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Discovery and analysis of dovel lipid signal transduction complexes

Research Project

Project/Area Number 18570132
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionSapporo Medical University

Principal Investigator

SAKANE Fumio  Sapporo Medical University, Department of Biochemistry, School of Medicine, Associate Professor (10183815)

Co-Investigator(Kenkyū-buntansha) KANOH Hideo  Sapporo Medical University, Department of Biochemistry, School of Medicine, Honorary Professor (70045475)
IMAI Shin-ichi  Sapporo Medical University, Department of Biochemistry, School of Medicine, Assistant Professor (20213209)
KAI Masahiro  Sapporo Medical University, Department of Biochemistry, School of Medicine, Assistant Professor (80260777)
安田 智  札幌医科大学, 医学部, 助教 (20381262)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsDiacylglycerol kinase / Epidermal growth factor receptor / Protein kinase C / Apoptosis / NF-κB / Rac1 / β2-Chimaerin / Insulin receptor / Racl / ホスファチジン酸ホスファターゼ
Research Abstract

Diacylglycerol (DAG) kinase (DGK) modulates the balance between the two signaling lipids, DAG and phosphatidic acid (PA), by phosphorylating DAG to yield PA. To date, ten mammalian DGK isozymes have been identified. Beyond our expectations, recent studies have revealed that DGK isozymes play pivotal roles in a wide variety of signal transduction pathways.
Recently, we found that DGKa is expressed in several human melanoma cell lines but not in noncancerous melanocytes. Intriguingly, the overexpression of wild-type DGKa, but not of its kinase-dead mutant, markedly suppressed tumor necrosis factor-a-induced apoptosis of AKI human melanoma cells. In the reverse experiment, small interfering RNA-mediated knockdown of DGKα significantly enhanced the apoptosis, thus convincingly indicating that this isoform possesses a cytoprotective function. Moreover, we obtained several lines of evidence suggesting that DGKa suppresses tumor necrosis factor-a-induced melanoma cell apoptosis through activat … More ion of nuclear factor κB. Thus, this transcription factor is likely to be one of the key factors downstream of DGKa during cell proliferation and survival.
We had already demonstrated that DGKγ functions through its catalytic action as an upstream suppressor of Racl, and consequently, lamellipodium/membrane ruffle formation. Recently, we further found that DGKγ specifically interacts with and activates β2-chimaerin, a Rac-specific GTPase-activating protein, in response to cell stimulation with epidermal growth factor (EGF) and hydrogen peroxide/phorbol ester. These results suggest that β2-chimaerin is an effector molecule linking DGKγ functionally with Racl.
We generated and then analyzed DGKO-knockout mice. DGKδ deficiency reduced EGF receptor protein expression and activity. DAG accumulation caused by DGK8 deficiency activated PKC, which phosphorylates EGF receptor at Thr-654. The phosphorylation consequently inactivates the receptor and enhances its degradation. In this case, DGKδ was associated with conventional protein kinase C (PKC) (PKCα) and novel PKC (PKC_s, δ, a and η). Thus, DGKδ is concluded to regulate EGF receptor, at least in part, by modulating PKC signaling.
We further reported that antisense silencing of DGKδ was sufficient to prevent the effect of high glucose on PKCα activity, insulin receptor signaling and glucose uptake. Thus, the short-term exposure of skeletal muscle cells to glucose caused a rapid induction of DGKδ, followed by a reduction of PKCα activity and transactivation of the insulin receptor signaling. Moreover, we identified decreased DGKδ expression, concomitant with reduced DGK activity in skeletal muscle from type II diabetic patients. In diabetic animals, reduced DGK8 protein and kinase activity were restored upon correction of glycemia. DGKδ haploinsufficiency increased DAG content and caused peripheral insulin resistance, impaired skeletal muscle insulin signaling and glucose transport, and age-dependent obesity. The impairment was associated with reduced insulin-stimulated turosine phosphorylation of insulin receptor substrate-1 and serine/threonine phosphorylation of Akt and the RabGAP AS160, components of the canonical insulin signaling cascade important for glucose uptake and metabolism in skeletal muscle. Reduced DGKδ protein expression directly contributes to the development of peripheral insulin resistance and mild obesity. Therapeutic approaches to target DAG and PA metabolism via regulation of DGKδ may control and prevent insulin resistance in metabolic disease. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (70 results)

All 2008 2007 2006 Other

All Journal Article (38 results) (of which Peer Reviewed: 19 results) Presentation (27 results) Remarks (2 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Rab7 regulates maturation of the melanosomal matrix protein gp100/Pmel17/Silv2008

