Molecular-genetic study of the circadian clock mechanism using the moss Physcomitrella patens as a model plant

• Project/Area Number: 18570004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Nagoya University
• Principal Investigator: AOKI Setsuyuki Nagoya University, 大学院・情報科学研究科, 講師 (30283469)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: 概日リズム / 時計遺伝子 / 植物の概日時計の進化 / ヒメツリガネゴケ / 概日時計 / 概日時計の進化 / 遺伝子破壊 / 逆遺伝学的解析

Research Abstract

モデル基部植物ヒメツリガネゴケを用いて、被子植物の時計遺伝子のホモログの網羅的な同定・クローニングを行った。それらのうちCCA1/LHYホモログのプロモーターをルシフェラーゼ遺伝子に連結し、コケゲノムに移入し、従来より効果的な発光リズムレポーター株を作出した。このレポーター株をホストとして用いた逆遺伝学的解析により、CCA1/LHYやPRRなどの被子植物の時計遺伝子のホモログの機能を調査し、コケの時計を構成すると思われる遺伝子ネットワークの一部を明らかにし、被子植物の時計機構の分子モデルとの比較を行った。また、コケの色素体シグマ因子遺伝子のひとつPpSIG5の逆遺伝学的解析により、PpSIG5タンパク質がコケ概日システムの出力系の重要な調節因子である可能性を示唆する結果を得た。さらに、コケプロトプラストを用いた、時計遺伝子の日周条件下での発現制御解析のためのトランジェントアッセイ系の開発を行った。

Research Products

• [Journal Article] The genome of the moss Physcomitrella patens reveals evolutionary insights into the conquest of land by plants (2008)
  • Author(s): Stefan A. Rensing, Daniel Lang, Tomoaki Nishiyama, Yasuko Kamisugi, Takako Tanahashi, Keiko Sakakibara, Tomomichi Fujita, Kazuko Oishi, Tadasu Shin-I, Yoko Kuroki, Atsushi Toyoda, Yutaka Suzuki, Shinichi Hashimoto, Kazuo Yamaguchi, Sumio Sugano, Yuji Kohara, Asao Fujiyama, Aldwin Anterola, Setsuyuki Aoki, Neil Ashton, W. Brad Barbazuk, Elizabeth Barker, Jeffrey Bennetzen, Robert Blankenship, Sung Hyun Cho, Susan Dutcher, Mark Estelle, Jeffrey A. Fawcett, Heidrun Gundlach, Kosuke Hanada, Alexander Heyl, Karen A. Hicks, Jon Hughes, Martin Lohr, Klaus Mayer, Alexander Melkozernov, Takashi Murata, David Nelson, Birgit Pils, Michael Prigge, Bernd Reiss, Tanya Renner, Stephane Rombauts, Paul Rushton, Anton Sanderfoot, Gabriele Schween, Shin-Han Shiu, Kurt Stueber, Frederica L. Theodoulou, Hank Tu, Yves Van de Peer, Paul J. Verrier, Elizabeth Waters, Andrew Wood, Lixing Yang, David Cove, Andrew C. Cuming, Mitsuyasu Hasebe , Susan Lucas, Brent D. Mishler, Ralf Reski, Igor Grigoriev, Ralph S. Quatrano, Jeffrey L. Boore
  • Journal Title: Science 319(5859) Pages: 64-69
  • Peer Reviewed
• [Journal Article] The plastid sigma factor SIG5 is involved in the diurnal regulation of the chloroplast gene psbD in the moss Physcomitrella patens (2008)
  • Author(s): Kazuhiro Ichikawa, Akane Shimizu, Ryo Okada, Santosh B. Satbhai, Setsuyuki Aoki
  • Journal Title: FEBS Letters 582(3) Pages: 405-409
  • Peer Reviewed
• [Journal Article] The genome of the moss Physcomitrella patens reveals evolutionary insights into the conquest of land by plants (2008)
  • Author(s): Stefan A. Rensing
  • Journal Title: Science 319 Pages: 64-69
  • Peer Reviewed
• [Journal Article] The plastid sigma factor SIG5 is involved in the diurnal regulation of the chloroplast gene psbD in the moss Physcomitrella patens (2008)
  • Author(s): Kazuhiro Ichikawa
  • Journal Title: FEBS Letters 582 Pages: 405-409
  • Peer Reviewed
• [Journal Article] The plastid sigma factor SIG5 is involved in the diurnal regulation of the chloroplast gene psbD in the moss Physcomitrell a patens. (2008)
  • Author(s): Kazuhiro Ichikawa
  • Journal Title: FEBS Letters 582 Pages: 405-409
  • Peer Reviewed
