      A.Doi
    • Journal Title

      Peptide Science 2005

      Pages: 281-282

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nanoparticles from elastin-based polymers as drug release devices2006

    • Author(s)
      K.Okamoto
    • Journal Title

      J.Peptide Sci 12

      Pages: 243-243

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nanoparticles from elastin-based polymers as drug release devices2006

    • Author(s)
      K.Okamoto
    • Journal Title

      Biopolymers 88(4)

      Pages: 618-618

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH condition,(Refereed)2006

    • Author(s)
      N. Inoue, et. al.
    • Journal Title

      J.Biomater.Sol.Polymer Edn 17(8)

      Pages: 837-858

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nanoparticles preparation using elastin-derived polypeptide and co-polypeptide for design of drug release devices(Non-refereed)2006

    • Author(s)
      A. Doi, et. al.
    • Journal Title

      Peptide Science 2005

      Pages: 281-282

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nanoparticles from elastin-based polymers as drug release devices(Non-refereed)2006

    • Author(s)
      K. Okamoto, et. al.
    • Journal Title

      J.Peptide Sui 12

      Pages: 243-243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH condition2006

    • Author(s)
      N.Inoue, M.Bessho, M.Furuta, T.kojima, S.Okuda, M.Hara
    • Journal Title

      Journal of biomater.Sci.Polymer Edn. 17(8)

      Pages: 837-858

    • Related Report
      2006 Annual Research Report
  • [Presentation] Fabrication of Nano-particles by Gamma-ray Crosslinking of Elastic Polypeptides and application to DDS,2008

    • Author(s)
      Masakazu Furuta
    • Organizer
      The 3rd International 21^<st> Century COE Symposium on Science and Engineering for Evolution of Valuable Resources and Energy from Organic Wastes
    • Place of Presentation
      Osaka Prefecture University, Sakai, Osaka, Japan.
    • Year and Date
      2008-03-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] Fabrication of nano-particles by gamma-raycross linking of elastic polypeptides and application to DDS2008

    • Author(s)
      M.Fujimoto
    • Organizer
      The 3rd International 21st Century COE Symposium on Science and Engeneering for Evolution of Valuable Resources and Energy from Organic Wastes
    • Place of Presentation
      大阪府立大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fabrication of Nano-particles by Gamma-ray Crosslinking of Elastic Polypeptides a nd application to DDS2008

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      The 3rd International 21st Century COE Symposium on Science and Engineering for Evolution of Valuable Resources and Energy from Organic Wastes
    • Place of Presentation
      Osaka Prefecture University, Sakai, Osaka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温度感受性エラスチンモデルペプチドのガンマ線架橋によるナノ粒子化とその応用2007

    • Author(s)
      藤本 真理
    • Organizer
      第29回日本バイオマテリアル学会
    • Place of Presentation
      千里ライフサイエンスセンタ
    • Year and Date
      2007-11-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温度感受性エラスチンモデルペプチドのガンマ線架橋によるナノ粒子化とその応用2007

    • Author(s)
      藤本 真理
    • Organizer
      第29回日本バイオマテリアル学会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2007-11-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] ガンマ線架橋によるエラスチンモデルペプチドのナノ微粒子化2007

    • Author(s)
      藤本 真理
    • Organizer
      第59回日本生物工学会大会
    • Place of Presentation
      広島大学東広島キャンパス総合科学部
    • Year and Date
      2007-09-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 放射線を利用した温度感受性エラスチンモデルペプチドのナノ粒子化とDDSとしでの応用2007

    • Author(s)
      藤本 真理
    • Organizer
      日本バイオマテリアル学会第2回関西若手研究発表会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2007-08-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fabrication of thermosensitive elastic polypeptide nano-particles by gamma-irradiation and application to DDS2007

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      The 2nd Western Regional Young Scientists'Meeting by Japanese Society for Biomaterials
    • Place of Presentation
      Osaka Prefecture University
    • Year and Date
      2007-08-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 放射線を利用した温度感受性エラスチンモデルペプチドのナノ粒子化とDDSとしての応用2007

    • Author(s)
      藤本 真理
    • Organizer
      日本バイオマテリアル学会第2回関西若手研究発表会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2007-08-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] エラスチンモデルペプチドの放射線架橋によりナノ微粒子を作る2007