    • Author(s)
      Kawakami, A., et. al.
    • Journal Title

      J.Invest.Dermatol. 128

      Pages: 143-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase γ with the Src homology 2 and Cl domains of β2-chimaedn2008

    • Author(s)
      Yasuda, S., et. al.
    • Journal Title

      Biochem.J. 409

      Pages: 95-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Down-regulation of diacylglycerol kinase delta contributes to hyperglycemia-induced insulin resistance2008

    • Author(s)
      Chibalin, A. V., et. al.
    • Journal Title

      Cell 132

      Pages: 375-386

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Regulation of enzyme localization by polymerization: Polymer formation by the SAM domain of diacylglycerol kinase δ12008

    • Author(s)
      Harada, B. T., et. al.
    • Journal Title

      Structure 16

      Pages: 380-387

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Rab7 regulates maturation of the melanosomal matrix protein gp100/Pmel17/Sil2008

    • Author(s)
      Kawakami, A., Sakane, F., Imai, S., Yasuda, S., Kai, M., Kanoh, H., Jin, H-Y., Hirosaki, K., Yamashita, T., Fisher, D. E., Jimbow, K
    • Journal Title

      J. Invest. Dermatol 128 (1)

      Pages: 143-150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase y with the Src homology 2 and Cl domains of β2-chimaerin2008

    • Author(s)
      Yasuda, S., Kai, M., Imai, S., Kanoh, H., Sakane, F
    • Journal Title

      Biochem. J 409 (1)

      Pages: 95-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Down-regulation of diacylglycerol kinase delta contributes to hyperglycemia-induced insulin resistance2008

    • Author(s)
      Chibalin, A., V., Leng, Y., Vieira, E., Krook, A., Bjornholm, M., Long, Y-C., Kotova, O., Zhong, Z., Sakane, F., Steiler, T., Nylen, C., Wang, J., Laakso, M., Topham, M. K., Gilbert, M., Wallberg-Henriksson, H., Zierath, J. R
    • Journal Title

      Cell 132(3)

      Pages: 375-386

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Regulation of enzyme localization by polymerization : Polymer formation by the SAM domain of diacylglycerol kinase 812008

    • Author(s)
      Harada, B., T., Knight, M., J., Imai, S., Qiao, F., Ramachander, R., Sawaya, M. R., Gingery, M., Sakane, F.,d Bowie, J. U
    • Journal Title

      Structure 16(3)

      Pages: 380-387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Rab7 regulates maturation of the melanosomal matrix protein gp100/Pme117/Silv2008

    • Author(s)
      Kawakami, A., et. al.
    • Journal Title

      J. Invest. Dermatol. 128

      Pages: 143-150

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phorbol ester and hydrogen peroxide synergistically induce the interaction of diac ylglycerol kinase γ with the Src homology 2 and C1 domains of β2-chimaerin2008

    • Author(s)
      Yasuda, S., et. al.
    • Journal Title

      Biochem. J. 409

      Pages: 95-106

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinase γ interacts with and activates β2-chimaerin,a Rac-specific GAP,in response to epidermal growth factor2007

    • Author(s)
      Yasuda, S., et. al.
    • Journal Title

      FEBS Lett. 581

      Pages: 551-557

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinase α suppresses tumor necrosis factor-α-induced apoptosis of human melanoma cells through NF-κB activation2007

    • Author(s)
      Yanagisawa, K., et. al.
    • Journal Title

      Biochim.Biophys.Acta-Mol.Cell Biol.Lipids 1771

      Pages: 462-474

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinases: Why so many of them?2007

    • Author(s)
      Sakane, F., et. al.
    • Journal Title

      Biochim.Biophys.Acta-Mol.Cell Biol.Lipids 1771

      Pages: 793-806

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tyrosine phosphorylation of β2-chimaerin by Src-family kinase negatively regulate s its Rac-specific GAP activity2007

    • Author(s)
      Kai, M., et. al.
    • Journal Title

      Biochim.Biophys.Acta-Mol.Cell Res. 1773

      Pages: 1407-1415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Glucose regulates diacylglycerol intracellular levels and protoin kinase C activity by modulating diacylglycerol-kinase subcellular localization2007

    • Author(s)
      Miele, C., et. al.
    • Journal Title

      J.Biol.Chem. 282

      Pages: 31835-31843

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 「研究成果報告書概要(欧文)」より2007

    • Author(s)
      Yanagisawa, K., Yasuda, S., Kai, M., Imai, S., Yamada, K., Yamashita, T., Jimbow, K., Kanoh, H., Sakane, F
    • Journal Title

