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Broader understanding of the transduction mechanisms in insect sugar receptors : Asignal transduction pathway involving nitric oxide

Research Project

Project/Area Number 18570068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionThe University of Electro-Communications

Principal Investigator

NAKAMURA Tadashi  The University of Electro-Communications, 電気通信学部, 教授 (50217858)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Atsushi  電気通信大学, 電気通信学部, 助教 (50361829)
Co-Investigator(Renkei-kenkyūsha) OZAKI Mamiko  神戸大学, 大学院・理学研究科, 教授 (00314302)
MURATA Yoshihiro  高知医科大学, 医学部, 助教 (40377031)
Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,220,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords昆虫 / 糖受容細胞 / 苦味物質受容細胞 / 情報変換機構 / 一酸化窒素 / 環状ヌクレオチド / 食欲調節機構 / 吻伸展反射 / ショウジョウバエ / アポトーシス遺伝子 / クロキンバエ / 苦味受容細胞 / GAL4 / UASシステム / 動物生理 / 化学 / セカンドメッセンジャー / 化学感覚
Research Abstract

Relationships between NO-production and the excitation mechanisms in the taste cells and between the taste sensation and appetite regulation were investigated. NO-sensitive guanylyl cyclase (sGC) was detected and suggested to participate in the signal transduction pathways in the sugar cells, while other pathway was detected in the bitterness cells. Meanwhile it was demonstrated that the fly's aversive behavior to sucrose after their feeding sucrose in the limonene fume was due to the formation of long-term memory.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (40 results)

All 2010 2009 2008 2007 2006 Other

All Journal Article (15 results) (of which Peer Reviewed: 10 results) Presentation (18 results) Book (6 results) Remarks (1 results)

  • [Journal Article] Contribution of the inositol 1,4,5-trisphosphate transduction cascade to the detection of "bitter" compound in blowflies2009

    • Author(s)
      Qin Ouyang, Hiroyasu Sato, Yoshihiro Murata, Atsushi Nakamura, Mamiko Ozaki, Tadashi Nakamura
    • Journal Title

      Comparative Biochemistry and Physiology, Part A 153

      Pages: 309-316

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of the inositol 1, 4, 5-trisphosphate transduction cascade to the detection of "bitter" compounds in blowflies2009

    • Author(s)
      Qin Ouyang
    • Journal Title

      Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology 153

      Pages: 309-316

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アカハライモリのリポカリンによる嗅電図の修飾2009

    • Author(s)
      野海芳博
    • Journal Title

      日本味と匂学会誌 16

      Pages: 545-548

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of inositol 1, 4, 5-trisphosphate transduction cascade to the detection of "bitter" compound in blowflys2009

    • Author(s)
      Ouyang Q, Sato H, Murata Y, Nakamura A, Ozaki M, Nakamura T
    • Journal Title

      Comparative Biochemistry and Physiology Part A,(電子版) (doi : 10.1016/j.cbpa.2009.03.004)

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Contribution of the inositol 1, 4, 5-trisphosphate transduction cascade to the detection of “bitter" compounds in blowflies2009

    • Author(s)
      Ouyang, Qin
    • Journal Title

      Comparative Biochemistry and Physiology -Part a : Molecular & Integrative Physiology(電子版) doi : 10. 1016/j. cbpa. 2009. 03. 004

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Odour of limonene affects feeding behaviour in the blowfly, Phormia regina2008

    • Author(s)
      Atsushi Nakamura, Tomoyuki Suzuki, Daiki Taniguchi, Atsushi Matsuda, Manabu Tobeta, Tadashi Nakamura
    • Journal Title

      Neuroscience Letters 446

      Pages: 36-39

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Odour of limonene affects feeding behavior in the blowfly, Phormiaregina.2008

    • Author(s)
      Nakamura, Atsushi
    • Journal Title

      Neuroscience Letters 446

      Pages: 36-39

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クロキンバエ第5味細胞の情報変換機能の解析2007

    • Author(s)
      佐藤博康
    • Journal Title

      日本味と匂学会誌 14

      Pages: 335-338

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ショウジョウバエの味覚受容メカニズム2007

    • Author(s)
      仲村厚志
    • Journal Title

      バイオメカニズム学会誌 31

      Pages: 138-142

    • NAID

      110006838875

    • Related Report
      2007 Annual Research Report
  • [Journal Article] NO-cGMP signaling pathway2007

