Studies on Diversity and Universality in Sperm Activation and Chemotaxis at Fertilization

• Project/Area Number: 18570080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Yamagata University
• Principal Investigator: MORISAWA Masaaki Yamagata University, Faculty of Science, Professor (40013594)
• Co-Investigator(Kenkyū-buntansha): WATANABE Akihiko Yamagata University, Faculty of Science, Associate Professor (30250913) ; HISINUMA Tasuku Yamagata University, Faculty of Science, Lecturer (70181097) ; YOSHIDA Manabu The University of Tokyo, Graduate School of Science, Lecturer (60301785) ; MOTOMURA Taizou Hokkaido University, Graduate School of Science, Professor (30183974) ; IWAMOTO Teruaki International University of Health and Welfare, 国際医療福祉大学, Professor (60046117)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,080,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: sperm activation / sperm chemotaxis / human / amphibians / sea cucumber / starfish / ascidians / brown algae / 精子運動活性化・走化性 / ヒト卵胞液 / ホヤ類種特異性 / 高速液体クロマトグラフィー(HPLC) / 質量分析 / 境界動物 / ナメクジウオ

Research Abstract

Sperm activating substance derived from human follicular fluid was partially purified, but chemical structure of it is still obscure. The role of semenogelin and zinc ions for the initiation of human sperm motility was investigated and the obtained results were publishing in the international scientific journal "Human Reproduction". Purification and identification of molecular structure of sperm activating peptides were tried in newt. Furthermore, the interesting results that newt derived sperm activating peptide also activates sperm of salamander and primitive toad were obtained by collaboration work with Italian scientists. Immuno-histochemical analysis showed that the sperm activating peptides from newt, salamander and toad are similar immunological properties. Collaboration works with American scientists showed that calucium ions has an important role in the cell signaling mechanism for sperm activation in newt. In regards with species specificity in sperm activation and chemotaxis in ascidians, there was clear species specificity between genus. Furthermore, Purification processes of sperm activating and attracting factors were successfully proceeded in the two ascidian species, Phallusia mamillata and Ascidia sydneiensis. Sperm activating substance from jelly layer of sea cucumber eggs was partially purified, and it was identified that the substance will be small peptide. Physiological analyses on the-mechanism of the initiation of sperm motility in starfish have clearly shown that a zinc binding enzyme, carbonic anhydrase regulates intracellular pH by controlling C0_2/HCO_3 for triggering the phenomenon. We finally tried to investigate the outline of cell signaling for sperm activation and chemotaxis in brown algae using software for sperm motility analysis, Boh Boh.

