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Purification of oxygen-evolving photosystem II complex from a diatom and the binding properties of the extrinsic proteins

Research Project

Project/Area Number 18570049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionTokyo University of Science

Principal Investigator

ENAMI Isao  Tokyo University of Science, Faculty of Science, Professor (40084305)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsPhotosynthesis / Diatom / Photosystem II complex / Oxygen evolution / Extrinsic proteins / Freeze-thawing / Thylakoid membrane / Photosystem II core complex / 光化学系2複合体 / 酸素発生系 / PS2 core / 紅藻
Research Abstract

Diatoms are unicellular photosynthetic algae found throughout the world's oceans and freshwater, and constitute one of the most important producers of the phytoplankton communities in aquatic ecosystems and the global carbon cycle. In spite of their significance, little is known about Photosystem II (PSII) in diatoms. In this study, we succeeded for the first time in the preparation of thylakoid membranes and PSII particles retaining a high oxygen-evolving activity and all of the extrinsic proteins from a diatom, Chaetoceros gracilis. The diatom thylakoid membranes and PSII particles showed an oxygen - evolving activity of about 250 and 850 μmol 02/mg Chl/h in the absence of CaC12, respectively. The PSII particles contained fucoxanthin chlotophyll a/c-binding proteins in addition to main intrinsic proteins of CP47, CP43, 02, D1, cytochrome b559, and the anntena size was estimated to be 229 Chl a per 2 molecules of pheophytin. Five extrinsic proteins were 〓oichimoterically released from the diatom PSII particles by alkaline Tris-treatment.
Among these five extrinsic proteins, four proteins were red algal-type extrinsic proteins, namely, PsbO, PsbQ', PsbV and PsbU, whereas the other one was a novel, hypothetical protein. The gene encoding the novel extrinsic protein was cloned and sequenced. The deduced proteins contained three characteristic leader sequences targeted for chloroplast endoplasmic reticulum, chloroplast envelope and thylakoid membrane, respectively, indicating that the novel protein is encoded in the nuclear genome and constitutes one of the extrinsic proteins located in the lumenal side. The binding properrties and functions of the five extrinsic proteins will be examined by release-reconstitution experiments of their extrinsic proteins with PSII.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (40 results)

All 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 7 results) Presentation (28 results)

  • [Journal Article] Aromatic structure of Tyrosine-92 in the extrinsic PsbI protein of red algal photosystem II is important for its functioning2007

    • Author(s)
      Okumura A., Enami I., et. al.
    • Journal Title

      FEBS Lett. 581

      Pages: 5255-5258

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bio-photosensor:Cyanobacterial photosystem I coupled with transistor viamolecular wire2007

    • Author(s)
      Terasaki N., Enami I., et. al.
    • Journal Title

      Biochim.Biophys.Acta Bioenergetic 17 67

      Pages: 653-659

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of oxygen-evolving thylakoid membranes and photosystemII particles from a marine diatom Chaetoceros gracilis2007

    • Author(s)
      Nagao R., Enami I., et. al.
    • Journal Title

      Biochim.Biophys.Acta Bioenergetic 17 67

      Pages: 1353-1362

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Quality control of photosystem II:cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids2007

    • Author(s)
      Komayama K., Enami I., et. al.
    • Journal Title

      Biochim.Biophys.Acta Bioenergetic 17 67

      Pages: 838-846

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Aromatic structure of Tyrosine-92 in the extrinisc PsbI protein of red algal photosystem II is important for its functioning2007

    • Author(s)
      Okumura, A., Enami, I., et. al.
    • Journal Title

      FEBS Lett 581

      Pages: 5255-5258

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bio-photosensor : Cyanobacterial photosystem I coupled with transistor via molecular wire2007

    • Author(s)
      Terasaki, N., Enami, I., et. al.
    • Journal Title

      Biochim. Biophys. Acta 1767

      Pages: 653-659

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a marine diatom Chaetoseros gracilis2007

