Molecular mechanisms of morphogenesis and response to environmental cues in cladoceran crustaceans

• Project/Area Number: 18570093
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: YAMAGATA Hideo Tokyo University of Pharmacy and Life Science, School of Life Sciences, Professor (20023468)
• Co-Investigator(Kenkyū-buntansha): HAYASEII Shigeo RIKEN Center for Developmental Biology, Morphogentic Signaling Group, Director (60183092) ; HANAZATO Takayuki Shinshu University, Research and Education Center for InlandwaterEnvironment, Professor (60142105)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: plankton / ecology / gene / development and differentiation / reproduction cycle / 生物圏現象 / 多様性 / 発生・文化

Research Abstract

Abundant expression of transcriptional read through products of two adjacent Hox genes, Ubx and Antp, was found in five crustacean species of Branchiopoda and Malacostaraca. In Daphnia, bicistronic Ubx/Antp mRNA produced no significant level of either Ubx or ANTP. In Artemia, on the other hand, the bicistronic mRNA produced only Ubx, and replaced the role of monocistronic Ubx mRNA. In this way, multiple post-transcriptional control mechanic ms in two extant branchiopodans can be seen as preventing the potentially deleterious consequences of fox gene fusion.
Expression of D. magna aryl hydrocarbon receptor nuclear translocator (ARNT) in ARNT-lacking mouse Hepa-c4 cells resulted in the compensation for the loss of hypoxia response. D. magna ARNT was expressed in various tissues at the middle to late stages of embryonic development. In contrast, expression of ARNT in adult daphnids was limited to the epipodites of thoracic limbs, suggesting that ARNT plays a role solely in hypoxia response. Juvenile hormone (JH) agonists, pyriproxyfen and fenoxycarb, strongly repressed the expression of vitellogenin genes in neonate daphnids. The intergenic region of 2.6 kb between the D. magna vitellogenin genes contained sequences resembling known JH-responsive and ecdysone-responsive elements. A D. magna cDNA homolog of CPEB, a sex-determinant in C. elegans, was isolated and characterized. Amount of CPEB mRNA was decreased to one seventh after exposure of daphnids to JH. CPEB mRNA was expressed specifically in germ line cells in daphnids at the stages of yolk formation. Subunit composition of hemoglobins induced by exposure of daphnids to JH was different from that of hemoglobins induced by hypoxia.