      Biochim. Biophys. Acta - Mol. Cell Biol. Lipids 1771 (4)

      Pages: 462-474

    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Diacylglycerol kinase γ interacts with and activates β2-chimaerin, a Rae-specific GAP, in response to epidermal growth factor2007

    • Author(s)
      Yasuda, S., Kai, M., Imai, S., Kanoh, H., Sakane, F
    • Journal Title

      FEBS Lett 581(3)

      Pages: 551-557

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Diacylglycerol kinases : Why so many of them?2007

    • Author(s)
      Sakane, F., Imai, S., Kai, M., Yasuda, S., Kanoh, H
    • Journal Title

      Biochim. Biophys. Acta - Mol. Cell Biol. Lipids 1771(7)

      Pages: 793-806

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] yrosine phbsphorylation of β2-chimaerin by Src-family kinase negatively regulates its Rae-specific GAP activity2007

    • Author(s)
      Kai, M., Yasuda, S., Imai, S., Kanoh, H., Sakane, F
    • Journal Title

      Biochim. Biophys. Acta - Mol. Cell Res 1773 (9)

      Pages: 1407-1415

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Glucose regulates diacylglycerol intracellular levels and protein kinase C activity by modulating diacylglycerol-kinase subcellular localization2007

    • Author(s)
      Miele, C., Paturzo, F., Teperino, R., Sakane, F., Fiory, F., Oriente, F., Ungaro, P., Valentino, R., Beguinot, F. and Formisano, P
    • Journal Title

      J. Biol. Chem 282 (44)

      Pages: 31835-31843

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Diacylglycerol kinase α suppresses tumor necrosis factor-α-induced apoptosis of human melanoma cells through NF-κB activation2007

    • Author(s)
      Yanagisawa, K., et. al.
    • Journal Title

      Biochim. Biophys. Acta-Mol. Cell Biol. Lipids 1771

      Pages: 462-474

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tyrosine phosphorylation of β2-chimaerin by Src-family kinase negatively regulates its Rac-specific GAP activity2007

    • Author(s)
      Kai, M., et. al.
    • Journal Title

      Biochim. Biophys. Acta-Mol. Cell Res. 1773

      Pages: 1407-1415

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinases: Why so many of them?2007

    • Author(s)
      Sakane, F., et. al.
    • Journal Title

      Biochim. Biophys. Acta-Mol. Cell Biol. Lipids 1771

      Pages: 793-806

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glucose regulates diacylglycerol intracellular levels and protein kinase C activity by modulating diacylglycerol-kinase subcellular localization2007

    • Author(s)
      Miele, C., et. al.
    • Journal Title

      J. Biol. Chem. 282

      Pages: 31835-31843

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinase γ interacts with and activates β2-chimaerin, a Rac-specific GAP, in response to epidermal growth factor2007

    • Author(s)
      yasuda, S, et al.
    • Journal Title

      FEBS Lett. 581(3)

      Pages: 551-557

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diacylglycerol kinase δ regulates protein kinase C and epidermal growth factor receptor signaling2006

    • Author(s)
      Crotty, T., et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 103

      Pages: 15485-15490

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lipid phosphate phosphatases 1 and 3 are localized in distinct lipid rafts2006

    • Author(s)
      Kai, M., et. al.
    • Journal Title

      J.Biochem. 140

      Pages: 677-686

    • NAID

      10018841002

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinase δ regulates protein kinase C and epidermal growth factor receptor signaling2006

    • Author(s)
      Crotty, T., Cai, J., Sakane, F., Taketomi, A., Prescott, S., M., Topham, M., K
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A 103(42)

      Pages: 15485-15490

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lipid phosphate phosphatases 1 and 3 are localized in different lipid rafts2006

    • Author(s)
      Kai, M., Sakane, F., Jia, Y-J., Imai, S., Yasuda, S., Kanoh, H
    • Journal Title

      J. Biochem 140 (5)

      Pages: 677-686

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nuclear transportation of diacylglycerol kinase γ and its possible function in the nucleus2006

    • Author(s)
      Matsubara, T, et al.
    • Journal Title

      J.Biol.Chem. 281(10)

      Pages: 6152-6164

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diacylglycerol kinase & regulates protein kinase C and epidermal growth factor receptor signaling2006

    • Author(s)
      Crotty, T, et al.
    • Journal Title

      Proc.Natl.Acad.Sci. USA 103(42)

      Pages: 15485-15490

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lioid phosphate phosphatases 1 and 3 are localized in distinct lipid rafts2006