    • Author(s)
      Nago, R., Enami, I., et. al.
    • Journal Title

      Biochim. Biophys. Acta 1767

      Pages: 1353-1362

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Quality control of photosystem I : cleavage and aggregation of heat-damaged DI protein in spinach thylakoids2007

    • Author(s)
      Komayama, K., Enami, I., et. al.
    • Journal Title

      Biochim. Biophys. Acta 1767

      Pages: 838-846

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of redalgal photosystem II is important for its functioning2007

    • Author(s)
      Okumura A., Enami I., et. al.
    • Journal Title

      FEBS Lett. 581

      Pages: 5255-5258

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bio・photosensor: Cyanobacterial photosystem I coupled with transistor via molecular wire2007

    • Author(s)
      Terasaki N., Enami I., et. al.
    • Journal Title

      Biochim. Biophys. Acta Bioenergetic 1767

      Pages: 653-659

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a marine diatom Chaetoceros gracilis2007

    • Author(s)
      Nagao R., Enami I., et. al.
    • Journal Title

      Biochim. Biophys. Acta Bioenergetic 1767

      Pages: 1353-1362

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bio-photosensor : Cyanobacterial photosystem I coupled with transistor via molecular wire2007

    • Author(s)
      Terasaki N, Enami I et al.
    • Journal Title

      Biochim. Biophys. Acta Bioenergetic (In press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] ラン色細菌Synechocystis sp PCC6803における酸性ストレス応答遺伝子slr0967欠損株における発現調節2008

    • Author(s)
      太田 尚孝、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-03-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 紅藻Cyanidium caldarium由来光化学系II複合体結晶のX線回析分解能の改善2008

    • Author(s)
      足立 秀行、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-03-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ホウレンソウPsbPがPSII複合体へ結合するために必要なリジン残基の同定2008

    • Author(s)
      奥村 彰規、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-03-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 珪藻PSII core複合体を構成するCP47,CP43,D1/D2 coreの分離とそれらに結合した色素組成2008

    • Author(s)
      長尾 遼、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-03-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 珪藻PSII core複合体を構成するCP47, P43, D1/D2 coreの分離とそれらに結合した色素組成の分析2008

    • Author(s)
      長尾遼、榎並勲, 他
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-03-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] ホウレンソウPsbPがPSII複合体へ結合するために必要なリジン残基の同定2008

    • Author(s)
      奥村彰規、榎並勲, 他
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-03-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] 紅藻Cyanidium caldarium由来光化学系II複合体結晶のX線回折分解能の改善2008

    • Author(s)
      足立秀行、榎並勲, 他
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン色細菌Synechocystis sp PCC6803における酸性ストレス応答遺伝子slr0967欠損株による発現調節2008

    • Author(s)
      太田尚孝、榎並勲, 他
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン色細菌Synechocystis sp PCC6803における酸性ストレスに特異的に応答する遺伝子(slr0967とs110939)の解析2007

    • Author(s)
      森山 淳、榎並 勲, 他
    • Organizer
      日本植物学会
    • Place of Presentation
      東京理科大学野田校舎
    • Year and Date
      2007-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 紅藻PSIIに結合した表在性PsbUの部位特異的変異による機能ドメインの同定2007

    • Author(s)
      佐野 正典、榎並 勲, 他
    • Organizer
      日本植物学会
    • Place of Presentation
      東京理科大学野田校舎
    • Year and Date
      2007-09-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 珪藻の酸素発生光化学系2標品の単離とその表在性蛋白2007

    • Author(s)
      長尾 遼、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 紅藻Cyanidium caldarium由来光化学系II複合体の精製・結晶化2007

    • Author(s)
      足立 秀行、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 珪藻PSII標品に存在する5種の表在性蛋白のクローニングと解析2007

    • Author(s)
      奥村 彰規、榎並 勲, 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Quality control of Photosystem II: the fate of heat-damaged D1 protein in spinach thylakoids depends on the distribution and action of FtsH2007

