Evolutionary analysis on regulatory mechanism of the Dlx3-7 gene cluster regarding evolution of morphological novelty.
| Project/Area Number |
18570007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
SUMIYAMA Kenta National Institute of Genetics, Department of Population Genetics, Assistant professor (00370114)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,910,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Development / Differentiation / Regulation of gene expression / Evolution / Gene / Genome / Transgenic mice / Evo-devo / cis-element |
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| Research Abstract |
The mammalian D1×3 and D1×7 genes are organized as a bigene cluster, and their respective expression patterns are controlled temporally and spatially by cis-elements that have been identified in the intergenic region of the cluster. Previous work showed that one of the conserved elements (137-2) was indeed a visceral arch enhancer that was able to recapitulate the nested expression pattern. We have extended these analyses to include twelve additional mammalian taxa (including a marsupial and a monotreme) in order to study evolution of the non-coding functional elements in mammals. Large-scale sequence comparisons and subsequent conservative motif extraction by the evolutionary rigidity assay (ERA) showed that each cis-element motif exhibited different degrees of evolutionary conservation among mammalian species. 137-1 harbored IL very old conservative motif that was shared by amphioxus distal-less gene. On the other hand, 137-2 exhibited less conservation between monotreme and marsupial/eutherian ancestor despite being highly conserved among marsupial/eutherian species. Since 137-2 has been shown to be important for the craniofacial expression, this intermittent evolution of 137-2 element may possibly be related to craniofacial innovation of mammals.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Possible involvement of SINEs in mammalian-specific brain formation.2008
Author(s)
Takeshi Sasaki, Hidenori Nishihara, Mika Hirakawa, Koji Fjimura, Mikiko Tanaka, Nobuhiro Kokubo, Chiharu Kimura-Yoshida, Isao Matsuo, Kenta Sumiyama, Naruya Saitou, Tomomi Shimogori, Norihiro Okada.
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America Peer reviewed 105
Pages: 4220-1225
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Members of a novel gene family, Gsdm, are expressed exclusively in the epithelium of the skin and gastrointestinal tract in a highly tissue-specific manner.2007
Author(s)
Masaru Tamura, Shigekazu Tanaka, Tomoaki Fujii, Aya Aoki, Hiromitu Komiyama, Kiyoshi Ezawa, Kenta Sumiyama, Tomoko Sagai, Toshihiko Shiroshi.
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Journal Title
Genomics. Peer reviewed(2007) 89(5)
Pages: 618-629
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A series of ENU-induced single base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud.2006
Author(s)
Hiroshi Masuya, Hideki Sezutsu, Yoshiyuki Sakurba, Tomoko Sagai, Masaki Hosoya, Hideki Kaneda, Ikuo Miura, Kimio Kobayashi, Kenta Sumiyama, Aya Shimizu, Junko Nagano, Haruka Yokoyama, Satoko Kaneko, Noriko Sakurai, Yuka Okagaki, Tetsui Noda, Shigeharu Wakana, Yoichi Gondo, Toshihiki Shiroishi.
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Journal Title
Genomics. Peer reviewed(2007) 89(2)
Pages: 207-14
Description
「研究成果報告書概要(欧文)」より
Related Report
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