Study on the expression and function of sex pheromone in ciliates

• Project/Area Number: 18570129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Nara Women's University
• Principal Investigator: HARUMOTO Terue Nara Women's University, 理学部, 教授 (80198936)
• Co-Investigator(Kenkyū-buntansha): SUZAKI Toshinobu 神戸大学, 大学院・理学研究科, 准教授 (00187692) ; IIO Hideo 大阪市立大学, 大学院・理学研究科, 教授 (80145771)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,070,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: ホルモンと生理活性物質 / 繊毛虫 / 接合 / タンパク質 / 発現調節 / 糖鎖 / 受容体 / 糖タンパク質 / 大量発現系 / rRNA遺伝子 / 種間関係 / cdk / cks / 4-HPPD / 原生動物 / 相同遺伝子 / 接合型 / 性フェロモン / ブレファリズマ / 有性生殖 / 遺伝子 / PCR

Research Abstract

原生生物繊毛虫Blepharismaの接合誘導時特異的に発現する遺伝子を単離・同定した。糖鎖を欠く接合誘導物質ガモン1を(1) 酵素処理、および(2) 大腸菌を用いた大量発現系により得て、ガモン1の糖鎖は誘導活性に重要な役割をもつことを明らかにした。ガモン1に対する抗体を作製し、II型細胞表面にあるガモン1受容体の探索を行った。野外で採集したBlepharismaの種を同定し、rRNA遺伝子とガモン1遺伝子の配列を調べ、Blepharismaの種の分岐について考察した。

Research Products

• [Journal Article] Investigation of amino acid residues of eRF1 which are responsible for stop codon recognition in ciliates (2009)
  • Author(s): Lee Y, Kim OTP and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 42 Pages: 21-22
• [Journal Article] Analysis of gamone 1 receptor in the ciliate Blepharisma japonicum using a gamone 1 antibody (2009)
  • Author(s): Sugiura M, Harumoto T and Suzaki T
  • Journal Title: Japanese Journal of Protozoology 42 Pages: 34-35
• [Journal Article] 繊毛虫Blepharisma japonicumにおける接合誘導物質ガモン1の糖鎖の役割 (2009)
  • Author(s): Iwasaki S, Sugiura M and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 42 Pages: 76-77
• [Journal Article] タイヨウチュウRaphidiophrys contractilisを用いた水質モニタリングシステムに応用可能な簡易型流動チャンバーの検討 (2009)
  • Author(s): 榎本淑恵, 福泉翔, 枝松緑, 洲崎敏伸, 安藤元紀
  • Journal Title: 原生動物学雑誌 42 Pages: 22-23
• [Journal Article] ミドリゾウリムシの細胞内共生クロレラは共生することで細胞壁が変化する (2009)
  • Author(s): 西垣貴美子, 松元里樹, 矢頭卓児, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 42 Pages: 44-46
• [Journal Article] Euglena gracilisの2種類のIP39タンパク質のペプチド抗体を用いた解析 (2009)
  • Author(s): 竹内喬平, 末友靖隆, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 42 Pages: 40-41
• [Journal Article] ペラネマPeranema trichophorumにおけるマスティゴネマの単離と構成タンパク質の分析 (2009)
  • Author(s): 吉見英明, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 42 Pages: 75-76
• [Journal Article] ミドリゾウリムシへのイーストPichia capsulataの細胞内共生 (2009)
  • Author(s): ソンチホン, 松元里樹, 西垣貴美子, 矢頭卓児, 島田晴美, 大村現, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 42 Pages: 37-39
• [Journal Article] ミドリゾウリムシにおける宿主-共生クロレラ間でのアミノ酸輸送に関する研究 (2009)
  • Author(s): 加藤豊, 木下宗, 大村現, 洲崎敏伸, 今村信孝
  • Journal Title: 原生動物学雑誌 42 Pages: 86-88
• [Journal Article] 祖先的渦鞭毛虫の核タンパク質と染色体 (2009)
  • Author(s): 福田康弘, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 42 Pages: 46-47
• [Journal Article] Investigation of amino acid residues of eRF1 which are responsible for stop codon recognition in ciliates (2009)
  • Author(s): Lee Y, Kim OTP, Harumoto T
  • Journal Title: Japanese Journal of Protozoology 42 Pages: 21-22
• [Journal Article] Analysis of gamone 1 receptor in the ciliate Blepharisma japonicum using a gamone 1 antibody (2009)
  • Author(s): Sugiura M, Harumoto T, Suzaki T
  • Journal Title: Japanese Journal of Protozoology 42 Pages: 34-35