    • Author(s)
      Kai, M, et al.
    • Journal Title

      J.Biochem. 140(5)

      Pages: 677-686

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diacylglycerol kinases as emerging potential drug tazgets for a variety of diseases

    • Author(s)
      Sakane, F., et. al.
    • Journal Title

      Curr.Drug Targets (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinases as emerging potential drug targets for a variety of diseases. Special issue "Peripheral proteins as drug targets"

    • Author(s)
      Sakane, F., Imai, S., Kai, M., Yasuda, S., Kanoh, H
    • Journal Title

      Curr. Drug Targets (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Diacylglycerol kinases as emerging potential drug targets for a variety of diseases

    • Author(s)
      Sakane, F., et. al.
    • Journal Title

      Curr. Drug Targets (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglycerol kinase α suppresses tumor necrosis factor-α-induced apoptosis of human melanoma cells through cells through NF-kB activation

    • Author(s)
      Yanagisawa, K, et al.
    • Journal Title

      Biochim.Biophys.Acta-Mol.Cell Biol.Lipids (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tyrosine phosphorylation of β2-chimaerin by Src-family kinase negatively regulates its Rac-specific GAP activity

    • Author(s)
      Kai, M, et al.
    • Journal Title

      Biochim.Biophys.Acta-Mol.Cell Res. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diacylglycerol kinases : Why so many of them?

    • Author(s)
      Sakane, F, et al.
    • Journal Title

      Biochim.Biophys.Acta-Mol.Cell Biol.Lipids (in press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼが制御する多彩な生理機能(シンポジウム「グリセリドとそのリン酸化体の産生・相互変換とそれらが織りなす細胞内外のシグナルネットワーク」)2007

    • Author(s)
      坂根 郁夫
    • Organizer
      第49回日本脂質生化学会研究集会
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] ジアシルグリセロールキナーゼγは細胞刺激に応答しβ2-chimaerin(Rac特異的GAP)を活性化する2007

    • Author(s)
      安田 智, ら
    • Organizer
      第49回日本脂質生化学会研究集会
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] β2-chimaerinのチロシンリン酸化によるRac特異的GAP活性の抑制2007

    • Author(s)
      甲斐 正広, ら
    • Organizer
      第49回日本脂質生化学会研究集会
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 細胞刺激に応答したジアシルグリセロールキナーゼYによるβ2-chimaerin活性化機構2007

    • Author(s)
      安田 智, ら
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] EGF刺激に応答したβ2-chimaerinのチロシンリン酸化とそのGAP活性の抑制2007

    • Author(s)
      甲斐 正広, ら
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] ジアシルグリセロールキナーゼδとRACK1は互いの複数のドメインを介して結合している2007

    • Author(s)
      今井 伸一, ら
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 細胞刺激に応答したジアシルグリセロールキナーゼγによるβ2-chimaerin活性化機構2007

    • Author(s)
      安田智, ら
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼδとRACKIは互いの複数のドメインを介して結合している2007

    • Author(s)
      今井伸一, ら
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼが制御する多彩な生理病理機能-発生,脳神経系,免疫系,細胞骨格系,アポトーシス,発がん,糖尿病における役割-2006

    • Author(s)
      坂根 郁未, ら
    • Organizer
      第43回日本生化学会北海道支部例会
    • Place of Presentation
      札幌
    • Year and Date
      2006-11-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rab7 interacts with the melanosomal matrix protein gp100/Pme117/Silv and regulates its maturation in MMAc human melanoma cells2006

    • Author(s)
      Kawakami, A., et. al.
    • Organizer
      67th Annual Meeting of the Society for Investigative Dermetology
    • Place of Presentation
      Philladelphia,Pennsylvania,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase α suppresses TNF-α-induced apoptosis of human melanoma cells through activation of NF-κB2006

    • Author(s)
      Yanagisawa, K., et. al.
    • Organizer
      67th Annual Meeting of the Society for Investigative Dermetology
    • Place of Presentation
      Philladelphia,Pennsylvania,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] AKIメラノーマ細胞においてジアシルグリセロールキナーゼαはNF-κB活性化を介してTNF-α-誘導アポトーシスを抑制する2006

    • Author(s)
      柳澤 健二, ら
    • Organizer
      日本研究皮膚科学会 第31回年次学術大会・総会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] MMAcヒトメラソーマ細胞においてRab7はメラノソームマトリクス蛋白質gp1OO/Pmell7/Silと相互作用し,その成熟に関与する2006