    • Author(s)
      K. Kanayama, I. Enami, et. al.
    • Organizer
      日本植物生理学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation of the diatom PSII retaining high oxygen-evolving activity from a marine diatom,Chaetoceros gracilis2007

    • Author(s)
      Ryo Nagao, Isao Enami, et. al.
    • Organizer
      14th International congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of functional domain of PsbU in red algai PSII by site-directed mutagenesis2007

    • Author(s)
      Masanori Sano, Isao Enami, et. al.
    • Organizer
      14th International congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Evaluation of acid stress tolerance of mutants of Synechocystis sp PCC6803 lacking signal transduction related gene,sig B.2007

    • Author(s)
      Hisataka Ohta, Isao Enami, et. al.
    • Organizer
      14th International congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and crystallization of photosystem II complex from a red alga Cyanidium caldarium2007

    • Author(s)
      Hideyuki Adachi, Isao Enami, et. al.
    • Organizer
      14th International congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation of the diatom PSII retaining high oxygen-evolving activity from a marine diatom, Chaetoceros gracilis2007

    • Author(s)
      Ryo, Nagao, Isao, Enami, et. al.
    • Organizer
      14th international congress or Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of functional domaine of PsbU in red algal PSII by site-directed mutagenesis2007

    • Author(s)
      Masanori, Sano, Isao, Enami, et. al.
    • Organizer
      14th International congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Evaluation of acid stress torerance of mutants of Synechosystis sp PSS6803 lacking signal transduction related gene, sig B2007

    • Author(s)
      Hisataka, Ohta, Isao, Enami, et. al.
    • Organizer
      14th International congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Purification and crystallization of photosystem II complex from a red alga Cyanidium cladarium2007

    • Author(s)
      Adati H., Enami I., et. al.
    • Organizer
      14th International congress of photosynthesis
    • Place of Presentation
      Glasgow
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of functional domain of PsbU in red algal PSII by site-directed mutagenesis2007

    • Author(s)
      Sano M., Enami I., et. al.
    • Organizer
      14th International congress of photosynthesis
    • Place of Presentation
      Glasgow
    • Related Report
      2007 Annual Research Report
  • [Presentation] Evaluation of acid stress tolerance of mutants of Synechocystis sp. PCC6803 lacking signal transduction related gene, sigB2007

    • Author(s)
      Ohta H., Enami I., et. al.
    • Organizer
      14th International congress of photosynthesis
    • Place of Presentation
      Glasgow
    • Related Report
      2007 Annual Research Report
  • [Presentation] Isolation of the diatom PSII retaining high oxygen-evolving activity from a marine diatom, Chaetoceros gracilis2007

    • Author(s)
      Nagao R., Enami I., et. al.
    • Organizer
      14th International congress of photosynthesis
    • Place of Presentation
      Glasgow
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン色細菌Synechocystis sp. PCC6803における酸性ストレスに特異的に応答する遺伝子(slr0967とsll0939)の解析2007

    • Author(s)
      森山淳、榎並勲, 他
    • Organizer
      日本植物学会第71回大会
    • Place of Presentation
      東京理科大学野田校舎
    • Related Report
      2007 Annual Research Report
  • [Presentation] 紅藻PS2に結合した表在性PsbUの部位特異的変異による機能ドメインの同定2007

    • Author(s)
      佐野正典、榎並勲, 他
    • Organizer
      日本植物学会第71回大会
    • Place of Presentation
      東京理科大学野田校舎
    • Related Report
      2007 Annual Research Report
  • [Presentation] BN-PAGEによる珪藻PS2 coreとFCPの分離とそれらの特性2007

    • Author(s)
      長尾遼、榎並勲, 他
    • Organizer
      日本植物学会第71回大会
    • Place of Presentation
      東京理科大学野田校舎
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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