• [Journal Article] Role of the oligosaccharide attached to gamone 1 in the conjugation-inducing activity of the ciliate in Blepharisma japonicum (2009)
  • Author(s): Iwasaki S, Sugiura M, Harumoto T
  • Journal Title: Japanese Journal of Protozoology 42 Pages: 76-77
• [Journal Article] Ciliates use both variant and universal genetic codes : evidence of omnipotent eRF1s in the class Litostomatea (2008)
  • Author(s): Kim, OTP, Sakurai, A, Saito, K, Ito, K, Ikehara, K and Harumoto, T
  • Journal Title: Gene 417 Pages: 51-58
  • Peer Reviewed
• [Journal Article] Molecular features of the cdk1 and cks gene homologs expressed specifically during induction of conjugation in mating type II cells of Blepharisma japonicum (2008)
  • Author(s): Tanaka Y, Sugiura M and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 42-44
• [Journal Article] The relation between the stop codon reassignment and the specificity of eukaryotic release factor (eRF1) in Blepharisma (2008)
  • Author(s): Kim OTP, Ikehara K and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 44-46
• [Journal Article] Is the oligosaccharide attached to gamone 1 in the ciliate Blepharisma japonicum essential for conjugation-inducing activity? (2008)
  • Author(s): Iwasaki S, Sugiura M and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 85-86
• [Journal Article] Initiation of gamone 1 transcription by sudden deprivation in Blepharisma japonicum (2008)
  • Author(s): Yamanaka M, Sugiura M, Tanaka Y and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 86-88
• [Journal Article] Ca^<2+>-and glycoconjugates-dependent prey capture in the heliozoon Actinophrys sol (2008)
  • Author(s): Kakuta, S. and Suzaki, T
  • Journal Title: Eur. J. Protistol 44 Pages: 163-167
  • NAID: 120001546360
• [Journal Article] ユーグレナ類Euglena gracilisの膜内在性タンパク質IP39は2タイプ存在する (2008)
  • Author(s): 末友靖隆, 竹内恭平, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 41 Pages: 58-60
• [Journal Article] 基底面への接着力を指標としたタイヨウチュウ水質モニタリング装置の改良 (2008)
  • Author(s): 吉村知里, 大村現, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 41 Pages: 57-58
• [Journal Article] ミドリゾウリムシのPV膜タンパク質のSDS-PAGE解析 (2008)
  • Author(s): 平岡三和, 大村現, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 41 Pages: 93-94
• [Journal Article] 原生動物細胞の形態的・生理学的特性をモニターするための誘電解析プログラムの開発 (2008)
  • Author(s): 洲崎敏伸
  • Journal Title: 原生動物学雑誌 41 Pages: 54-55
• [Journal Article] 誘電解析法を用いたミドリムシの細胞変形能の解析, 原生動物学雑誌 (2008)
  • Author(s): 福泉翔, 枝松緑, 洲崎敏伸, 安藤元紀
  • Journal Title: 原生動物学雑誌 41 Pages: 55-57
• [Journal Article] Implications of microbial symbionts on defense and competition of hosts-ciliates (2008)
  • Author(s): Gortz, H. -D., Rosati, G., Schweikert, M., Schrallhammer, M., Omura, G. and Suzaki, T.
  • Journal Title: "Defensive Mutalism in Microbial Symbiosis" (ed. : Jim White and Monica Torres) (in press)
  • Peer Reviewed
• [Journal Article] 連続誘電測定によるミドリムシの細胞動態の解析 (2008)
  • Author(s): 福泉翔、榎本淑恵、枝松緑、洲崎敏伸、安藤元紀
  • Journal Title: 原生動物学雑誌 42 Pages: 74-74
• [Journal Article] Starter units of the biosynthesis of blepharismins : self-defense pigments of Blepharisma japonicum (2008)
  • Author(s): Yoshioka, K., Tominaga, S., Uruma, Y., Usuki, Y., Iio, H.
