Analyses on the novel functions of the COP9 signalosome, a master regulator responding to environm ental stimuli
Project/Area Number |
18570041
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
植物生理・分子
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Research Institution | Kyoto University |
Principal Investigator |
TSUGE Tomohiko Kyoto University, Institute for Chemical Research, Assistant Professor (50291076)
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Project Period (FY) |
2006 – 2007
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Project Status |
Completed (Fiscal Year 2007)
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Budget Amount *help |
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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Keywords | signal transduction / photomorphogenesis / proteolysis / transcriptional regulation / plants / mRNA maturation / protein-protein interaction / environmental stimuli response / タンパク質相互作用 |
Research Abstract |
We have previously reported that COP9 signalosome (CSN) regulates signal transduction by mediating proteolysis in the nuclei. We have also identified novel CSN1 function that does not involve proteolysis in its regulation. Here, we focused on identifying proteins that directly bind to the functional portion of the CSN1 protein, using the Arabidopsis system to investigate the novel regulation of CSN1. The findings are summarized below. 1. The putative CSN1-binding protein, SAP130 (spliceosome associated protein 130) was identified in a systematic pull-down using the functional domain of CSN1 as bait. In Arabidopsis, SAP130 was coded by two closely mapped genes, and its mRNA was detected in all of the tested organs. Plants harboring T-DNA insertions in the promoter or coding region of these genes did not display detectable changes. In attempt to analyze the loss-of-function and gain-of-function of these genes, we constructed transgenic plants that knock-down and over-express the two genes
… More
, by over-expression and by RNAi methods, together with an inducible system. Further analyses are in progress. 2. SAP130 was shown to directly bind CSN1 in both mammalian and plant systems. This binding was mediated through the N-terminal half of CSN1. Plants expressing mutations in this binding-interface have been created to reveal the endogenous nature of this binding. Furthermore we revealed that, SAP130 is homologous to DDB1, directly binds cullin subunit of Cullin-RING ubiquitin E3 ligases, and possess polyubiquitinating activity. 3. Since SAP130 has been identified in multiple complexes such as SF3b, STAGA, and TFTC, we tagged CSN1 and SAP130 with various fluorescence tags to investigate the localization patterns in detail, in combination with external stimuli. The proteins were dominantly detected in the nucleus and further analyses is in progress. These results have been reported through international and domestic conferences and through high-profiled international journal publications. Less
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Report
(3 results)
Research Products
(78 results)
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[Journal Article] SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis.2008
Author(s)
Li L, Qin G, Tsuge T, Hou X, Ding M, Aoyama T, Oka A, Chen Z, Gu H, Zhao Y, Qu LJ.
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Journal Title
The New Phytologist (印刷中)
Description
「研究成果報告書概要(和文)」より
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Peer Reviewed
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[Journal Article] Targeted degradation of ICK4/KRP6 by the RING type E3 ligases RHF1a and RHF2a is essential for mitotic cell cycle progression during Arabidopsis gametogenesis.2008
Author(s)
Liu J, Zhang Y, Qin G, Tsuge T, Sakaguchi N, Luo G, Sun K, Shi D, Aki S, Zheng N, Aoyama T, Oka A, Yang W, Umeda M, Xie Q, Gu H, Qu LJ.
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Description
「研究成果報告書概要(和文)」より
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Peer Reviewed
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[Journal Article] The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis2008
Author(s)
Li, L., Hou, X., Tsuge, T., Ding, M., Aoyama, T., Oka, A., Gu, H., Zhao, Y., Qu, LJ
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Journal Title
Plant Cell Reports 27
Pages: 575-584
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The Arabidopsis phosphatidylinositol phosphate 5-kinase PIP5K3 is a key regulator for root hair tip growth2008
Author(s)
Kusano, H., Testerink, C., Vermeer, JEM., Tsuge, T., Shimada, H., Oka, A., Munnik, T., Aoyama, T
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Journal Title
The Plant Cell 20
Pages: 367-380
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis2008
Author(s)
Li, L., Qin, G., Tsuge, T., Hou, X., Ding, M., Aoyama, T., Oka, A., Chen, Z., Gu, H., Zhao, Y., Qu, LJ
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Journal Title
The New Phytologist (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Targeted degradation of ICK4/KRP6 by the RING type E3 ligases RHF1a and RHF2a is essential for mitotic cell cycle progression during Arabidopsis gametogenesis2008
Author(s)
Liu, J., Zhang, Y., Qin, G., Tsuge, T., Sakaguchi, N., Luo, G., Sun, K., Shi, D., Aki, S., Zheng, N., Aoyama, T., Oka, A., Yang, W., Umeda, M., Xie, Q., Gu, H., Qu, LJ
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Journal Title
The Plant Cell (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis.2008
Author(s)
Li L, Qin G, Tsuge T, Hou X, Ding M, Aoyama T, Oka A, Chen Z, Gu H, Zhao Y, Qu LJ.