• [Journal Article] The genome of the moss Physcomitrella patens reveals evolutionary insights into the conquest of land by plants. (2008)
  • Author(s): Stefan A. Rensing
  • Journal Title: Science 319 Pages: 64-69
  • Peer Reviewed
• [Journal Article] A compact multichannel apparatus for automated real-time monitoring of bioluminescence (2007)
  • Author(s): Kazuhisa Okamoto, Masahiro Ishiura, Tatsuharu Torii and Setsuyuki Aoki
  • Journal Title: Journal of Biochemical and Biophysical Methods 70(4) Pages: 535-538
  • Peer Reviewed
• [Journal Article] The moss Physcomitrella patens as a potential model plant for studying circadian rhythms Bryological Research (2006)
  • Author(s): Setsuyuki Aoki
  • Journal Title: 9(2) Pages: 50-59
  • Peer Reviewed
• [Journal Article] A compact multichannel apparatus for automated real-time monitoring of bioluminescence.
  • Author(s): Kazuhisa Okamoto, Masahiro Ishiura, Tatsuharu Torii, Setsuyuki Aoki
  • Journal Title: Journal of Biochemical and Biophysical Methods (In press)
• [Presentation] ヒメツリガネゴケにおけるCCA1/LHY相同遺伝子PpCCA1aとPpCCA1bの機能解析 (2009)
  • Author(s): 岡田龍、Santosh Satbhai、近藤紗代、青木摂之
  • Organizer: 第50回日本植物生理学会念会
  • Place of Presentation: 名古屋大学共通教育棟、名古屋市
• [Presentation] Characterization of the PRR(Pesudo-Response Regulator)Genes in the Moss Physcomitrella patens (2009)
  • Author(s): Santosh Satbhai, Ryo Okada, Yuki Tezuka, Setsuyuki Aoki
  • Organizer: 第50回日本植物生理学会念会
  • Place of Presentation: 名古屋大学共通教育棟、名古屋市
• [Presentation] Characterization of Homologs of the PRR (Pesudo Response Regulator) Genes in the Moss Physcomitrella patens (2009)
  • Author(s): Santosh B. Satbhai, Ryo Okada, Yuki Tezuka, Tomonori Ito, Setsuyuki Aoki
  • Organizer: Keystone Symposia : Plant Sensing, Response and Adaptation to the Environment
  • Place of Presentation: Big Sky Resort,Big Sky, Montana, USA
• [Presentation] ヒメツリガネゴケにおけるCCAl/LHY相同遺伝子PpCCAlaとPpC CA1bの機能解析 (2009)
  • Author(s): 岡田龍
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学共通教育棟、名古屋市
• [Presentation] Characterization of the PRR (Pesudo-Response Regulator) Genes in the Moss Physcomitrella patens (2009)
  • Author(s): Santosh Satbhai
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学共通教育棟、名古屋市
• [Presentation] Characterization of Homologs of the PRR (Pesudo Response Regulator) Genes in the Moss Physcomitrella patens (2009)
  • Author(s): Santosh Satbhai
  • Organizer: Keystone Symposia : Plant Sensing, Response and Adaptation to the Environment
  • Place of Presentation: Big Sky Resort, Big Sky, Montana, USA
• [Presentation] Functional Analysis of the CCA1/LHY Homolog Genes PpCCA1a and PpCCA1b in the Moss Physcomitrella patens (2008)
  • Author(s): Ryo Okada, Santosh B. Satbhai, Sayo Kondo, Setsuyuki Aoki
  • Organizer: The 8th NIBB-EMBL Joint Meeting-Evolution : Genomes, Cell Types and Shapes
  • Place of Presentation: Okazaki Conference Center, Okazaki, Japan
• [Presentation] Characterization of Homologs of the PRR (Pseudo-Response Regulator) Genes in the Moss Physcomitrella patens (2008)
  • Author(s): Santosh Satbhai, Ryo Okada, Yuki Tezuka, Tomonori Ito, Setsuyuki Aoki
  • Organizer: The 8th NIBB-EMBL Joint Meeting-Evolution: Genomes, Cell Types and Shapes
  • Place of Presentation: Okazaki Conference Center, Okazaki, Japan