    • Author(s)
      Tadashi Nakamura
    • Journal Title

      sugar receptor neurons in the blowfly in "Frontiers in Molecular Endocrinology of Invertebrates and Vertebrates, Vol. 2", Department of Animal Sciences, School of Life Scie

      Pages: 1-5

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Primary Culture of Gustatory Receptor Neurons from the Blowfly2006

    • Author(s)
      Yoshihiro Murata, Mamiko Ozaki, Tadashi Nakamura
    • Journal Title

      Phormia regina, Chemical Senses 31

      Pages: 497-504

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Primary culture of gustatory receptor neurons from the blowfly, Phormia regina2006

    • Author(s)
      Yoshihiro Murata, Mamiko Ozaki, Tadashi Nakamura
    • Journal Title

      Chemical Senses 31

      Pages: 497-504

    • Related Report
      2006 Annual Research Report
  • [Journal Article] cDNA cloning and expression analysis of signaling proteins in olfactory sensory system of Japanese common newt, Cynopus pyrhogaster2006

    • Author(s)
      Masataka Takashima, Keiko Okano, Ken Sawada, Tadashi Nakamura, Tatsuo Iwasa
    • Journal Title

      生物物理 46

    • Related Report
      2006 Annual Research Report
  • [Journal Article] クロキンバエ培養味細胞における蔗糖刺激に誘発されるNO産生2006

    • Author(s)
      後藤信太郎, 村田芳博, 尾崎まみこ, 中村整
    • Journal Title

      日本味と匂学会誌 13

      Pages: 287-288

    • NAID

      110006286337

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Odour of limonene affects feeding behavior in the blowfly

    • Author(s)
      Nakamura A, Suzuki T, Taniguchi D, Matsuda A, Tobeta M, Nakamura T
    • Journal Title

      Phormia regina. Neuroscience Letters 446(2008)

      Pages: 36-39

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] ケージド化合物を用いたクロキンバエ味細胞情報変換の研究2010

    • Author(s)
      米本聖夜子
    • Organizer
      日本動物学関東支部第62回大会
    • Place of Presentation
      筑波
    • Year and Date
      2010-03-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] クロキンバエ味細胞における苦味物質の糖応答抑制機構2010

    • Author(s)
      瀬戸美星
    • Organizer
      日本動物学会関東支部第62回大会
    • Place of Presentation
      筑波
    • Year and Date
      2010-03-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] クロキンバエ中枢神経系における摂食調節機能へのAMPKの関与2009

    • Author(s)
      中村整
    • Organizer
      第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会
    • Place of Presentation
      豊中
    • Year and Date
      2009-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] クロキンバエ中枢神経系におけるAMPKβの解析2009

    • Author(s)
      仲村厚志
    • Organizer
      第31回日本比較生理生化学会・第34回日本比較内分泌学会合同大会
    • Place of Presentation
      豊中
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] クロキンバエ中枢神経系における摂食調節機能へのAMPKの関与2009

    • Author(s)
      鈴木智之
    • Organizer
      第31回日本比較生理生化学会・第34回日本比較内分泌学会合同大会
    • Place of Presentation
      豊中
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] アカハライモリのリポカリンによる嗅電図の修飾2009

    • Author(s)
      野海芳博
    • Organizer
      日本味と匂学会
    • Place of Presentation
      旭川
    • Year and Date
      2009-09-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Odour of limonene affects feeding behavior in the blowfly, Phormia regina2008

    • Author(s)
      Nakamura T
    • Organizer
      The 6^<th> international symposium on molecular and neural mechanisms of taste and olfactory perception
    • Place of Presentation
      福岡
    • Year and Date
      2008-12-07
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Odour of limonene affects feeding behavior in the blowfly, Phormia regma2008

