| Project/Area Number |
18570140
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
|
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| Research Institution | Doshisha Women's College of Liberal Arts |
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Principal Investigator |
WADA Ge-Hong Sun Doshisha Women's College of Liberal Arts, Faculty of Pharmaceutical Science, Associate Professor (00314427)
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|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥4,230,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥630,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
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| Keywords | Small GTPase / Proton pump / Acidification / V-ATPase / pH sensor / Membrane traffick |
|---|
| Research Abstract |
The small GTPase Arf6 and ARNO are involved in regulation of endocytosis. We demonstrated the crucial role of acidification of early endosomes and V-ATPase/ARNO/Arf6 interactions in the regulation of the degradative pathway during endocytosis. We also found that V-ATPase with the α3 isoform is highly expressed in pancreatic islet, and localized to membranes of insulin-containing secretory granules in b cells. oc/oc mice, which have a null mutation at the α3 locus, exhibited a reduced level of insulin in the blood, even with high glucose administration. These results suggest that the α3 isoform of V-ATPase has regulatory functions in ,exocytosis of insulin secretion.
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