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Journal Title
The New Phytologist (in press)
Related Report
Peer Reviewed
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[Journal Article] Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin conjugating enzymes2007
Author(s)
Pick, E., Lau, OS., Tsuge, T., Menon, S., Tong, Y., Dohmae, N., Plafker, S., Deng, XW., Wei, N
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Journal Title
Molecular and Cellular Biology 27
Pages: 4708-4719
Description
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[Journal Article] A calmodulin-binding mitogen-activated protein kinase phosphatase is induced by wounding and regulates the activities of stress-related mitogen-activated protein kinases in rice2007
Author(s)
Katou, S., Kuroda, K., Seo, S., Yanagawa, Y., Tsuge, T., Yamazaki, M., Miyao, A., Hirochika, H., Ohashi, Y
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Journal Title
Plant & Cell Physiology 48
Pages: 332-344
NAID
Description
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[Presentation] Mammalian DET1 regulates CUL4A activity and forms stable complexes with ubiquitin E2 enzymes2007
Author(s)
Pick, E., Lau, OS., Tsuge, T., Deng, XW., Wei, N
Organizer
The Annual Meeting of the Israel Society for Biochemistry & Molecular Biology
Place of Presentation
The Technion, Haifa, ISRAEL
Year and Date
2007-08-10
Description
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[Presentation] ARR1 directly activates cytokinin response genes that encode proteins with diverse regulatory functions2007
Author(s)
Taniguchi, M., Tsuge, T., Aoyama, T., Oka, A
Organizer
Trends in Plant Hormones, RIKEN Plant Science Center International Symposium
Place of Presentation
RIKEN Yokohama Institute, Yokohama, JAPAN
Description
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[Presentation] Identifying novel regulation beyond proteolysis of the COP9 signalosome2007
Author(s)
Tsuge, T., Aki, S., Taniguchi, M., Dohmae, N., Menon, S., Pick, E., Wei, N., Oka, A
Organizer
The 48th Annual meeting of The Japanese Society of Plant Physiologists
Place of Presentation
Ehime University, Matsuyama, JAPAN
Description
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[Presentation] The DDB1-like protein SAP130/Sf3b-3 is a cullin binding protein, and is regulated by the neddylation pathway2007
Author(s)
Menon, S., Tsuge, T., Dohmae, N., Takio, K., Wei, N
Organizer
The Ubiquitin family-Cold Spring Harbor Laboratory Meeting
Place of Presentation
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY USA
Description
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[Presentation] Identifying novel regulation through the association of mRNA processing factors and COP9 signalosome2007
Author(s)
Aki, S., Kataoka, M., Taniguchi, M., Dohmae, N., Menon, S., Wei, N, Oka, A., Tsuge, T
Organizer
The 49th Annual meeting of The Japanese Society of Plant Physiologists
Place of Presentation
Sapporo Convention Center, Sapporo, JAPAN
Description
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[Presentation] COP9シグナロソームの新規制御機構2006
Author(s)
安喜史織、岡穆宏、柘植知彦
Organizer
第106回化学研究所研究発表会
Place of Presentation
京都大学化学研究所、京都府宇治市
Year and Date
2006-12-15
Description
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[Presentation] COP9 Signalosome Subunit1 interacts with components of the transcription/RNA processing complexes.2006
Author(s)
Tsuge T, Aki S, Taniguchi M, Dohmae N, Menon S, Pick E, Wei N, Oka A.
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University Campus, New Haven, CT USA
Description
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[Presentation] Regulation by the COP9 Signalosome of AtPIC2, a novel F-box protein from Arabidopsis thaliana.2006
Author(s)
Iafrate S, Moubayidin L, Heyl A, Tsuge T, Deng XW, Costantino P, Serino G.
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University Campus, New Haven, CT USA
Description
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[Presentation] Human DET1 complexes contain canonical ubiquitin E2 enzymes and regulate CUL4A E3 activity.2006
Author(s)
Pick E, Tsuge T, Tong Y, Menon S, Dohmae N, Deng XW, Wei N.
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University Campus, New Haven, CT USA
Description
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[Presentation] Regulation by the COP9 Signalosome of PIC2, a novel F-box protein from Arabidopsis thaliana2006
Author(s)
Moubayidin, L., Iafrate, S., Tsuge, T., Heyl, A., Deng, XW., Costantino, P., Serino, G
Organizer
3rd EPSO Conference '06 "Plant Dynamics : from Molecules to Ecosystems"
Place of Presentation
Thermal Hotel, Visegrad, HUNGARY
Description
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[Presentation] COP9 Signalosome Subunitl interacts with components of the transcription/RNA processing complexes.2006
Author(s)
Tsuge, T., Aki, S., Taniguchi, M., Dohmae, N., Menon, S., Pick, E., Wei, N., Oka, A
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University, New Haven, CT USA
Description
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[Presentation] Regulation by the COP9 Signalosome of AtPIC2, a novel F-box protein from Arabidopsis thaliana2006
Author(s)
Iafrate, S., Moubayidin, L., Heyl, A., Tsuge, T., Deng, XW., Costantino, P., Serino, G
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University, New Haven, CT USA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cullin Neddylation and functions of CSN1 and CSN82006
Author(s)
Menon, S., Pick, E., Tsuge, T., Dohmae, N., Flavell, R., Wei, N
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University, New Haven, CT USA
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Human DET1 complexes contain canonical ubiquitin E2 enzymes and regulate CUL4AE3 activity2006
Author(s)
Pick, E., Tsuge, T., Tong, Y., Menon, S., Dohmae, N., Deng XW., Wei, N
Organizer
ZOMES-IV The Fourth International Symposium on COP9 Signalosome, Proteasome, and eIF3
Place of Presentation
Yale University, New Haven, CT USA
Description
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[Presentation] Novel regulation of COP9 Signalosome : the master regulator of signal transduction2006
Author(s)
Tsuge, T., Ala, S., Taniguchi, M., Dohmae, N., Menon, S., Pick, E., Wei, N., Oka, A
Organizer
Molecular Biology Society of Japan 2006 Forum : Molecular Biology-the Next Decade
Place of Presentation
Nagoya Congress Center, Nagoya, JAPAN
Description
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[Presentation] Interaction studies of CSN1 and SAP1302006
Author(s)
Aki, S., Oka, A., Tsuge, T
Organizer
Molecular Biology Society of Japan 2006 Forum : Molecular Biology-the Next Decade.
Place of Presentation
Nagoya Congress Center, Nagoya, JAPAN
Description
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