| Budget Amount *help |
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
The plastid, one of the major organelles in the plant cell, has its own genome. Chloroplast, which is a one form of plastid, capture light energy to conserve free energy in the form of ATP and reduce NADP to NADPH to produce sugars. In this research, we aimed to clarify the mechanisms of plant cell death regulated by plastid. Arabidopsis Cdf1 was originally isolated as a cell death inducer in yeast. In Arabidopsis genome, there are three Cdf-related genes (Cdf1-3). T-DNA inserted plants demonstrated that the heterozygous Cdf1 mutant showed normal vegetative phenotypes under optimal growth conditions, but embryos homozygous for Cdf1 arrested at the globular stage. Such phenotype was complemented by the overexpression of Cdf1. To investigate the gene expression of Cdf1 during embryogenesis, promoter-GUS assay was performed. The 5 kb genomic fragment containing Cdf1 promoter region and coding region was connected with a reporter gene, GUS. Resultant plasmid was introduced into Agrobacterium, and transformed to Arabidopsis. GUS assay demonstrated that Cdf1B expressed during a stage between globular and heart stages. These data indicate that plastid envelope protein Cdf1 is essential for embryogenesis. Furthermore, T-DNA inserted plants in Cdf1-3 demonstrated delayed phenotype in dark-induced leaf senescence. Further biochemical analysis will clarify the molecular function of Cdf genes in plant.
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