Elucidation of novel cAMP signal transductions in myxobacteria

• Project/Area Number: 18570131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kagawa University
• Principal Investigator: KIMURA Yoshio Kagawa University, 農学部, 教授 (10243750)
• Co-Investigator(Kenkyū-buntansha): 竹川 薫 香川大学, 農学部, 教授 (50197282)
• Co-Investigator(Renkei-kenkyūsha): TAKEGAWA Kaoru 九州大学, 大学院・農学研究科, 教授 (50197282)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: PKA / セリン / スレオニンプロテインキナーゼ / cAMP / シグナル伝達 / 細菌

Research Abstract

粘液細菌よりcAMP依存性プロテインキナーゼ(PKA)の検索を試みた結果、PKAの特異的基質を最もよい基質とし, PKAの特異的阻害剤によって活性阻害を受けるSpkAキナーゼを得たが、真核生物のPKAで見られるcAMPと調節サブユニットによる酵素活性調節は見られなかった。
また、本菌のcAMP分解酵素は、その変異株より、胞子の発芽時の浸透圧ストレス応答に関与していると推定し、また、酵素学的諸性質の検討から、基質特異性の広い新規性の高い酵素であった。

Research Products

• [Journal Article] Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus (2009)
  • Author(s): Kimura, Y., Kakemizu, A. Matsubara, Y., and Takegawa, K
  • Journal Title: Journal of Biosci-ence and Bioengineering 107 Pages: 10-15
  • NAID: 110007042019
  • Peer Reviewed
• [Journal Article] Myxococcus xanthus CbpC and CbpD containing two cAMP-binding domains are involved in tem-perature tolerance (2009)
  • Author(s): Ishibashi, K., and Kimura, Y.
  • Journal Title: Technical bulletin of facul-ty of agriculture, Kagawa University 61
  • NAID: 120007012856
• [Journal Article] Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3', 5'- and 2', 3'-cAMP phosphodiesterase, and phosphatase activities. (2009)
  • Author(s): Kimura, Y., Okazaki, N., Takegawa, K.
  • Journal Title: FEBS Letters 583 Pages: 443-448
  • Peer Reviewed
• [Journal Article] Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus. (2009)
  • Author(s): Kimura, Y., Kakemizu, A., Matsubara, Y., Takegawa, K.
  • Journal Title: Journal of Bioscience and Bioengineering 107 Pages: 10-15
  • NAID: 110007042019
  • Peer Reviewed
• [Journal Article] Myxococcus xanthus CbpC and CbpD containing two cAMP-bindingdomains are involved in temperature tolerance. (2009)
  • Author(s): Ishibashi, K., Kimura, Y.
  • Journal Title: Technical bulletin of faculty of agriculture, Kagawa University 61 Pages: 47-53
• [Journal Article] Enzymatic characteristics of two novel Myxococcus xanthus enzymes (2008)
  • Author(s): Kimura, Y., Okazaki, N., and Takegawa, K
  • Journal Title: PdeA and PdeB, displaying 3', 5'- and 2', 3'-cAMP phospho- diesterase, and phosphatase activities FEBS Letters 583 Pages: 443-448
  • Peer Reviewed
• [Journal Article] Anal-ysis of functional properties of PirA and ScdA in Myxococcus xanthus (2007)
  • Author(s): Kimura, Y., and Hamanaka, H.
  • Journal Title: Technical bulletin of faculty of agriculture, Kagawa University 59 Pages: 79-84
  • NAID: 110006270672
• [Journal Article] Analysis of functional properties of PirA and ScdA in Myxococcus xanthuus (2007)
  • Author(s): Yoshio Kimura
  • Journal Title: Technical bulletin of faculty of agriculture, Kagawa University 59 Pages: 79-84
• [Journal Article] Contribution of the cyclic nucleotide phosphodiesterases PdeA and PdeB to adaptation of Myxococcus xanthus cells to osmotic or high-temperature stress (2006)
  • Author(s): Kimura, Y., Nakatuma, H, Sato, N., and Ohtani, M.
  • Journal Title: Journal of Bacteriology 188 Pages: 823-828
  • Peer Reviewed
• [Journal Article] Contribution of cyclic nucleotide phosphodiesterases, PdeA and PdeB, in adaptation of Myxococcus xanthus cells to osmotic or high temperature stress. (2006)
  • Author(s): Y.Kimura
  • Journal Title: Journal of Bacteriology 188 Pages: 823-828
• [Presentation] 粘液細菌におけるレセプター型アデニル酸シクラーゼの機能解析 (2009)
  • Author(s): 木村義雄
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 粘液細菌Myxococcus xanthusのヒスチジンキナーゼMokAは浸透圧耐性と分化に必要である (2008)
  • Author(s): 木村義雄
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Myxococcus xanthusセリン/スレオニンプロテインキナーゼSpkAの酵素学的特徴 (2008)
  • Author(s): 田上駿一、木村義雄
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Myxococcus xanthus twin-arginine transloca-tion system is important for growth and development (2007)
  • Author(s): 木村義雄、財賀大行、浜中裕子、的場秀樹
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Myxococcus xanthus twin-arginine translocation system is important for growth and development. (2007)
  • Author(s): 木村 義雄
  • Organizer: BMB2007
  • Place of Presentation: 横浜、国際展示場
  • Year and Date: 2007-12-14
• [Presentation] Myxococcus xanthus CbpB, CbpC, and CbpD containing two cAMP-binding domains is involved in temperature and osmotic tolerances (2006)
  • Author(s): Ishibashi, K., Kobayashi, S., Nakato, H., and Kimura, Y.
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 横浜
  • Year and Date: 2006-12-07
• [Presentation] Contribution of cyclic nucleotide phosphodiesterases, PdeA and PdeB, in adaptation of Myxococcus xanthus cells to osmotic or high temperature stress (2006)
  • Author(s): Kimura, Y., Nakatuma, H., Sato, N., and Ohtani, M.
  • Organizer: stress. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (2006)
  • Place of Presentation: 京都
  • Year and Date: 2006-06-21