  • Journal Title: Tetrahedron 64 Pages: 4104-4107
  • Peer Reviewed
• [Journal Article] The protozoan toxin climacostol inhibits growth and induces apoptosis of human tumor cell lines (2008)
  • Author(s): F. Buonanno, L. Quassinti, M. Bramucci, C. Amantini, R. Lucciarini, G. Santoni, H. Iio, C. Ortenzi
  • Journal Title: Chemico-Biological Interactions 176 Pages: 151-164
  • Peer Reviewed
• [Journal Article] Ciliates use both variant and universal genetic codes : evidence of omnipotent eRF1s in the class Litostomatea (2008)
  • Author(s): Kim, OTP, Sakurai, A, Saito, K, Ito, K, Ikehara, K, Harumoto, T
  • Journal Title: Gene 417 Pages: 51-58
  • Peer Reviewed
• [Journal Article] Molecular features of the cdkl and cks gene homologs expressed specifically during induction of conjugation in mating type II cells of Blepharisma japonicum (2008)
  • Author(s): Tanaka Y, Sugiura M, Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 42-44
• [Journal Article] The relation between the stop codon reassignment and the specificity of eukaryotic release factor (eRF1)in Blepharisma (2008)
  • Author(s): Kim OTP, Ikehara K, Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 44-46
• [Journal Article] Is the oligosaccharide attached to gamone 1 in the ciliate Blepharisma japonicum essential for conjugation-inducing activity? (2008)
  • Author(s): Iwasaki S, Sugiura M, Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 85-86
• [Journal Article] Initiation of gamone 1 transcription by sudden food deprivation in Blepharisma japonicum (2008)
  • Author(s): Yamanaka M, Sugiura M, Tanaka Y, Harumoto T
  • Journal Title: Japanese Journal of Protozoology 41 Pages: 86-88
• [Journal Article] Ciliates use both variant and universal genetic codes: evidence of omnipotent eRF1s in the class Litostomatea (2008)
  • Author(s): Kim, O. T. P., Sakurai, A., Saito, K., Ito, K., Ikehara, K. and Harumoto, T.
  • Journal Title: Gene DOI link: http://dx.doi.org/10.1016/j.gene.2008.03.018 (In press)
  • Peer Reviewed
• [Journal Article] Cdk1 and cks gene homologs are transcriptionally activated during induction of conjugating pairs in mating-type II cells of the ciliate Blepharisma japonicum (2007)
  • Author(s): Tanaka Y, Sugiura M and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 131-138
  • NAID: 130006070179
  • Peer Reviewed
• [Journal Article] Synthesis of fluorescent molecular probes specific for the receptor of blepharismone, a mating-inducing pheromone of the ciliate Blepharisma japonicum (2007)
  • Author(s): Uruma Y, Sugiura, M, Harumoto, T, Usuki Y and Iio H
  • Journal Title: Bioorganic & Medicinal Chemistry 15 Pages: 1622-1627
  • Peer Reviewed
• [Journal Article] Bioconvection and front formation of Paramecium tetraurelia (2007)
  • Author(s): Kitsunezaki S, Komori R and Harumoto T
  • Journal Title: Physical Review E (046301) 76 Pages: 1-10
  • Peer Reviewed
• [Journal Article] How is ciliate eukaryotic release factor 1 (eRF1) different from conventional eRF1s? -In vivo complementary activity of Dileptus eRF1- (2007)
  • Author(s): Kim OTP, Ikehara K & Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 52-53
• [Journal Article] Identification of gamone 1 gene homolog and determination of the sequence in the ciliate Blepharisma stoltei (2007)
  • Author(s): Miura M, Sugiura M & Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 66-67
  • NAID: 40015440040
• [Journal Article] In vivo complementation test of Didinium nasutum eRF1 in yeast for understanding stop codon usage in this species (2007)
  • Author(s): Sakurai A, Kim OTP & Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 70-71
• [Journal Article] Phytophthora nicotianaeのマスティゴネマ構成タンパク質14B7の遺伝子解析 (2007)
  • Author(s): 山田周平, 有川幹彦, Blackmann, L.M., Hardham, A.R., 洲崎敏伸
  • Journal Title: 原生動物学雑誌 40 Pages: 50-51
• [Journal Article] 太陽虫Actinophrys solにおけるグルカン結合タンパク質gp40のcDNAクローニング (2007)
  • Author(s): 角田宗一郎, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 40 Pages: 55-56
• [Journal Article] Paramecium bursariaからのPV膜付き共生クロレラの単離と透過型電子顕微鏡観察 (2007)
  • Author(s): 大村現, 平岡三和, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 40 Pages: 76-78
• [Journal Article] Assignment of 13C NMR spectrum for blepharismin C based on biosynthetic studies (2007)
  • Author(s): Uruma, Y. ; Sakamoto, K. ; Takumi, K. ; Doe, M. ; Usuki, Y. ; Iio, H.