• [Presentation] Characterization of the CIRCADIAN CLOCK ASSOCIATED 1/LATE ELONGATED HYPOCOTYL homolog genes PpCCA1a/PpCCA1b and the clock-controlled nuclear-encoded plastid sigma factor gene PpSig5 in the moss Physcomitrella patens (2008)
  • Author(s): Ryo Okada, Kazuhiro Ichikawa, Santosh B. Satbhai, Setsuyuki Aoki
  • Organizer: (poster) IPR Seminar 2008-The Ins and Outs of Chloroplasts
  • Place of Presentation: Icho Kaikan (3F Lecture Hall), Institute for Protein Research, Oasaka University
• [Presentation] ヒメツリガネゴケPhyscomitrella patensにおけるCCA1相同遺伝子の機能解析 (2008)
  • Author(s): 岡田龍、Santosh B. Satbhai、近藤紗代、青木摂之
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋大学IB情報館、名古屋市
• [Presentation] ヒメツリガネゴケのPseudo-Response Regulator(PRR)遺伝子ホモログの同定と解析 (2008)
  • Author(s): Santosh B. Satbhai、岡田龍、手塚裕紀、伊藤智規、青木摂之
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋大学IB情報館、名古屋市
• [Presentation] Characterization of Homologs of the PRR (Pseudo-Response Regulator) Genes in the Moss Physcomitrella patens (2008)
  • Author(s): Santosh Satbhai
  • Organizer: The 8th NIBB-EMBL Joint Meeting-Evolution : Genomes, Cell Types and Shapes
  • Place of Presentation: Okazaki Conference Ce nter, Okazaki, Japan
• [Presentation] Characterization of the CIRCADIAN CLOCK ASSOCIATED l/LATE ELONGATED HYPOCOTYL homolog genes PpCCAla/PpCCAlb and the clock-controlled nuclear-encoded plastid sigma factor gene PpSig5 in the moss Physcomitrella patens (2008)
  • Author(s): Ryo Okada
  • Organizer: IPR Seminar 2008-The Ins and Outs of Chlo roplasts
  • Place of Presentation: Icho Kaikan (3F Lecture Hall), Institute for Protein Research, Osaka University, Suita, Japan
• [Presentation] ヒメツリガネゴケのPseudo-Response Regulator (PRR)遺伝子ホモログの同定と解析 (2008)
  • Author(s): Santosh Satbhai
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋大学IB情報館、名古屋市
• [Presentation] ヒメツリガネゴケ(Physcomitrella patens)におけるCCA1相同遺伝子の解析 (2007)
  • Author(s): 岡田龍、近藤紗代、青木摂之
  • Organizer: 基礎生物学研究所研究会テクニカルワークショップ「コケ植物の実験生物学」
  • Place of Presentation: 岡崎コンファレンスセンター中会議室、岡崎市
• [Presentation] ヒメツリガネゴケの時計関連遺伝子ホモログの系統解析 (2007)
  • Author(s): 青木摂之、岡田龍、Santosh B. Satbhai、手塚裕紀
  • Organizer: 基礎生物学研究所研究会テクニカルワークショップ「コケ植物の実験生物学」
  • Place of Presentation: 岡崎コンファレンスセンター中会議室、岡崎市
• [Presentation] ヒメツリガネゴケの生物時計関連遺伝子群の解析 (2007)
  • Author(s): 青木摂之、岡田龍、手塚裕紀、市川和洋、Santosh B. Satbhai
  • Organizer: 日本遺伝学会第79回大会
  • Place of Presentation: 岡山大学創立50周年記念館(津島キャンパス)、岡山市
• [Presentation] Characterization of circadian clock-related genes in the moss Physcomitrella patens (2007)
  • Author(s): Setsuyuki Aoki, Ryo Okada, Yuki Tezuka, Kazuhiro Ichikawa, Santosh B. Satbhai
  • Organizer: The 2nd World Congress of Chlonobiology
  • Place of Presentation: Keio Plaza Hotel, Tokyo
• [Presentation] ヒメツリガネゴケにおけるCCA1相同遺伝子の解析 (2007)
  • Author(s): 岡田龍、近藤紗代、伊藤智規、青木摂之
  • Organizer: 第47回日本植物生理学会年会
  • Place of Presentation: 愛媛大学、松山
• [Presentation] ヒメツリガネゴケ(Physcomitrella patens)の時計遺伝子群の解析 (2007)
  • Author(s): 青木摂之、岡田龍、近藤紗代、手塚裕紀、伊藤智規
  • Organizer: 第47回日本植物生理学会年会
  • Place of Presentation: 愛媛大学、松山
• [Presentation] Characterization of circadian clock-related genes in the moss Physcomitrella patens (2007)
  • Author(s): Setsuyuki Aoki
  • Organizer: The 2nd World Congress of Chlonobiology
  • Place of Presentation: Keio Plaza Hotel, Tokyo.
