Study on the expression and function of sex pheromone in ciliates
| Project/Area Number |
18570129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nara Women's University |
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Principal Investigator |
HARUMOTO Terue Nara Women's University, 理学部, 教授 (80198936)
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| Co-Investigator(Kenkyū-buntansha) |
SUZAKI Toshinobu 神戸大学, 大学院・理学研究科, 准教授 (00187692)
IIO Hideo 大阪市立大学, 大学院・理学研究科, 教授 (80145771)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,070,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | ホルモンと生理活性物質 / 繊毛虫 / 接合 / タンパク質 / 発現調節 / 糖鎖 / 受容体 / 糖タンパク質 / 大量発現系 / rRNA遺伝子 / 種間関係 / cdk / cks / 4-HPPD / 原生動物 / 相同遺伝子 / 接合型 / 性フェロモン / ブレファリズマ / 有性生殖 / 遺伝子 / PCR |
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| Research Abstract |
原生生物繊毛虫Blepharismaの接合誘導時特異的に発現する遺伝子を単離・同定した。糖鎖を欠く接合誘導物質ガモン1を(1) 酵素処理、および(2) 大腸菌を用いた大量発現系により得て、ガモン1の糖鎖は誘導活性に重要な役割をもつことを明らかにした。ガモン1に対する抗体を作製し、II型細胞表面にあるガモン1受容体の探索を行った。野外で採集したBlepharismaの種を同定し、rRNA遺伝子とガモン1遺伝子の配列を調べ、Blepharismaの種の分岐について考察した。
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Report
(4 results)
Research Products
(119 results)
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[Journal Article] Ciliates use both variant and universal genetic codes : evidence of omnipotent eRF1s in the class Litostomatea2008
Author(s)
Kim, OTP, Sakurai, A, Saito, K, Ito, K, Ikehara, K and Harumoto, T
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Journal Title
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Peer Reviewed
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[Journal Article] Implications of microbial symbionts on defense and competition of hosts-ciliates2008
Author(s)
Gortz, H. -D., Rosati, G., Schweikert, M., Schrallhammer, M., Omura, G. and Suzaki, T.
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Journal Title
"Defensive Mutalism in Microbial Symbiosis" (ed. : Jim White and Monica Torres) (in press)
Related Report
Peer Reviewed
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[Journal Article] Ciliates use both variant and universal genetic codes : evidence of omnipotent eRF1s in the class Litostomatea2008
Author(s)
Kim, OTP, Sakurai, A, Saito, K, Ito, K, Ikehara, K, Harumoto, T
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Ciliates use both variant and universal genetic codes: evidence of omnipotent eRF1s in the class Litostomatea2008
Author(s)
Kim, O. T. P., Sakurai, A., Saito, K., Ito, K., Ikehara, K. and Harumoto, T.
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Journal Title
Gene DOI link: http://dx.doi.org/10.1016/j.gene.2008.03.018 (In press)
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[Journal Article] Ca2+-dependent in vitro contractility of a precipitate isolated from an extract of the heliozoon Actinophrys sol.2006
Author(s)
Arikawa, M., Saito, A., Omura, G., Khan, S.M.M.K., Suetomo, Y., Kakuta, S. and Suzaki, T.
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Journal Title
Cell Motil. Cytoskel. 63(2)
Pages: 57-65
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Peer Reviewed
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[Journal Article] Axopodial degradation in the heliozoon Raphidiophrys contractilis : A novel bioassay system for detecting heavy metal toxicity in the aquatic environment2006
Author(s)
Khan, S.M. Mostafa Kamal, Yoshimura, C., Arikawa, M., Omura, G., Nishiyama, S., Suetomo, Y., Kakuta, S. and Suzaki, T.
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Journal Title
Environ. Sci. 13(4)
Pages: 193-200
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Peer Reviewed
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[Journal Article] Bacteria-free culture of a colorless euglenoid, Peranema trichophorum, and establishment of a method for flagellar isolation.Jpn.2006
Author(s)
Suetomo, Y., Saito, A., Arikawa, M., Omura, G., Khan, S.M.M.K., Kakuta, S., Yoshimura, C. and Suzaki, T.
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Journal Title
J. Protozool. 39
Pages: 37-45
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