    • Author(s)
      古田 雅一
    • Organizer
      第6回国際バイオフォーラム、大学・国公立研究所による研究成果発表フォーラム
    • Place of Presentation
      東京ビッグサイト
    • Year and Date
      2007-06-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Fabrication of Elastic Polypeptide Nano-particles by gamma-irradiation and application to DDS2007

    • Author(s)
      M.Fujimoto
    • Organizer
      Th・2nd International 12st Century COE Syposium on Science and Engineering for Evolution of Valuable Resources and Energy from Organic Wastes
    • Place of Presentation
      大阪府立大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fabrication of elastic polypeptide nano-particles by gamma-irradiation and application to DDS2007

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      The 2ed International 21st Century COE Symposium on Science and Engineering for Evolution of Valuable Resources and Energy from Organic Wastes
    • Place of Presentation
      Osaka Prefecture University, Sakai, Osaka, JAPAN
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of nanoparticles by radiation crosslinking of elastin model polypeptide2007

    • Author(s)
      M. Furuta, et. al.
    • Organizer
      Research achievement presentation forum,-BioExpo JAPAN-
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Nano-particle formation by gamma-crosslinking of thermosensitive elastic polypeptides2007

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      The The Society for Biotechnology,Japan/59th Annual Meeting
    • Place of Presentation
      Hiroshima Universtiy
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Nano-particle formation by gamma-crosslinking of thermosensitive elastic polypeptides and their application2007

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      The 29th Annal Meeting of Japanese Society for Biomaterials
    • Place of Presentation
      Senri Life Science Center, Suita, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of Glu-cntaining elastic polypeptide nano-particles by gamma-irradiation for applicationto DDS2006

    • Author(s)
      M.Furuta
    • Organizer
      Particle 2006
    • Place of Presentation
      Wyndham Orlando Resort, 8001 International Drive Orlando Florida, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Drug Release from Nano-particles Made of Radiation Cross linked Elastic Model Polypeptides2006

    • Author(s)
      M.Furuta
    • Organizer
      Particle 2006
    • Place of Presentation
      Wyndham Orlando Resort, 8001 International Drive Orlando Florida, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DDSを目的としたエラスチンモデルペプチドの放射線架橋およびナノ粒子化の検討2006

    • Author(s)
      古田 雅一
    • Organizer
      第9回日本組織工学会
    • Place of Presentation
      京都テルサ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温度感受性エラスチンモデルペプチドの放射線架橋によるナノ粒子開発2006

    • Author(s)
      古田 雅一
    • Organizer
      日本原子力学会2006秋の大会
    • Place of Presentation
      北海道大学工学部
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fabrication of Elastic Polypeptide Nano-particles by gamma-irradiation and application to DDS2006

    • Author(s)
      M.Fujimoto
    • Organizer
      Nanobio-Tokyo, 2006
    • Place of Presentation
      東京大学本郷キャンパス
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chraracterization of Glu-containing elastic polypeptide nano-particles by gamma-irradiation for application to DDS2006

    • Author(s)
      M. Furuta, et. al.
    • Organizer
      Particle2006
    • Place of Presentation
      Orlando, Florida, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Drug release from nano-particles made of radiation crosslinked elastic model polypeptides2006

    • Author(s)
      M. Furuta, et. al.
    • Organizer
      Particle2006
    • Place of Presentation
      Orlando, Florida, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Examination of radiation crosslinking to obtain nanoparticles of elastin model polypeptide for DDS2006

    • Author(s)
      M. Furuta, et. al.
    • Organizer
      The 9th Annual Meetin of the Japanese Society for Tissue Engineering
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of nanoparticles utilizing radiation crosslinking of thermosensitive elastin model polypeptide2006

    • Author(s)
      M. Furuta, et. al.
    • Organizer
      2006 Fall Meeting of AESJ
    • Place of Presentation
      Hokkaido University
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fabrication of elastic polypeptide nano-particles by gamma-irradiation and application to DDS2006

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      Nanobio-Tokyo
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] エラスチン-構造・機能・病理2007

    • Author(s)
      古田 雅一
    • Total Pages
      299
    • Publisher
      エラスチン研究会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] エラスチン-構造・機能・病理-2007

    • Author(s)
      古田 雅一
    • Total Pages
      299
    • Publisher
      エラスチン研究会
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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