• [Presentation] ヒメツリガネゴケにおける時計遺伝子ホモログの解析 (2006)
  • Author(s): 青木摂之、岡田龍、近藤沙代、手塚裕紀、市川和洋、伊藤智規
  • Organizer: 第13回日本時間生物学会学術大会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] ヒメツリガネゴケの概日時計遺伝子ホモログの解析 (2006)
  • Author(s): 青木摂之、岡田龍、近藤紗代、手塚裕紀、市川和洋、伊藤智規
  • Organizer: 日本遺伝学会第78回大会
  • Place of Presentation: つくば国際会議場(エポカルつくば)、つくば
• [Presentation] 生物時計の多様性と生態機能に関する研究のトピックス (2006)
  • Author(s): 青木摂之
  • Organizer: 「概日時計の適応的意義-植物の場合-」シンポジウム
  • Place of Presentation: 琉球大学・熱帯生物圏研究センター・瀬底実験所、沖縄
• [Book] Bacterial Circadian Programs (2009)
  • Author(s): Setsuyuki Aoki
  • Publisher: Springer
• [Book] The Moss Physcomitrella patens (2009)
  • Author(s): Mamoru Sugita
  • Publisher: Blackwell Publishing
• [Book] 光合成研究法 (2009)
  • Author(s): 杉田護
  • Publisher: 北海道大学低温科学研究所
• [Book] In Bacterial Circadian Systems (Chapter 16), Circadian clocks in Synechocystis PCC 6803, Thermosynechococcus elongatus, Trichodesmium spp. and Prochlorococcus spp.(Edited by Jayna Ditty, Shannon Mackey and Carl H. Johnson.) (2008)
  • Author(s): Setsuyuki Aoki and Kiyoshi Onai
  • Publisher: Springer(In press)
• [Book] In the Moss Physcomitrella patens (Chapter 8), Chloroplasts(Edited by Celia Knight, Pierre-Francois Perround and David Cove.) (2008)
  • Author(s): Mamoru Sugita,and Setsuyuki Aoki
  • Publisher: Blackwell Publishing. (In press)
• [Book] リズム生態学 : 体内時計の多様性とその生態機能(清水勇・大石正(編)) (2008)
  • Author(s): 青木摂之
  • Publisher: 東海大学出版会
• [Book] 時間生物学事典(石田直理雄・本間研一(編)) (2008)
  • Author(s): 青木摂之
  • Publisher: 朝倉書店
• [Book] 光合成研究法(伊藤繁ほか(編)) (2008)
  • Author(s): 杉田護、青木摂之
  • Publisher: 北海道大学低温科学研究所(In press)
• [Book] リズム生態学 : 体内時計の多様性とその生態機能 (2008)
  • Author(s): 青木摂之
  • Publisher: 東海大学出版会
• [Book] 時間生物学事典 (2008)
  • Author(s): 青木摂之
  • Publisher: 朝倉書店
• [Book] リズム生態学:体内時計の多様性とその生態機能 (2008)
  • Author(s): 青木摂之(分担執筆)
  • Total Pages: 23
  • Publisher: 東海大学出版会
• [Remarks]
  • URL: http://www.info.human.nagoya-u.ac.jp/~aoki/