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Elucidation of the link between the control of cell-cell adhesion by regulations of cell polarity and cell proliferation and differentitation

Research Project

Project/Area Number 18570142
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

IZAWA Ichiro  Aichi Cancer Center Research Institute, Division of Biochemistry, Section Head (20311441)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSignal transduction / Cancer / Development / Brain / Cell polarity
Research Abstract

LAP(leucine-rich repeats(LRR) and PSD-95/Dlg/ZO-1(PDZ)) family proteins, including Scribble, LET-413, ERBIN, Densin-180, and Lano, are involved in the regulation of cell polarity. The LRR domains of LAP proteins were reported to mediate their basolateral membrane localization and to be essential for their function. To further dissect the mechanism of the plasma membrane localization of ERBIN, we introduced various mutants of ERBIN into cultured cells and observed the intracellular localization. When an LRR domain mutant lacking amino acid residues 1-32 at the amino(N) terminal region was overexpressed in cells, the mutant did not localize at the plasma membrane, but localized in the cytoplasm. We found that cysteines 14 and 16 at the N-terminal region of ERBIN are in vivo palmitoylated. Overexpression of the mutants in which cysteine 14 and/or cysteine 16 were changed to serines did not localize at the plasma membrane, indicating that the palmitoylation of ERBIN is necessary for its plasma membrane localization. The overexpressed 1-196 amino acids fragment of ERBIN, which lacked the latter half of LRR, was palmitoylated but did not localize at the plasma membrane. These results suggest that both palmitoylation and LRR are required for the plasma membrane localization of ERBIN.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 2006 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (4 results) Book (1 results)

  • [Journal Article] Non-pathogenic protein aggregates in skeletal muscle in MLF1 transgenic mice.2008

    • Author(s)
      Li, Z.F.
    • Journal Title

      J.Neurol.Sci. 264

      Pages: 77-88

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Non-pathogenic piotein aggregates in skeletal muscle in MLF1 transgenic mice.2008

    • Author(s)
      Li, Z.F., Wu, X., Jiang, Y., Liu, J., Wu, C., Inagaki, M., Izawa. I., .Mizisin, A.P., Engvall, E. and Shelton, G.D.
    • Journal Title

      J. Neurol. 264

      Pages: 77-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Non-pathogenic protein aggregates in skeletal muscle in MLF1 transgenic mice.2008

    • Author(s)
      Li, Z.F.
    • Journal Title

      J. Neurol. Sci. 264

      Pages: 77-88

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vimentin-Ser-82 as a memory phosphorylation site in astrocutes.2006

    • Author(s)
      Oguri, T.
    • Journal Title

      Genes Cells 11

      Pages: 531-540

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes.2006

    • Author(s)
      Oguri, T., Inoko, A., Shima, H., Izawa, I., Arimura, N., Yamaguchi, T., Inagaki, N., Kaibuchi, K., Kikuchi, K. and Inagaki, M.
    • Journal Title

      Genes Cells 11

      Pages: 531-540

    • NAID

      120000961744

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes2006

    • Author(s)
      Oguri, T., et al.
    • Journal Title

      Genes Cells 11

      Pages: 531-540

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Palmitoylation of ERBIN is required for its plasma membrane localization.

    • Author(s)
      Izawa, I.
    • Journal Title

      Genes Cells (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Palmitoylation of ERBIN is required for its plasma membrane localization.

    • Author(s)
      Izawa. I,. Nishizawa, M., Hayashi, Y., and Inagaki, M.
    • Journal Title

      Genes Cells (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Palmitoylation of ERBIN is required for its plasma membrane localization.

    • Author(s)
      Izawa, I.
    • Journal Title

      Genes Cells (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Palmitoylation is required for the plasma membrane localization of ERBIN.2007

    • Author(s)
      Izawa, I.
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2007-10-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Palmitoylation is required for the plasma localization of ERBIN.2007

    • Author(s)
      Izawa. I., Nishizawa, M., Inagaki, M.
    • Organizer
      65th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      2007-10-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 細胞極性制御分子ERBINの形質膜移行メカニズムの解析2006

    • Author(s)
      井澤一郎
    • Organizer
      第65回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2006-09-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of the mechanism of the plasma membrane targeting of ERBIN, a cell polarity regulator.2006

    • Author(s)
      Izawa. I., Nishizawa, M., Inagaki, M.
    • Organizer
      65th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      2006-09-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 実験医学 第24巻第13号「形と運動を司る細胞のダイナミクス」第1章 6.中間径フィラメントの構築制御とシグナルクロストーク2006

    • Author(s)
      井澤一郎
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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