Possible function of IP3 receptor as a non-channel molecule

• Project/Area Number: 18570122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The Institute of Physical and Chemical Research (2008); The University of Tokyo (2006-2007)
• Principal Investigator: MIZUTANI Akihiro The Institute of Physical and Chemical Research, 発生神経生物研究チーム, 研究員 (30242861)
• Co-Investigator(Kenkyū-buntansha): HATANO Naoya 神戸大学, 医学研究所, グロバールCOE研究員 (10332280)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: IP3レセプター / IRBIT / 小脳 / シナプス可塑性 / リン酸化

Research Abstract

IP3レセプターに結合し、IP3によって放出されるIRBIT分子のIP3レセプターへの結合に必須な4カ所のリン酸化部位同定に成功した。さらに、IRBITに結合する種々の機能分子の同定にも成功し、IP3レセプター-IRBIT-IRBIT結合蛋白質群による、細胞機能調節機構の存在を明らかにした。

Research Products

• [Journal Article] IRBIT coordinates epithelial fluid and HCO3- secretion by stimulating the transporters pNBC1 and CFTR in the murine pancreatic duct (2009)
  • Author(s): Yang D, Shcheynikov N, Zeng W, Ohana E, So I, Ando H, Mizutani A, Mikoshiba K, Muallem S.
  • Journal Title: J Clin Invest. 119 Pages: 193-202
  • Peer Reviewed
• [Journal Article] 80K-H Interacts with Inositol 1,4,5-Trisphosphate (IP3) Receptors and Regulates IP3-induced Calcium Release Activity (2009)
  • Author(s): Kawaai K
  • Journal Title: J. Biol. Chem. 284 Pages: 372-380
  • Peer Reviewed
• [Journal Article] Phosphorylation of Homer3 by calcium/calmodulin-dependent kinase II regulates a coupling state of its target molecules in Purkinje cells (2008)
  • Author(s): Mizutani A, Kuroda Y, Futatsugi A, Furuichi T, Mikoshiba K.
  • Journal Title: J. Neurosci. 28 Pages: 5369-6382
  • Peer Reviewed
• [Journal Article] Phosphorylation of Homer3 by calcium/calmodulin-dependent kinase II regulates a coupling state of its target molecules in Purkinje cells (2008)
  • Author(s): A. Mizutani
  • Journal Title: J. Neuroscience 28 Pages: 5369-6382
  • Peer Reviewed
• [Journal Article] The role of Ca2+ signaling in cell function with special reference to exocrine secretion (2008)
  • Author(s): Mikoshiba K
  • Journal Title: Cornea 27
• [Journal Article] Phosphorylation of Homer3 by CaMKII regulates a coupling state of its target molecules in Purkinje Cells. (2008)
  • Author(s): Mizutani, A, et. al.
  • Journal Title: J. Neuroscience (掲載確定)
  • Peer Reviewed
• [Journal Article] Activation of SAD kinase by Ca2+/calmodulin-dependent protein kinase kinase. (2008)
  • Author(s): Fujimoto, T, et. al.
  • Journal Title: Biochemsitry 47 Pages: 4151-4159
  • Peer Reviewed
• [Journal Article] Predominant role of type 1 IP3 receptor in aortic vascular muscle contraction. (2008)
  • Author(s): Zhou, H, et. al.
  • Journal Title: Biochem Biophys Res Commun. 369 Pages: 213-219
  • Peer Reviewed
• [Journal Article] 2-Aminoethyl diphenylborinate analogues: Selective inhibition for store-operated Ca^<2+> entry (2007)
  • Author(s): Zhou H, Iwasaki H, Nakamura T, Nakamura K, Maruyama T, Hamano S, Ozaki S, Mizutani A, Mikoshiba K.
  • Journal Title: Biochem. Biophys. Res.Commun. 352 Pages: 277-282
  • Peer Reviewed
• [Journal Article] IRBIT suppresses IP3 receptor activity by competing with IP3 for the common binding site on the IP3 receptor in a phosphorylation-dependent manner (2006)
  • Author(s): Ando H, Mizutani A, Kiefer H, Tsuzurugi D, Michikawa T, & Mikoshiba K.
  • Journal Title: Mol. Cell 22 Pages: 795-806
  • Peer Reviewed
• [Journal Article] IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/HCO3-cotransporter 1 (pNBC1). (2006)
  • Author(s): Shirakabe K., et al.
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. 103・25 Pages: 9542-9547
• [Journal Article] IRBIT suppresses IP3 receptor activity by competing with IP3 for the common binding site on the IP3 receptor. (2006)
  • Author(s): Ando H., et al.
  • Journal Title: Mol. Cell 22・6 Pages: 795-806
• [Journal Article] The role of Ca2+ signaling in cell function with special reference to exocrine secretion (2000)
  • Author(s): Mikoshiba K, Hisatsune C, Futatsugi A, Mizutani A, Nakamura T, Miyachi K.
  • Journal Title: Cornea 27, Suppl 1
  • Peer Reviewed
• [Presentation] Purkinje細胞におけるHomer3 のリン酸化 (2008)
  • Author(s): 水谷 顕洋、黒田有希子、古市 貞一、御子柴克彦
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Patent(Industrial Property Rights)] IP3受容体結合タンパク質による細胞内標的分子の制御 (2007)
  • Inventor(s): 御子柴克彦、水谷顕洋、安東英明
  • Industrial Property Rights Holder: 独立行政法人理化学研究所・独立行政法人科学技術振興機構
  • Industrial Property Number: 2007-073396
  • Filing Date: 2007-03-20