Structure Formation of the Neck which Links the Head and the Tail of Bacteriophage

• Project/Area Number: 18570147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: ARISAKA Fumio Tokyo Institute of Technology, GRADUATE SCHOOL OF BIOSCIENCE AND BIOTECHNOROGY, ASSOCIATE PROFESSOR (80133768)
• Co-Investigator(Kenkyū-buntansha): KANAMARU Shuji TOKYO INSTITUTE OF TECHNOLOGY, GRADUATE SCHOOL OF BIOSCIENCE AND BIOTECHNOROGY, ASSISTANT PROFESSOR (50376951)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: bacteriophage / molecular assembly / analytical ultracentrifugation / multiangle laser light scattering / protein interactions / neck protein / X-ray crystallography / virus

Research Abstract

During the assembly of phage T4, the neck is formed to block the DNA leakage and to prepare for the tail association after DNA packaging into the head is completed. The neck is composed of gp13 (gp: gene product) and gp14 each consists of 309 and 256 amino acid residues with the molecular weight of 41,226 and 32,868, respectively. In order to elucidate the structure and the subunit arrangement of the neck, genes coding for the two proteins were cloned by PCR method, an overexpression system was constructed and the genes were induced by IPTG. The two proteins were purified by ammonium sulfate fractionation, ion exchange chromatography, gen filtration as a single band in SDS-PAGE. Far-ultraviolet circular dichroism spectra of the two proteins indicated that gp13 is rich in α-helix, whereas gp13 is rich in β-sheet. On the other hand, sedimentation velocity indicated that both proteins are monomers with the sedimentation coefficient of 2.80S and 2.00S, respectively and that the frictional ratios were 1.22 and 1.73, respectively, which indicated that gp14 is more elongated than gp13. The two proteins did not interact each other under normal physiological conditions, but it was found that the two proteins formed a specific complex with the sedimentation coefficient of 16.6S. The complex had the molecular weight of 497,000 which together with the molar ratio of 2:1 based on SDS-PAGE indicated that the complex is a hetero 15-mer, (gp 13)10(gp 14)5. This complex is considered to be identical with the complex which was observed when the two proteins were co-expressed. Electron microscopic observations revealed that the complex formed a ring with the diameter of 156 Å which coincided with the electron density of the three-dimensional image of the neck reconstructed from electron micrograph.

Research Products

• [Journal Article] The motor protein prestin is a bullet-shaped molecule with inner cavities (2008)
  • Author(s): Kazuhiro Mio, Yoshihiro Kubo, Toshihiko Ogura, Tomomi Yamamoto, Fumio Arisaka and Chikara Sato
  • Journal Title: J. Biol. Chem. 283(2) Pages: 1137-45
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular design of heteroprotein assemblies providing a bionanocupas a chemical reactor (2008)
  • Author(s): Koshiyama T, Ueno T, Kanamaru S, Nagano S, Shiro Y, Arisaka F, Watanabe Y
  • Journal Title: Small 4 Pages: 50-54
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Activity-dependent neurotrophic factor, ADNF, detemines the structure characteristics of Colivelin, a fusion protein of ADNF9 and Humaninanalog (2008)
  • Author(s): Tsutomu Arakawa, Takako Niikura, Fumio Arisaka and Yoshiko Kita
  • Journal Title: J. Peptide Science 14 Pages: 631-636
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Fumio Arisaka and Chikara Sato : The motor protein prestin is a bullet-shaped molecule with inner cavities. (2008)
  • Author(s): Kazuhiro, Mio, Yoshihiro, Kubo, Toshihiko, Ogura, Tomomi, Yamamoto
  • Journal Title: J.Biol.Chem. 283(2) Pages: 1137-45
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor (2008)
  • Author(s): Koshiyama, T, Ueno T, Kanamaru S, Nagano, S, Shiro Y, Arisaka, F, Watanabe Y
  • Journal Title: Small 4 Pages: 50-54
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Takako Niikura, Fumio Arisaka,and Yoshiko Kita : Activity-dependent neurotrophic factor,ADNF,determines the structure characteristis of Colivelin,a fusion protein of ADNF9 and Humanin analog. (2008)
  • Author(s): Tsutomu, Arakawa
  • Journal Title: J. Peptide Science 14 Pages: 631-636
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor (2008)
  • Author(s): Koshiyama T, Ueno T, Kanamaru S, Nagano S, Shiro Y, Arisaka F, Watanabe Y
  • Journal Title: Small. 4 Pages: 50-54
  • Peer Reviewed
• [Journal Article] From structure of the complex to understanding of the biology (2007)
  • Author(s): Rossmann, M. G., Arisaka, F., Battisti, A. J., Bowman, V. D., Chipman, P. R., Fokine, A., Hafenstein, S., Kanamaru, S., Kostyuchenko, V. A., Mesyanzhinov, V. V., Shneider, M. M., Morais, M. C., Leiman, P. G., Palermo, L. M., Parrish, C. R., and Xiao, C.