Research Products

• [Journal Article] From critters to cancers: bridging comparative and clinical research on oxygen sensing, HIF signaling, and adaptations towards hypoxia (2007)
  • Author(s): Hoogewijs, D., Tokishita, S., Yamagata, H., Gorr, T, A., et. al.
  • Journal Title: Integr.Comp.Biol. 47 Pages: 552-577
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Organization and repression by juvenile hormone of a vitellogenin gene cluster in the crustacean, Daphnia magna (2007)
  • Author(s): Tokishita, S., Kato, Y., Kobayashi, T., Nakamura, S., Ohta, T., Yamagata, H
  • Journal Title: Biochemical and Biophyscal Researchh Communications 345 Pages: 362-370
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] From critters to cancers : bridging comparative and clinical research on oxygen sensing, HIF signaling, and adaptations towards hypoxia (2007)
  • Author(s): Hoogewijs, D., Tokishita, S., Yamagata, H., Gorr, T, A., et. al.
  • Journal Title: Integral and Comparative Biology 47 Pages: 552-577
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] From critters to cancers: bridging comparative and clinical research on oxygen sensing, HIF signalling, and adaptations towards hypoxia (2007)
  • Author(s): D. Hoogewijs, H. Yamagata, et. al.
  • Journal Title: Integrative and Comparative Biology 47 47 Pages: 552-557
  • Peer Reviewed
• [Journal Article] Organization and repression by juvenile hormone of a vitellogenin gene cluster in the crustacean,Daphnia magna (2006)
  • Author(s): Tokishita, S., Kato, Y., Kobayashi, T., Nakamura, S., Ohta, T., Yamagata, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 345 Pages: 362-370
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Tissue-specific expression of a bHLH-PAS protein homologous to ARNT during the development of crustacean Daphnia magna (2006)
  • Author(s): Tokishita, S., Kimura, S., Mandokoro, Y., Kato, K., Shiga, Y., Takahashi, Y., Ohta, T., Yamagata, H.
  • Journal Title: Gene 376 Pages: 231-239
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transcriptional readthrough of Hox genes Ubx and Mtp and their divergent post-transcriptional control during crustacean evolution (2006)
  • Author(s): Shiga, Y., Sagawa, K., Takai, R., Sakaguchi, H., Yamagata, H.
  • Journal Title: Evol.Dev. 8 Pages: 407-414
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transcriptional readthrough of Hox genes Ubx and Antp and their divergent post-transcriptional control during crustacean evolution (2006)
  • Author(s): Shiga, Y., Sagawa, K., Takai, R., Sakaguchi, H., Yamagata, H
  • Journal Title: Evoltion and Development 8-5 Pages: 407-414
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tissue-specific expression of a bHLH-PAS protein homologous to ARNT during the development of crustacean Daphnia magna (2006)
  • Author(s): Tokishita, S., Kimura, S., Mandokoro, Y., Kato, K., Shiga, Y., Takahashi, Y., Ohta, T., Yamagata, H
  • Journal Title: Gene 376 Pages: 231-239
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Organization and repression by juvenile hormone of a vitellogenin gene cluster in the crustacean, Daphnia magna. Organization and repression by juvenile hormone of a vitellogenin gene cluster in the crustacean, Daphnia magna. (2006)
  • Author(s): Tokishita, S, Kato, Y, Kobayashi, T, Nakamura, S, Ohta, T, Yamagata, H
  • Journal Title: Biochem. Biophys. Res. Commun. 345 Pages: 362-370
• [Journal Article] Tissue-specific expression of a bHLH-PAS protein homologous to ARNT during the development of crustacean Daphnia magna. (2006)
  • Author(s): Tokishita, S, Kimura, S, Mandokoro, Y, Kato, K, Shiga, Y, Takahashi, T, Ohta, T, Yamagata, H
  • Journal Title: Gene 376 Pages: 231-239
• [Journal Article] Transcriptional readthrough of Hox genes Ubx and their divergent post-transcriptional control during crustacean evolution. (2006)
  • Author(s): Shiga, Y, Sagawa, K, Takai, R, Sakaguchi, H, Yamagata, H, Hayashi, S
  • Journal Title: Evol. Dev 8 Pages: 407-414
• [Presentation] 白樺湖におけるカブトミジンコの遺伝的解析 (2008)
  • Author(s): 渋谷浩之、花里孝幸、山形秀夫等
  • Organizer: 日本生態学会第55回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-03-17
• [Presentation] オオミジンコBouleホモログの単離と解析 (2007)
  • Author(s): 時下進一、川城知子、山形秀夫等
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] オオミジンコCPEB1遺伝子群の解析 (2007)
  • Author(s): 米倉温、時下進一、山形秀夫等
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] Expression of HIF and hypoxic induction of hemoglobin in the crustacean Daphnia magna (2006)
  • Author(s): Tokishita, S., Yamagata, H.
  • Organizer: The lst International Congress of Respiratory Biology
  • Place of Presentation: Bonn,Germany
  • Year and Date: 2006-08-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression of HIF and hypoxic induction of hemoglobin in the crustacean Daphnia magna (2006)
  • Author(s): Tokishita, S., Yamagata, H
  • Organizer: The 1st International Congress of Respiratory Biology
  • Place of Presentation: Bonn, Germany
  • Year and Date: 2006-08-15
  • Description: 「研究成果報告書概要(欧文)」より