  • Journal Title: Tetrahedron 63 Pages: 5548-5553
  • Peer Reviewed
• [Journal Article] Cdk1 and cks gene homologs are transcriptionally activated during induction of conjugating pairs in mating-type II cells of the ciliate Blepharisma japonicum. (2007)
  • Author(s): Tanaka Y, Sugiura M and Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 131-138
  • NAID: 130006070179
  • Peer Reviewed
• [Journal Article] Synthesis of fluorescent molecular probes specific for the receptor of blepharismone, a mating-inducing pheromone of the ciliate Blepharisma japonicum. (2007)
  • Author(s): Uruma Y, Sugitua, M, Harumoto, T, Usuki Y and Iio H
  • Journal Title: Bioorganic & Medicinal Chemistry 15 Pages: 1622-1627
  • Peer Reviewed
• [Journal Article] Bioconvection and fiont formation of Paramecium tetraurelia. (2007)
  • Author(s): Kitsunezaki S, Komori R and Harumoto T
  • Journal Title: Physical Review E 76:046301 Pages: 1-10
  • Peer Reviewed
• [Journal Article] How is ciliate eukaryotic release factor 1(eRF1) different from conventional eRF1s?-In vivo complementary activity of Dileptus eRF1 (2007)
  • Author(s): Kim OTP, Ikehara K & Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 52-53
• [Journal Article] Identification of gamone 1 gene homolog and determination of the sequence in the ciliate Blepharisma stoltei, (2007)
  • Author(s): Miura M, Sugiura M & Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 66-67
  • NAID: 40015440040
• [Journal Article] n vivo complementation test of Didinium nasutum eRF1 in yeast for understanding stop codon usage in this species (2007)
  • Author(s): Sakurai A, Kim OTP & Harumoto T
  • Journal Title: Japanese Journal of Protozoology 40 Pages: 70-71
• [Journal Article] Photodynamic killing of Blepharisma (albino), Climacostomum and Dileptus by treatment with photosensitizer pigments (2006)
  • Author(s): 寺嶋昌代、春本晃江
  • Journal Title: Japanese Journal of Protozoology Vol.39(1) Pages: 121-123
• [Journal Article] Identification of a set of genes expressed during induction of conjugation in the ciliate Blepharisma japonicum (2006)
  • Author(s): 杉浦真由美、田中悠里、久保陽子、春本晃江
  • Journal Title: Japanese Journal of Protozoology Vol.39(1) Pages: 140-141
• [Journal Article] Sequencing and bioinformatics analysis of ciliate eRF1s toward understanding stop codon recognition (2006)
  • Author(s): キム・ワン、春本晃江
  • Journal Title: Japanese Journal of Protozoology 39(1) Pages: 143-144
• [Journal Article] Ca2+-dependent in vitro contractility of a precipitate isolated from an extract of the heliozoon Actinophrys sol. (2006)
  • Author(s): Arikawa, M., Saito, A., Omura, G., Khan, S.M.M.K., Suetomo, Y., Kakuta, S. and Suzaki, T.
  • Journal Title: Cell Motil. Cytoskel. 63(2) Pages: 57-65
  • Peer Reviewed
• [Journal Article] Axopodial degradation in the heliozoon Raphidiophrys contractilis : A novel bioassay system for detecting heavy metal toxicity in the aquatic environment (2006)
  • Author(s): Khan, S.M. Mostafa Kamal, Yoshimura, C., Arikawa, M., Omura, G., Nishiyama, S., Suetomo, Y., Kakuta, S. and Suzaki, T.
  • Journal Title: Environ. Sci. 13(4) Pages: 193-200
  • Peer Reviewed
• [Journal Article] Bacteria-free culture of a colorless euglenoid, Peranema trichophorum, and establishment of a method for flagellar isolation.Jpn. (2006)
  • Author(s): Suetomo, Y., Saito, A., Arikawa, M., Omura, G., Khan, S.M.M.K., Kakuta, S., Yoshimura, C. and Suzaki, T.