    • Author(s)
      Nakamura, Tadashi
    • Organizer
      The 6th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      博多
    • Year and Date
      2008-12-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] クロキンバエ中枢神経系における摂食調節機能へのAMPキナーゼの関与2008

    • Author(s)
      中村, 整
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      博多
    • Year and Date
      2008-09-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Bitter compound detection in the blowfly taste receptor cell : evidence for the inositol 1, 4, 5-trisphosphate-dependent transduction2008

    • Author(s)
      Nakamura, Tadashi
    • Organizer
      International Seminar : Evolutionary Studies in Behavioral Neuroscience
    • Place of Presentation
      葉山
    • Year and Date
      2008-06-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] クロキンバエ唇弁における糖応答の苦味物質による抑制2008

    • Author(s)
      中村整
    • Organizer
      日本動物学会第60回関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] クロキンバエにおけるリモネンガスとショ糖溶液を用いた条件付け方法の確立2008

    • Author(s)
      中村整
    • Organizer
      社団法人日本動物学会第60回関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロキンバエふ節感覚毛における苦味受容機構2008

    • Author(s)
      中村整
    • Organizer
      社団法人日本動物学会第60回関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロキンバエ唇弁における糖応答の苦味物質による抑制2008

    • Author(s)
      中村整
    • Organizer
      社団法人日本動物学会第60回関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Cp-PLBP: A possible ligand-binding protein found in the olfactory epithelium of Cynops pyrrhogaster2007

    • Author(s)
      Tatsuo Iwasa
    • Organizer
      日本生物物理学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロキンバエPhormla reglnaの苦味受容細胞における情報変換機構の研究2007

    • Author(s)
      中村整
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Year and Date
      2007-09-20
    • Related Report
      2009 Final Research Report
  • [Presentation] クロキンバエPhormia reginaの苦味受容細胞における情報変換機構の研究2007

    • Author(s)
      中村整
    • Organizer
      社団法人日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Year and Date
      2007-09-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロキンバエ第5味細胞の情報変換機構の解析2007

    • Author(s)
      中村整
    • Organizer
      日本味と匂学会第41回大会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-27
    • Related Report
      2007 Annual Research Report
  • [Book] SEB Experimental Biology Series Vol 63, Insect Taste, Chapter 4(Chemosensory regulation of feeding in the blowfly : several studies after 'The Hungry fly'2009

    • Author(s)
      Tadashi Nakamura, Mamiko Ozaki
    • Publisher
      Taylor & Francis Group
    • Related Report
      2009 Final Research Report
  • [Book] シリーズ21世紀の動物科学、9巻、動物の感覚とリズム、第4章(化学感覚)2009

    • Author(s)
      中村整、屋崎まみこ
    • Publisher
      倍風館
    • Related Report
      2009 Final Research Report
  • [Book] Chemosensory regulation of feeding in the blowfly : several studies after' The Hungly Fly'(SEB Experimental Biology Series Vol.63. Insect Taste, Chapter 4)2009

    • Author(s)
      Ozaki M, Nakamura T
    • Publisher
      Taylor &amp; Francis Group
    • Related Report
      2008 Self-evaluation Report
  • [Book] Chemosensory regulation of feeding in the blowfly : several studies after ‘The Hungly Fly'(SEB Experimental Biology Series Vol 63. Insect Taste, Chapter 4)2009

    • Author(s)
      Ozaki, Mamiko
    • Publisher
      Taylor & Francis Group
    • Related Report
      2008 Annual Research Report
  • [Book] 化学感覚(シリーズ21世紀の動物科学、9巻、動物の感覚とリズム、第4章)2007

    • Author(s)
      中村整
    • Publisher
      培風館
    • Related Report
      2007 Annual Research Report
  • [Book] NO-cGMP signaling pathway in sugar receptor neurons in the blowfly in "Frontiers in Molecular Endocrinology of Invertebrates and Vertebrates, Vol.2"2007

    • Author(s)
      Tadashi Nakamura
    • Publisher
      Department of Animal Sciences, School of Life Sciences, University of Hyderabad
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://kaeru.pc.uec.ac.jp/

    • Related Report
      2009 Final Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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