  • Journal Title: Acta Cryst. D63 Pages: 9-16
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing (2007)
  • Author(s): Abe M, Ohno S, Yokogawa T, Nakanishi T, Arisaka F, Hosoya T, Hiramatsu T, Suzuki M, Ogasawara T, Sawasaki T, Nishikawa K, Kitamura M, Hori H, Endo Y.
  • Journal Title: Proteins 67 Pages: 643-652
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Changes in Glycosylation of Vittonectin Modulate Multimerization and Collagen Binding during Liver Regeneration (2007)
  • Author(s): Sano K, Asanuma-Date K, Arisaka F, Hattori S, Ogawa H.
  • Journal Title: Glycobiology 17 Pages: 784-794
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The neck of bacteriophage T4 is a ring-like strucure formed by a hetero-oligomer of gp13 and gp14 (2007)
  • Author(s): Akhter, T., Zhao, L., Kohda, A., Mio, K., Kanamaru, S. & Arisaka, F.
  • Journal Title: Biochim. Biophys. Acta 1774 Pages: 1036-1043
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Reversible and Irreversible Coiled Coils in the Stalk Domain of ncd Motor Protein (2007)
  • Author(s): Makino T, Morii H, Shimizu T, Arisaka F, Kato Y, Nagata K, and Tanokura M
  • Journal Title: Biochemistry 46 Pages: 9523-9532
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Aggregation Analysis of Therapeutic Proteins, Part 2 (2007)
  • Author(s): Arakawa T, John S. Philo, Ejima D, Tsumoto K, and Arisaka F.
  • Journal Title: Ultracentrifugation and Dynamic Light Scattering Bio Process International 5 Pages: 36-50
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] An arthropod cuticular chitin-binding protein endows injured sites with transglutaminase-dependent mesh (2007)
  • Author(s): Matsuda Y, Koshiba T, Osaki T, Suyama H, Arisaka F, Toh Y, Kawabata SI
  • Journal Title: J. Biol. Chem. 282 Pages: 37316-37324
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Stmcture Analysis of Activity-dependent Neurotrophic Factor 9 by Circular Dichroism and Sedimentation Equilibrium (2007)
  • Author(s): Arakawa T, Niikura T, Tajima H, Arisaka F, Kita Y.
  • Journal Title: J. Mol. Neurosci. 33 Pages: 262-267
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] ウイルスに見られる新しいβ構造モチーフ (2007)
  • Author(s): 有坂文雄、 金丸周司
  • Journal Title: 日本結晶学会誌 49 Pages: 161-170
  • NAID: 10019748100
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] バクテリオファージの構造生物学 (2007)
  • Author(s): 有坂文雄、 金丸周司
  • Journal Title: 蛋白質 核酸 酵素 52 Pages: 1069-1074
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Arisaka, F., Battisti, A. J., Bowman, V. D., Chipman, P. R. Fokine, A., Hafenstein, S., Kanamaru, S., Kostyuchenko, V.A., Mesyanzhinov, V. V., Shneider, M. M., Morais, M.C., Leiman, P.G., Palermo, L. M., Parrish, C.R., and Xiao, C. From structure of the complex to understanding of the biology. (2007)
  • Author(s): M. G., Rossmann
  • Journal Title: Acta Cryst D63 Pages: 9-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing (2007)
  • Author(s): M, Abe, S, Ohno, T, Yokogawa, T, Nakanishi, F, Arisaka, T, Hosoya, T, Hiramatsu, M, Suzuki, T, Ogasawara, T, Sawasaki, K, Nishikawa, M, Kitamura, H, Hori, Y., Endo
  • Journal Title: Proteins 67 Pages: 643-652
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in Glycosylation of Vittonectin Modulate Multimerization and Collagen Binding during Liver Regeneration. (2007)
  • Author(s): K, Sano, K, Asanuma-Date, F, Arisaka, S, Hattori, H., Ogawa
  • Journal Title: Glycobiology 17 Pages: 784-794
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zhao, L., Kohda, A., Mio, K., Kanamaru, S. & Arisaka, F. The neck of bacteriophage T4 is a ring-like structure formed by a hetero-oligomer of gp13 and gp14. (2007)
  • Author(s): T., Akhter
  • Journal Title: Biochim.Biophys Acta 1774 Pages: 1036-1043