Research Products

• [Journal Article] Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans (2008)
  • Author(s): Yoshida, K, ・Kawano, N, ・Yoshiike, M, ・Yoshida, M, ・Iwamoto, T, ・Morisawa, M
  • Journal Title: Mol. Human Reprod. 14 Pages: 151-156
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Adaptation and stategy for fertilization in the sperm of teleost fishes. (2008)
  • Author(s): Morisawa, M.
  • Journal Title: Jounal Applied Ichthyology 24 Pages: 362-370
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of two egg-dericed molecules in motility initiation and fertilization in the Pacific herring(Clupea pallasi). (2008)
  • Author(s): Cherr, GN., ・Morisawa, M., ・Vines, CA., ・Yoshida, K., ・Smith, EH, ・Matsubara, T, ・Pillai, MC., ・Griffin, FJ.・Yanagimachi, R.
  • Journal Title: Intern. J. Develop. Biol, (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Motility activation and chemotaxis in ascidian sperm. (2008)
  • Author(s): Morisawa, M., ・Yoshida, M., ・lnaba, K., ・lzumi, H., ・Nomura, M. ・Murata, M.
  • Journal Title: Front. BioSci (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans (2008)
  • Author(s): Yoshida K., Kawano N., Yoshiike M., Yoshida M., Iwamoto T. and Morisawa M.
  • Journal Title: Human Reproduction 14 Pages: 151-156
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Adaptation and strategy for fertilization in the sperm of teleost fishes (2008)
  • Author(s): Morisawa M.
  • Journal Title: J. Appl. Ichthyol. 24 Pages: 362-370
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of two egg-derived molecules in motility initiation and fertilization in the Pacific herring(Clupea pallasi) (2008)
  • Author(s): Cherr GN., Morisawa M., Vines CA., Yoshida K., Smith EH. Matsubara.T., PillaiMC., Griffin, FJ., and Yanagimachi R.
  • Journal Title: J. Develop. Biol. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] activation and chemotaxis in ascidian sperm (2008)
  • Author(s): Morisawa, M., Yoshida, M., Inaba, K., Izumi, H., Nomura, M. and Murata, M. Motility
  • Journal Title: Front. BioSci. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sperm-derived motility initiating substance from amphioxus Branchiostom belcheri. (2006)
  • Author(s): Tanaka, H., ・Kubokawa, K., ・Mosisawa, M.
  • Journal Title: J. Exp. Zool. 305A Pages: 68-73
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of the Na-/Ca^<2+> exchanger in calcium homeostasis and human sperm motility regulation. (2006)
  • Author(s): Krasanai, Z., ・Krasznai, ZT., ・Morisawa, M., ・Bazsanekassai, Zs., ・Hernadi, Z., ・Fazekas, ZS., ・Tron, L., ・Goda, K., ・Marian, T.
  • Journal Title: Cell Motil. Cytoskelt. 63 Pages: 66-76
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Na+/Ca^<2+> exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa. (2006)
  • Author(s): Shiba, K., ・Marian, T., ・Krasznai, Z., ・Baba, SA., <Morisawa, M.・Yoshida, M.
  • Journal Title: Cell Motile, Cytoskelt., 63 Pages: 623-632
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Sperm-derived sperm motility initiating substance from amphioxus Branchiostom belcheri (2006)
  • Author(s): Tanaka, H., Kubokawa, K., and Morisawa. M.
  • Journal Title: J. Exp. Zool. 305A Pages: 68-73
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of the Na^<+>/Ca^<2+> exchanger in calcium homeostasis and human sperm motility regulation (2006)
  • Author(s): Krasznai, Z., Krasznai, ZT, Morisawa, M., Bazsane Kassai, ZS., Hernadi, Z., Fazekas, ZS., Tron, L., Goda, K., Marian, T.
  • Journal Title: Cell Motil. Cytoskel. 63 Pages: 66-76
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Na^<+>/Ca2+ exchanger modulates the flagellarwave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa (2006)
  • Author(s): Shiba, K., Marian, T., Krasznai, Z., Baba, SA., Morisawa, M. and Yoshida, M.
  • Journal Title: Cell Motile, Cytoskel 63 Pages: 623-632
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sperm-derived sperm motility initiating substance from amphioxus Branchiostom belcheri (2006)
  • Author(s): Tanaka, H., Kubokawa, K., Morisawa,M
  • Journal Title: Jounal of Experimental Zoology 305A:
• [Journal Article] Role of the Na+/Ca2+exchanger in calciumhomeostasis and human sperm motility regulation. (2006)
  • Author(s): Krasznai Z, Krasznai ZT, Morisawa M, Bazsane Kassai Zs, Henade Z, Fazekas Zs, Tron L, Goda K, Marian T
  • Journal Title: Cell Motility and Cytoskeleton 63 Pages: 66-76
• [Journal Article] Na+/CaII+exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa. (2006)
  • Author(s): Shiba, K., Marian, T., Krasznai, Z, Baba, SA., Morisawa, M., Yoshida,M.
  • Journal Title: Cell Motility and Cytoskeleton 63 Pages: 623-632
• [Journal Article] The outermost layer of egg-jelly if crucial to successful fertilization in the newt,cynops pyrrhogaster (2006)
  • Author(s): Takahasi, S., Nakazawa, H., Watanabe, A., K.Onitake
  • Journal Title: Journal of experimental Zoology 305A Pages: 1010-1017
• [Journal Article] 精子の活性化・走化性機構 (2006)
  • Author(s): 森澤正昭, 吉田 学
  • Journal Title: 新編精子学 Pages: 94-115
• [Journal Article] Role of two egg-derived molecules in motility initiation and fertilization in the Pacific herring (Clupea Pallasi)
  • Author(s): Cherr, GN, Morisawa, M, Vines, CA., Yoshida, K., Smith, EH, Matsubara, T. pillai, MC, Griffin, FJ. and Yanagimachi, R
  • Journal Title: Jounal Developmental. Biology (印刷中)
  • Peer Reviewed
• [Journal Article] Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans
  • Author(s): Yoshida, K, kawano, N, Yoshiik, M, Yoshida, M, Iwamoto, T, Morisawa, M
  • Journal Title: Human Reproduction (印刷中)
  • Peer Reviewed
• [Journal Article] Adaptation and strategy for fertilization in the sperm of teleost fishes. Reproduction in press(2007).
  • Author(s): Morisawa, M.,
  • Journal Title: Jounal applied Ichthyology (印刷中)
  • Peer Reviewed
• [Book] 新編精子学 (2006)
  • Author(s): 毛利秀雄, 星 元紀監修, 森澤正昭, 星 和彦, 岡部 勝編
  • Total Pages: 489
  • Publisher: 東京大学出版会