Molecular structure of sperm flagella in mammal
| Project/Area Number |
18570059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Kyushu University |
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Principal Investigator |
IIDA Hiroshi Kyushu University, Faculty of Agriculture, Associate Professor (70150399)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | spermatozoa / development and differentiation / molecular recognition / genes / 発生・文化 / 鞭毛 / 軸糸 / Tektin / Spetex |
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| Research Abstract |
This study is aimed to elucidate the molecular architecture of mammalian sperm flagella with special reference to Tektin4 and Spetx-1 that have been cloned by differential display and yeast two-hybrid technique. We have disclosed by immnunoelectron microscopy that Tektin4 is present at the surface of outer dense fiber (ODF) and Spetex-1 is a component of satellite fibrils. In addition, we showed that Tektin2 and Tektin5 were localized around ODF and the inner surface of mitochondrial sheath, respectively. We also found that Gcl-2 is associated with the cortex of ODF, like Tektin4. We biochemically studied the localization of these molecules by immunoblot analyses using fractionated samples prepared from purified rat spermatozoa, i.e., plasma membrane, axoneme, ODF, and fibrous sheath. The outcome revealed that all of these molecules were found to be detected in 6M urea-extracted fraction (ODF and ODF-related fractions). When Tektin4 and Spetex-1 were co-transfected into COS-7 cells, the two molecules were found to be co-localized in patch-like structures around nuclei of the cells, the observation suggesting that the two molecules could interact each other in the cells. Finally, we performed yeast two-hybrid screening to isolate molecules that interact with Tektin2 and Tektin5, which resulted in isolation of several novel molecules. Through study of these molecules, we will make a progress for understanding the molecular architecture of mammalian sperm flagella.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Tektin5, a new TEKTIN family member, is a component of the middle piece of flagella in rat spermatozoa2008
Author(s)
Murayama, E., Yamamoto, E., Kaneko, T., Shibata, Y., Inai, T., Iida, H
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Journal Title
Mol Reprod Dev 75
Pages: 650-658
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Factors maintaining normal sperm tail structure during epididymal maturation studied in Gopc-/- mice.2007
Author(s)
Suzuki-Toyota, F., Ito, C., Toyama, Y., Maekawa, M., Yao, R., Noda, T., Iida, H., Toshimori, K
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Journal Title
Biol Reprod 77
Pages: 71-82
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Germ cell-less like 2 protein is a new component of outer dense fibers in rat sperm flagella2007
Author(s)
Murayama, E., Katoh, M., Kanebayashi, A., Kaneko, T., Shibata, Y., Inai, T., Iida, H
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Journal Title
Reproduction 134
Pages: 749-756
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tektin 4 is located on outer dense fibers, not associated with axonemal tubulins of flagella in rodent spermatozoa2006
Author(s)
Iida, H., Honnda, Y., Matsuyama, T., Shibata, Y., Inai, T
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Journal Title
Mol. Reprod.Dev 73
Pages: 929-936
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Gc1-2, a molecule localized at the cortex of outer dense fibers in rat sperm flagella2008
Author(s)
Iida, H., Murayama, E., Kaneko, T., Kawarada, K., Kurio, H., Sata, J., Yano, R
Organizer
The 78th Zoological congress
Place of Presentation
Hirosaki
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Strucutural proteins in sperm flagella, Spetex-1 and Tektin42007
Author(s)
Kaneko, T., Kawarada, K., Kurio, H., Sata, J., Iida, H
Organizer
Kyushu Zoological Congress localization
Place of Presentation
Fukuoka
Year and Date
2007-05-19
Description
「研究成果報告書概要(欧文)」より
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