  • Journal Title: J. Protozool. 39 Pages: 37-45
  • Peer Reviewed
• [Journal Article] 繊毛虫ユープロテスの細胞周期 (2006)
  • Author(s): 洲崎敏伸, 有川幹彦
  • Journal Title: 原生動物学雑誌 39 Pages: 77-83
• [Journal Article] ノシメコクガPlodia interpunctellaに寄生するグレガリナについて (2006)
  • Author(s): 洲崎敏伸,宇尾真紀子, 野田博明, 竹田真木生
  • Journal Title: 原生動物学雑誌 39 Pages: 130-131
• [Journal Article] ミドリゾウリムシの細胞内に認められる管状繊維構造 (2006)
  • Author(s): 島田晴美, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 39 Pages: 132-133
• [Journal Article] オクロモナス鞭毛のマスティゴネマの単離とタンパク質の精製 (2006)
  • Author(s): 山田周平, 有川幹彦, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 39 Pages: 133-134
• [Journal Article] Axopodial degradation by arsenic ions and pH in the heliozoon Raphidiophrys contractilis (2006)
  • Author(s): Khan, S. M. M. K. and Suzaki, T.
  • Journal Title: Jpn. J. Protozool 39 Pages: 134-135
• [Journal Article] 太陽虫Actinophrys solの捕食行動におけるエクストルソームと糖タンパク質の役割 (2006)
  • Author(s): 角田宗一郎, 洲崎敏伸
  • Journal Title: 原生動物学雑誌 39 Pages: 136-137
• [Journal Article] 太陽虫を用いた水質モニタリングシステムの試験開発 (2006)
  • Author(s): 吉村知里Khan, S.M.M.K.・洲崎敏伸
  • Journal Title: 原生動物学雑誌 39 Pages: 145-146
• [Journal Article] 実験プラスチックごみ調査 (2006)
  • Author(s): 洲崎敏伸
  • Journal Title: 神戸大学環境管理センター報 2 Pages: 12-14
• [Journal Article] Practical synthesis of blepharismone, a mating inducing pheromone of Blepharisma japonicum (2006)
  • Author(s): Takihiro, H. ; Uruma, Y. ; Usuki, Y. Miyake, A. ; Iio, H.
  • Journal Title: Tetrahedron Asymmetry 17 Pages: 2339-2343
  • Peer Reviewed
• [Journal Article] Blepharisma(albino)、Climacostomum、Dileptusの光増感性色素によるPhotodynamic killing (2006)
  • Author(s): 寺島昌代, 春本晃江
  • Journal Title: Jpn. J. Protozool. 39(1) Pages: 121-123
• [Journal Article] 繊毛虫ブレファリズマで接合誘導時に発現する遺伝子の研究 (2006)
  • Author(s): 杉浦真由美, 田中悠里, 久保陽子, 春本晃江
  • Journal Title: Jpn. J. Protozool. 39(1) Pages: 140-141
• [Journal Article] 繊毛虫における翻訳終結因子eRF1の終止コドン認識機構解明へ向けての解析 (2006)
  • Author(s): キム・ワン, 由良敬, 郷信弘, 春本晃江
  • Journal Title: Jpn. J. Protozool. 39(1) Pages: 143-144
• [Journal Article] Book Review for "Paramecium : Genetics and Epigenetics", by GH Beale and JR Preer, Jr."
