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Analysis of the molecular mechanism underlying the maintenance of endosymbiotic system in aphid

Research Project

Project/Area Number 18570121
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

MORIOKA Mizue  The University of Tokyo, Graduate School of Science,, Assistant Professor (20272461)

Co-Investigator(Kenkyū-buntansha) SASAKI Tetsuhiko  Tamagawa University, Research Institute, Associate Professor (60235257)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsSymbiosis / Aphid / Buchnera / Lysosome / CPVL / 細胞内共生 / 鞭毛フック基部(HBB) / タンパク質輸送 / 膜輸送系
Research Abstract

Endosymbiotic system in the aphid is maintained through a mutualistic association between the host and a symbiotic bacterium, Buchnera, which is harbored in particular host cell called bacteriocyte. In the present study, we demonstrated the dynamics of Buchnera-aphid symbiosis, and following results were obtained.
1) Buchnera density in bacteriocytes fluctuated according to the life cycle of the host in an age- and morph-dependent manner. Buchnera density decreased drastically just around the final ecdysis in the winged morph alatae, when the indirect flight muscles develop, whereas it decreased at the early larviposition stage in the wingless morph apterae.
2) Proteomic analysis of the bacteriocyte p roteins just after the final ecdysis revealed the enhanced expression of carboxypeptidase-vitellogenic like (CPVL), in the alatae bacteriocytes compared to the apterae bacteriocytes. Immunoblotting analysis using anti-CPVL antiserum revealed that Aphid-CPVL is activated in the alatae bacteriocytes accompanying with the processing, and functions as a lysosomal enzyme to degrade the Buchnera.
3) Histochemical analysis using LysoTracker indicated that the lysosomal system is drastically activated in the alatae bacteriocytes just after the final ecdysis. Further, electron microscope observations revealed many degrading Buchnera that contained irregularly spaced electron-dense areas and were enclosed by a distended symbiosome membrane in the alatae bacteriocytes.
These findings suggest that the host lysosomal system is involved in age-and morph-dependent regulation of Buchnera density in the aphid symbiotic system and the degraded Buchnera may be supplied as nutrients to the host. We considered that the result obtained in the present study would contribute in elucidating the molecular mechanism underlying the maintenance of endosymbiotic system in aphid.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (11 results)

All 2008 2007 2006 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Involvement of host lysosome in the regulation mechanism of Buchnera Density in aphid endosymbiotic sustem2008

    • Author(s)
      錦織健児, 森岡清和, 久保健雄, 森岡瑞枝
    • Journal Title

      Proc. Natl. Acad. Sci. USA (査読中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hundreds of Flagellar Basel Bodies Cover the Cell Surface of the Endosymbiotic Bacterium Buchnera aphidicola sp. Strain APS.2006

    • Author(s)
      Kazuki Maezawa
    • Journal Title

      Journal of Bacteriology 188・18

      Pages: 6539-6543

    • Related Report
      2006 Annual Research Report
  • [Presentation] アブラムシ細胞内共生系におけるブフネラ密度の変動とその制御機構の解析2007

    • Author(s)
      錦織健児、久保健雄、森岡瑞枝
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Analysis of the molecular mechanism of decrease of Buchnera density in aphid symbiotic system2007

    • Author(s)
      Nishikori, K., Kubo, T., and Morioka, M.
    • Organizer
      BMB2007 : The 30th Annual Meeting of the Molecular Biology Society of Japan and The 80th Annual Meeting of the Japanese Biochemical Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アブラムシ細胞内共生系におけるブフネラ密度制御機構2007

    • Author(s)
      錦織健児、久保健雄、森岡瑞枝
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Regulation of Buchnera density in endosymbiotic system of aphid2007

    • Author(s)
      Nishikori, K., Kubo, T., and Morioka, M.
    • Organizer
      The 78th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Hirosaki
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Morphological variability of endosymbiotic bacterium, Buchnera, in bacteriocyte depending on the morph of the host insect2006

    • Author(s)
      錦織健児、久保健雄、森岡瑞枝
    • Organizer
      第7回日米合同組織細胞化学会議
    • Place of Presentation
      ハワイ
    • Year and Date
      2006-08-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Morphological variability of symbiotic bacterium, Buchnera, in bacteriocyte depending on the morph of the host insect2006

    • Author(s)
      Nishikori, K., Kubo, T., and Morioka, M.
    • Organizer
      The 7th Joint Meeting of the Japan Society of Histochemistry and cytochemistry and the US Histochemical Society
    • Place of Presentation
      Hawaii
    • Year and Date
      2006-06-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Temporal disintegration of mutuarity in aphid endosymbiosis2006

    • Author(s)
      錦織健児、久保健雄、森岡瑞枝
    • Organizer
      第20回国際生化学・分子生物学会議
    • Place of Presentation
      京都
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Temporal disintegration of mutuality in aphid endosymbiosis2006

    • Author(s)
      Nishikori, K., Kubo, T., and Morioka, M.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://kubo.biol.s.u-tokyo.ac.jp/?dest=disporder

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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