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reversible and Irreversible Coiled Coils in the Stalk Domain of ncd Motor Protein (2007)
  • Author(s): T, Makino, H, Morii, T, Shimizu, F, Arisaka, Y, Kato, K, Nagata, M. Tanokura
  • Journal Title: Biochemistry 46 Pages: 9523-9532
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aggregation Aspects and Techniques for Assessment, Part 2 Analytical Ultracentrifugation and Dynamic Light Scattering. (2007)
  • Author(s): T, Arakawa, S., John, Philo, D, Ejima, K, Tsumoto, F, Arisaka
  • Journal Title: BioProcess International 5(4) Pages: 36-50
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kawabata SI An arthropod cuticular chitin-binding protein endows injured sites with transglutaminase-dependent mesh. (2007)
  • Author(s): Y, Matsuda, T, Koshiba, T, Osaki, H, Suyama, F, Arisaka, Y, Toh
  • Journal Title: J.Biol.Chem. 282 Pages: 37316-37324
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure Analysis of Activity-dependent Neurotrophic Factor 9 by Circular Dichroism and Sedimentation Equilibrium. (2007)
  • Author(s): T, Arakawa, T, Niikura, H, Tajima, F, Arisaka, Y., Kita
  • Journal Title: J.Mol.Neurosci. 33 Pages: 262-267
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fumio Arisaka Novel β -structure Motifs from Viruses and Bacteriophages (2007)
  • Author(s): Shuji, Kanamaru
  • Journal Title: J. Cryst. Soc. Japan 49 Pages: 161-170
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fumio Arisaka Structural biology of bacteriophage (2007)
  • Author(s): Shuji, Kanamaru
  • Journal Title: Prot.Nuc.Enz. 52 Pages: 1069-1074
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 「Tokoton-Yasashii Tanpakushitsu-noHon」 Ed. Grad Sch of Biosci. & Biotech, Tokyo Institute of Technology (2007)
  • Author(s): Fumio, Arisaka
  • Journal Title: Nikkan Kogyo Shimbun Co. Pages: 12-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fumio Arisaka Analysis of Protein Interactions by Analytical Ultracentrifugation 「Handbook of Analysis of Molecular Interactions」 Isobe, Nakayama, Ito eds. (2007)
  • Author(s): Fumio, Arisaka
  • Journal Title: Youdo-sha Pages: 151-157
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing (2007)
  • Author(s): Abe M, Ohno S, Yokogawa T, Nakanishi T, Arisaka F, Hosoya T, Hiramatsu T, Suzuki M, Ogasawara T, Sawasaki T, Nishikawa K, Kitmura M, Hori H, Endo Y.
  • Journal Title: Proteins. 2007 May 15; 67 Pages: 643-652
  • Peer Reviewed
• [Journal Article] CHANGES IN GLYCOSYLATION OF VITRONECTIN MODULATE MULTIMERIZATION AND COLLAGEN BINDING DURING LIVER REGENERATION (2007)
  • Author(s): Sano K, Asanuma-Date K, Arisaka F, Hattori S, Ogawa H.
  • Journal Title: Glycobiology. 2007 17 Pages: 784-794
  • Peer Reviewed
• [Journal Article] The neck of bacteriophage T4 is a ring-like structure formed by a hetero-oligomer of gp13 and gp14. (2007)
  • Author(s): Akhter, T., Zhao, L., Kohda, A., Mio, K., Kanamaru, S. & Arisaka, F.
  • Journal Title: Biochim Biophys Acta 1774 Pages: 1036-1043
  • Peer Reviewed
• [Journal Article] Aggregation Aspects and Techniques for Assessment, Part 2Analytical (2007)
  • Author(s): Arakawa T, John S. Philo, Ejima D, Tsumoto K, and, Arisaka F.
  • Journal Title: Ultracentrifugation and Dynamic Light Scattering BioProcess International 5 Pages: 36-50
  • Peer Reviewed
• [Journal Article] An arthropod cuticular chitin-binding protein endows injured sites with transglutaminase-dependent mesh. (2007)
  • Author(s): Matsuda Y, Koshiba T, Osaki T, Suyama H, Arisaka F, Toh Y, Kawabata SI
  • Journal Title: J Biol Chem 282 Pages: 37316-37324
  • Peer Reviewed
• [Journal Article] Structure Analysis of Activity-dependent Neurotrophic Factor9 by Circular Dichroism and Sedimentation Equilibrium. (2007)
  • Author(s): Arakawa T, Niikura T, Tajima H, Arisaka F, Kita Y.
