| Budget Amount *help |
¥4,080,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
During this project studying the biodiversity of the basidiomycetous fungi that habitat members of the mangrove trees, we collected their 90 specimens and 34 isolates from the mangrove forests of the islands of Okinawa, Ishigaki, Iriomote and Amami-oshima, located in the Ryukyu Islands of Japan. In the macro- and micromorphological observation of the specimens, 47 species of 30 genera, including a new species of Asterostroma, were identified. Analysis of the basidiomycetous species identified in this study reveals that 71% of the total number of the species belongs to the corticioid fungi (Corticiaceae sensu lab)) and that Auricularia polytricha, Heterochaete delicata, Hyphodontia ovispora, Phlebia acanthocystis and Tremellochaete japonica appear to be common species in the Japanese mangroves. In the ribosomal DNA analysis of the isolates obtained from the decaying wood of mangroves, at least the other 7 species different from the above 47 species were recognized although their basidiomata did not observed in the field. Optimal temperature for the most basidiomycetous species obtained from mangroves was 27-30℃. In the test for salt tolerance, mycelia of Haloaleurodiscus mangroves, Phellinus mangrovicus and Xylobolus sp. were able to grow even though slowly on MEA containing higher concentrations of sea salts than those (32-35‰) of seawater, although their growth was inferior in the comparison of that on freshwater agar media. These cultural features lead us to conclude that the three species are salt tolerant, and also suggest that it is possible to produce the basidiomata on the substrate periodically covered by the tide during their development.
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