  • Author(s): Harumoto T
  • Journal Title: The Quarterly Review of Biology (印刷中)
• [Presentation] 自己防御物質クリマコストールの構造活性相関 (2009)
  • Author(s): 瀬田梢・宮本顕範・田原由樹・粳間由幸・吉岡康一・土江松美・臼杵克之助・飯尾英夫
  • Organizer: 日本化学会第89春季年会(2009)
  • Place of Presentation: 千葉
• [Presentation] スピロストミンの合成研究ースピロストミンAの立体選択的合成研究ースピロストミンAの立体選択的 (2009)
  • Author(s): 世良佳彦・冨永宗平・高山欣子・臼杵克之助・木下勇・飯尾英夫
  • Organizer: 日本化学会第89春季年会(2009)
  • Place of Presentation: 千葉
• [Presentation] 繊毛虫Spirostomum teresの自己防御物質スピロストミンの化学的性質 (2009)
  • Author(s): 冨永宗平・世良佳彦・臼杵克之助・飯尾英夫
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 千葉
• [Presentation] Biosynthetic Studies on Blepharismins : Self-defense Pigments of Blepharisma japonicum (2008)
  • Author(s): Koichi Yoshioka, Sohei Tominaga, Yusuke Inagaki, Naofumi Kaneko, Yoshiyuki Uruma, Yoshinosuke Usuki, and Hideo Iio
  • Organizer: 第13回大阪市立大学大学院理学研究科物質分子専攻『トポロジカル超分子』に関する国際シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-12-03
• [Presentation] Absolute Configuration of Spirostomin, Defense Toxin of the Ciliate Spirostomum teres (2008)
  • Author(s): Yoshihiko Sera, Sohei Tominaga, Yoshiko Takayama, Yoshinosuke Usuki, Isamu Kinoshita and Hideo Iio
  • Organizer: 第13回大阪市立大学大学院理学研究科物質分子専攻『トポロジカル超分子』に関する国際シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-12-03
• [Presentation] スピロストミンの立体選択的合成研究 (2008)
  • Author(s): 世良佳彦・臼杵克之助・飯尾英夫
  • Organizer: 第28回有機合成若手セミナー
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-12
• [Presentation] (±)-Spirostomin AおよびBの立体選択的合成 (2008)
  • Author(s): 世良佳彦・臼杵克之助・飯尾英夫
  • Organizer: 第35回有機反応懇談会
  • Place of Presentation: 大阪
  • Year and Date: 2008-08-01
• [Presentation] 原生動物繊毛虫ブレファリズマの交配フェロモンとしてのトリプトファン誘導体、日本トリプトファン研究会 (2008)
  • Author(s): 杉浦真由美、洲崎敏伸、飯尾英夫、岩崎杉浦真由美、洲崎敏伸、飯尾英夫、岩崎
  • Organizer: 第30回学術集会
  • Place of Presentation: 倉敷市
• [Presentation] Study on diversity of stop codon in ciliates and stop-codon recognition by ciliate eRF1s (2008)
  • Author(s): Kim OTP, Lee Y, Hong TD,Sakurai A and Harumoto T
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Presentation] 原生生物繊毛虫ブレファリズマにおける交配フェロモンの機能 (2008)
  • Author(s): 杉浦真由美、田中悠里、岩崎祥子、吉村千代、沢田菜穂子、洲崎敏伸、春本晃江
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Presentation] 繊毛虫ブレファリズマにおける接合誘導物質ガモン1抗体を用いた受容体の探索 (2008)
  • Author(s): 杉浦真由美、春本晃江、洲崎敏伸
  • Organizer: 第41回日本原生動物学会大会
  • Place of Presentation: 筑波市
• [Presentation] 繊毛虫の翻訳終結因子(eRF1)における終止コドン認識に関わる残基の解析 (2008)
  • Author(s): 李英、キム・ワン、春本晃江
  • Organizer: 第41回日本原生動物学会大会
  • Place of Presentation: 筑波市
• [Presentation] 繊毛虫Blepharisma japonicumにおける接合誘導物質ガモン1の糖鎖の役割 (2008)
  • Author(s): 岩崎祥子、杉浦真由美、春本晃江
  • Organizer: 第41回日本原生動物学会大会
  • Place of Presentation: 筑波市
• [Presentation] 繊毛虫の翻訳終結因子(eRF1)における終止コドン認識に関わる残基の解析 (2008)
  • Author(s): 李英、キム・ワン、春本晃江
  • Organizer: 第79回日本動物学会大会
  • Place of Presentation: 福岡市
• [Presentation] (±)-Spirostomin AおよびBの立体選択的合成 (2008)
  • Author(s): 世良佳彦・臼杵克之助・飯尾英夫
  • Organizer: 第43回天然物化学談話会
  • Place of Presentation: 大阪
• [Presentation] 繊毛虫ブレファリズマが有する赤色色素ブレファリズミンの生合成研究 (2008)
  • Author(s): 吉岡康一・冨永宗平・粳間由幸・臼杵克之助・飯尾英夫
