Elucidation of the link between the control of cell-cell adhesion by regulations of cell polarity and cell proliferation and differentitation
| Project/Area Number |
18570142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
IZAWA Ichiro Aichi Cancer Center Research Institute, Division of Biochemistry, Section Head (20311441)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Signal transduction / Cancer / Development / Brain / Cell polarity |
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| Research Abstract |
LAP(leucine-rich repeats(LRR) and PSD-95/Dlg/ZO-1(PDZ)) family proteins, including Scribble, LET-413, ERBIN, Densin-180, and Lano, are involved in the regulation of cell polarity. The LRR domains of LAP proteins were reported to mediate their basolateral membrane localization and to be essential for their function. To further dissect the mechanism of the plasma membrane localization of ERBIN, we introduced various mutants of ERBIN into cultured cells and observed the intracellular localization. When an LRR domain mutant lacking amino acid residues 1-32 at the amino(N) terminal region was overexpressed in cells, the mutant did not localize at the plasma membrane, but localized in the cytoplasm. We found that cysteines 14 and 16 at the N-terminal region of ERBIN are in vivo palmitoylated. Overexpression of the mutants in which cysteine 14 and/or cysteine 16 were changed to serines did not localize at the plasma membrane, indicating that the palmitoylation of ERBIN is necessary for its plasma membrane localization. The overexpressed 1-196 amino acids fragment of ERBIN, which lacked the latter half of LRR, was palmitoylated but did not localize at the plasma membrane. These results suggest that both palmitoylation and LRR are required for the plasma membrane localization of ERBIN.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Non-pathogenic piotein aggregates in skeletal muscle in MLF1 transgenic mice.2008
Author(s)
Li, Z.F., Wu, X., Jiang, Y., Liu, J., Wu, C., Inagaki, M., Izawa. I., .Mizisin, A.P., Engvall, E. and Shelton, G.D.
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Journal Title
J. Neurol. 264
Pages: 77-86
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes.2006
Author(s)
Oguri, T., Inoko, A., Shima, H., Izawa, I., Arimura, N., Yamaguchi, T., Inagaki, N., Kaibuchi, K., Kikuchi, K. and Inagaki, M.
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Journal Title
Genes Cells 11
Pages: 531-540
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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