| Budget Amount *help |
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Research Abstract |
Comparative sequence analyses have identified highly conserved genomic DNA sequences, including noncoding sequences, between humans and other species. By performing whole-genome comparisons of human and mouse, we have identified 611 conserved noncoding sequences longer than 500 by with more than 95% identity between the species. The LCNS were distributed throughout the genome except for the Y chromosome and often occurred in clusters within regions with a low-density of coding genes. Many of the LCNS were also highly conserved in other mammals, chickens, frogs, and fish ; however, we were unable to fmd orthologous sequences in the genomes of invertebrate species. In order to examine whether these conserved sequences are functionally important or merely mutational cold spots, we directly measured the frequencies of ENU-induced germline mutations in the LCNS of the mouse. The mutation frequencies were equivalent to those found in other genomic regions, including coding sequences and introns, suggesting that the LCNS are not mutational cold spots at all. Taken together, these results suggest that mutations occur with equal frequency in LCNS but are eliminated by natural selection during the course of evolution.
We screened ENU-induced mutations in Medaka genomic DNA library by Temperature Gradient Capillary Electrophoresis method. From total 19, 444, 664 by screening, we found 46 mutations in 9 LCNS in Medaka.
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