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] 繊毛虫Spirostomum teresの自己防御物質スピロストミンの絶対立体配置 (2008)
  • Author(s): 冨永宗平・臼杵克之助・木下勇・飯尾英夫
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] 異毛目繊毛虫スピロストマムの自己防御物質スピロストミンの不斉合成 (2008)
  • Author(s): 高山欣子・世良佳彦・臼杵克之助・飯尾英夫
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] 繊毛虫ブレフアリズマにおける接合誘導物質ガモン1抗体を用いた受容体の探索 (2008)
  • Author(s): 杉浦真由美, 春本晃江, 洲崎敏伸
  • Organizer: 第41回日本原生動物学会大会
  • Place of Presentation: 筑波市
• [Presentation] 原生動物繊毛虫ブレファリズマの交配フェロモンとしてのトリプトファン誘導体 (2008)
  • Author(s): 杉浦真由美, 洲崎敏伸, 飯尾英夫, 岩崎祥子, 春本晃江
  • Organizer: 日本トジプトフアン研究会第30回学術集会
  • Place of Presentation: 倉敷市
• [Presentation] 原生生物繊毛虫ブレフアリズマにおける交配フェロモンの機能 (2008)
  • Author(s): 杉浦真由美, 田中悠里, 岩崎祥子, 吉村千代, 沢田菜穂子, 洲崎敏伸, 春本晃江
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Presentation] 繊毛虫ブレファリズマが有する自己防御物質ブレファリズミンの生合成開始ユニット (2007)
  • Author(s): 吉岡康一・冨永宗平・粳間由幸・臼杵克之助・飯尾英夫
  • Organizer: 化学プラットフォーム@関西07講演会
  • Place of Presentation: 兵庫
  • Year and Date: 2007-09-15
• [Presentation] 異毛類繊毛虫Blepharisma japonicumが有する自己防御物質blepharisminの生合成開始ユニット (2007)
  • Author(s): 吉岡康一・冨永宗平・粳間由幸・臼杵克之助・飯尾英夫
  • Organizer: 日本原生動物学会第40回大会
  • Place of Presentation: 富山
• [Presentation] ブレファリズマのII型細胞で接合誘導時特異的に発現するcdk1, cks遺伝子の分子的特徴 (2007)
  • Author(s): 田中悠里、杉浦真由美、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] Relation between the stop codon reassignment and the specificity of eukaryotic release factor 1 (eRF1) in Blepharisma (2007)
  • Author(s): キム・ワン、池原健二、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] 繊毛虫ブレファリズマにおける接合誘導物質ガモン1の糖鎖の研究-糖鎖は接合誘導活性に必要か? (2007)
  • Author(s): 岩崎祥子、杉浦真由美、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] 強制飢餓によるガモン1の発現誘導-発現開始と飢餓の関連性- (2007)
  • Author(s): 山中美果、杉浦真由美、田中悠里、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] 繊毛虫Blepharisma stoltei とB. americanumにおけるガモン1相同遺伝子の単離と解析 (2007)
  • Author(s): 三浦満美子、杉浦真由美、春本晃江
  • Organizer: 第78回日本動物学会大会
  • Place of Presentation: 弘前市
• [Presentation] 繊毛虫Didinium nasutumのErf1の終止コドン認識解析への試み (2007)
  • Author(s): 櫻井昭、キム・ワン、春本晃江
  • Organizer: 第78回日本動物学会大会
  • Place of Presentation: 弘前市
• [Presentation] Genes transcriptionally activated during induction of conjugating pairs in mating-type II cells of the ciliate Blepharisma japonicum FASEB Summer Research Conferences (2007)
  • Author(s): Tanaka Y, Sugiura M, Harumoto T
  • Organizer: Ciliate Molecular Biology
  • Place of Presentation: ツーソン(米国)
• [Presentation] Stop codon recognition by eRF1s in Litostome ciliates、FASEB Summer Research Conferences、 (2007)
  • Author(s): Kim OTP, Sakurai A and Harumoto T
  • Organizer: Ciliate Molecular Biology
  • Place of Presentation: ツーソン(米国)
• [Presentation] Specifically regulated expression of gamone 1: the trigger molecule for conjugation in Blepharisma japonicum FASEB Summer Research Conferences (2007)
  • Author(s): Sugiura M, Kawahara S, Teramoto K, Kobayashi K, Takami R, Yamanaka M, Tanaka Y and Harumoto T
  • Organizer: Ciliate Molecular Biology
  • Place of Presentation: ツーソン(米国)
• [Presentation] Genes transcriptionally activated during induction of conjugating pairs in mating-type II cells of the ciliate Blepharisma japonicum (2007)