    • Author(s)
      川上 聡経, ら
    • Organizer
      日本研究皮膚科学会 第31回年次学術大会・総会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase δ binds to RACK1,receptor for activated C kinase 12006

    • Author(s)
      Imai, S., et. al.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase α suppresses TNF-α-induced apoptosis of human melanoma cells through activation of NF-κB2006

    • Author(s)
      Yanagisawa, K., et. al.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Src-family kinase and phorbol ester/diacylglycerol signals cooperate to phosphorylate and activate β2-chimaerin,a Rac-specific GAP2006

    • Author(s)
      Kai, M., et. al.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of β2-chimaerin by diacylglycerol kinase γ2006

    • Author(s)
      Yasuda, S., et. al.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rab7 interacts with the melanosomal matrix protein gp100/Pme117/Siltv and regulates its maturation in MMAc human melanoma cells2006

    • Author(s)
      Kawakami, A., et. al.
    • Organizer
      13th Meeting of the European Society for Pigment Cell Research
    • Place of Presentation
      Barcelona,Spain
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase α suppresses TNF-α-induced apoptosis of human melanoma cells through activation of NF-κB2006

    • Author(s)
      Yanagisawa, K., et. al.
    • Organizer
      13th Meeting of the European Society for Pigment Cell Research
    • Place of Presentation
      Barcelona,Spain
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rab7 interacts with the melanosomal matrix protein gp100/Pme117/Silv and regulates its maturation in MMAc human melanoma cells2006

    • Author(s)
      Kawakami, A., Sakane, F., Imai, S., Kanoh, H., Oukura, M., Yamashita, T., Jimbow, K
    • Organizer
      7th Annual Meeting of the Society for InvestigativeK.Dermetology. Philladelphia, Pennsylvania
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase a suppresses TNF-a-induced apoptosis of human melanoma cells through activation of NF-KB2006

    • Author(s)
      Yanagisawa, K., Jimbow, K., Kanoh, H., Sakane, F
    • Organizer
      7th Annual Meeting of the Society for InvestigativeK.Dermetology. Philladelphia, Pennsylvania
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase 8 binds to RACK1, receptor for activated C kinase 12006

    • Author(s)
      Imai, S., Yasuda, S., Kai, M., Sakane, F., Kanoh, H
    • Organizer
      20th IUBMB International Congress of Biochemist ry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase α suppresses TNF-a-induced apoptosis of human melanoma cells through activation of NF-K132006

    • Author(s)
      Yanagisawa, K., Jimbow, K., Yamada, K., Kanoh, H., Sakane, F
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Src-family kinase and phorbol ester/diacylglycerol signals cooperate to phosphorylate and activate β2-chimaerin, a Rac-specific GAP2006

    • Author(s)
      Kai, M., Yasuda, S., Imai, S., Sakane, F., Kanoh, H
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and Ilth FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of β2-chimaerin by diacylglycerol kinasey2006

    • Author(s)
      Yasuda, S., Kai, M., Imai, S., Sakane, F., Kanoh, H
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rab7 interacts with the melanosomal matrix protein gp100/Pme117/Silv and regulates its maturation in MMAc human melanoma cells2006

    • Author(s)
      Kawakami, A., Sakane, F., Imai, S., Kanoh, H., Oukura, M., Hirosaki, K., Yamashita, T., Jimbow, K
    • Organizer
      13th Meeting of the European Society for Pigment Cell Research. Barcelona
    • Place of Presentation
      Spain
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diacylglycerol kinase a suppresses TNF-a-induced apoptosis of human melanoma cells through activation of NF-κB2006

    • Author(s)
      Yanagisawa, K., Jimbow, K., Kanoh, H., Sakane, F
    • Organizer
      13th Meeting of the European Society for Pigment Cell Research. Barcelona
    • Place of Presentation
      Spain
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://web.sapmed.ac.jp/biochem2/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://web.sapmed.ac.jp/biochem2/

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] DGKα阻害剤を含有する抗がん剤2007

    • Inventor(s)
      坂根 郁夫ら
    • Industrial Property Rights Holder
      北海道
    • Filing Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] Method of screening for agents that regulate the shedding of membrane bound proteins and methods of use2007

    • Inventor(s)
      Prescott, S. M., et. al.
    • Industrial Property Rights Holder
      University of Utah Research Foundation, Salt Lake City (USA)
    • Filing Date
      2007-07-10
    • Related Report
      2007 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] DGK_α阻害剤を含有する抗がん剤2007

    • Inventor(s)
      坂根郁夫ら
    • Industrial Property Rights Holder
      北海道
    • Filing Date
      2007-03-29
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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