  • Author(s): Tanaka Y, Sugiura M and Harumoto T
  • Organizer: FASEB Summer Research Conference Ciliate Molecular Biology
  • Place of Presentation: Tucson, Arizona, US
• [Presentation] Stop codon recognition by eRF1s in Litostome ciliates (2007)
  • Author(s): Kim OTP, Sakurai A and Harumoto T
  • Organizer: FASEB Summer Research Conference Ciliate Molecular Biology
  • Place of Presentation: Tucson, Arizona, US
• [Presentation] Specifically regulated expression of gamone 1: the trigger molecule for conjugation in Blepharisma japonicum (2007)
  • Author(s): Sugiura M, Kawahara S, Teramoto K, Kobayashi K, Takami R, Yamanaka M, Tanaka Y and Hanumoto T
  • Organizer: FASEB Summer Research Conference Ciliate Molecular Biology
  • Place of Presentation: Tucson, Arizona, US
• [Presentation] Gene expression toward conjugation in Blepharisma japonicum (2007)
  • Author(s): Harumoto, T
  • Organizer: FASEB Summer Research Conference Ciliate Molecular Biology
  • Place of Presentation: Tucson, Arizona, US
• [Presentation] 繊毛虫Blepharisma stolteiとB. americanum におけるガモン1相同遺伝子の単離と解析 (2007)
  • Author(s): 三浦満美子、杉浦真由美、春本晃江
  • Organizer: 第78回日本動物学会大会
  • Place of Presentation: 弘前市
• [Presentation] 繊毛虫Didinium nasutumのeRF1の終止コドン認識解析への試み (2007)
  • Author(s): 櫻井昭、キム・ワン、春本晃江
  • Organizer: 第78回日本動物学会大会
  • Place of Presentation: 弘前市
• [Presentation] ブレファリズマのII型細胞で接合誘導時特異的に発現するcdkl, cks遺伝子の分子的特徴 (2007)
  • Author(s): 田中悠里、杉浦真由美、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] Relation between the stop codon reassignment and the specificity of eukaryotic release factor 1(eRF1)in Blepharisma (2007)
  • Author(s): キム・ワン、池原健二、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] 繊毛虫ブレファリズマにおける接合誘導物質ガモン1の糖鎖の研究-糖鎖は接合誘導活性に必要か?- (2007)
  • Author(s): 岩崎祥子、杉浦真由美、春本晃江
  • Organizer: 第40回日本原生動物学会大会
  • Place of Presentation: 富山市
• [Presentation] 繊毛虫における接合前細胞間相互作用 (2006)
  • Author(s): 春本晃江、杉浦真由美
  • Organizer: シンポジウム「原生動物の細胞認識と相互作用」日本動物学会第77回大会
  • Place of Presentation: 松江市(招待講演)
  • Year and Date: 2006-09-21
• [Presentation] How is ciliate eukaryotic release factor 1 (eRF1) different from conventional eRF1s? (2006)
  • Author(s): キム・ワン、池原健二、春本晃江
  • Organizer: 第39回日本原生動物学会大会
  • Place of Presentation: 佐賀市
• [Presentation] 繊毛虫Blepharisma japonicumにおけるガモン1相同遺伝子の検出と配列決定 (2006)
  • Author(s): 三浦満美子、杉浦真由美、春本晃江
  • Organizer: 第39回日本原生動物学会大会
  • Place of Presentation: 佐賀市
• [Presentation] Didinium nasutumのeRF1の終止コドン認識解析への試み (2006)
  • Author(s): 櫻井昭、キム・ワン、春本晃江
  • Organizer: 第39回日本原生動物学会大会
  • Place of Presentation: 佐賀市
• [Presentation] 繊毛虫ブレファリズマ接合型II型細胞の接合誘導時に特異的に発現する遺伝子 (2006)
  • Author(s): 田中悠里、杉浦真由美、春本晃江
  • Organizer: 日本動物学会第77回大会
  • Place of Presentation: 松江市
• [Book] IFO微生物学(大嶋泰治監修)第14章 (2008)
  • Author(s): 洲崎敏伸
  • Publisher: 原生動物培風館(印刷中)
• [Book] 身近な動物を使った実験書 (2008)
  • Author(s): 春本晃江、洲崎敏伸、ほか
  • Publisher: 三共出版(印刷中)
• [Book] 「ゾウリムシなどの繊毛虫を用いた実験法」鈴木範男編「身近な動物を使った実験」
  • Author(s): 春本晃江、洲崎敏伸
  • Publisher: 三共出版(印刷中)
• [Book] 「ゾウリムシなどの繊毛虫を用いた実験法」鈴木範男編「身近な動物を使った実験」
  • Author(s): 春本晃江, 洲崎敏伸
  • Publisher: 三共出版(印刷中)