  • Journal Title: J Mol Neurosci 33 Pages: 262-267
  • Peer Reviewed
• [Journal Article] Reversible and Irreversible Coiled Coils in the Stalk Domain of ncd Motor protein (2007)
  • Author(s): Makino T, Morii H, Shimizu T, Arisaka F, Kato Y, Nagata K, and Tanokura M
  • Journal Title: Biochemistry 46 Pages: 9523-9532
  • Peer Reviewed
• [Journal Article] ウイルスに見られる新しいβ構造モチーフ. (2007)
  • Author(s): 金丸周司、有坂文雄
  • Journal Title: 日本結晶学会誌 49 Pages: 161-170
  • NAID: 10019748100
• [Journal Article] バクテリオファージの構造生物学 (2007)
  • Author(s): 金丸周司、有坂文雄
  • Journal Title: 蛋白質 核酸 酵素 52 Pages: 1069-1074
• [Journal Article] From structure of the complex to understanding of the biology. (2007)
  • Author(s): Rossmann, M.G., Arisaka, E., Battisti, A.J., Bouman, V.D., Chipman, P.R., Fokine, A., Hafenstein, S., Knamaru, S., Kostyuchenko, V.A., Mesyanzhinov, V.V., Shineider, M..M., Morais, M.C., Leiman, P.G., Palermo, L.M., Parrish,C.R., Xiao,C.
  • Journal Title: Acta Cryst d63 Pages: 9-16
• [Journal Article] Molecular Mechanism for Pterin-Mediated Inactivation of Tyrosine Hydroxylase: Formation of Insoluble Aggregates of Tyrosine Hydroxylase (2006)
  • Author(s): Urano, F., Hayashi, N., Arisaka, F., Kurita, H., Murata, S., and Ichinose, H.
  • Journal Title: J. Biochem. 139 Pages: 625-635
  • NAID: 10018845291
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Construction of a Ball-and-Spike Protein Supramolecule (2006)
  • Author(s): Sugimoto, K., Kanamaru, S., Iwasaki, K., Arisaka, F., Yamashita, I.
  • Journal Title: Angew Chem Int Ed Engl. Apr 21. 45(17) Pages: 2725-2728
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Bionanotube Tetrapod Assembly by In Situ Synthesis of a Gold Nanocluster with(gp5-His6)3 from Bacteriophage T4 (2006)
  • Author(s): Ueno, T., Koshiyama, T., Tsuruga, T., Goto, T., Kanamaru, S., Arisaka, F., and Watanabe, Y.
  • Journal Title: Angew. Chem. Int. Ed. 45 Pages: 4508-4512
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Association and dissociation of the cell puncturing complex of bacteriophage T4 is controlled by both pH and temperature (2006)
  • Author(s): Sarkar, S. K., Takeda, Y., Kanamaru, S., Arisaka. F.
  • Journal Title: Biochim. Biophys. Acta, 1764 Pages: 1487-1492
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The Asymmetric IscA Homodimer with and Exposed[2Fe-2S]Cluster Suggests the Structural Basis of the Fe-S Cluster Biosynthetic Scaffold (2006)
  • Author(s): Morimoto, K., Yamashita, E., Kondou, Y., Lee, Soo Jae., Arisaka, F., Tsukihara, T., and Nakai, M.
  • Journal Title: J. Mol. Biol. 360, Pages: 117-132
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Thioredoxin-hl reduces and reactivates the oxidized cytosolic malate dehydeogenase dimer in higher plants (2006)
  • Author(s): Hara, S., Motohashi, K., Arisaka, F., Romano, P. G. N., Hosoya-Matsuda, N., Kikuchi, N., Fusada, N., and Hisabori, T.
  • Journal Title: J. Biol. Chem. 281(43) Pages: 32065-32071
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Aggregation Analysis of Therapeutic Proteins, Part 1. (2006)
  • Author(s): Arakawa, T., Philo, J. S., Ejima, D., Tsumoto, K., and Arisaka, F.
  • Journal Title: Bio Process International 4(10) Pages: 32-42
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] F., Hayashi, N., Arisaka, F., Kurita, H., Murata, S., and Ichinose, H., Molecular Mechanism for Pterin-Mediated Inactivation of Tyrosine Hydroxylase : Formation of Insoluble Aggregates of Tyrosine Hydroxylase. (2006)
  • Author(s): F., Urano
  • Journal Title: J. Biochem 139 Pages: 625-635
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kanamaru, S., Iwasaki, K., Arisaka, F., Yamashita, I. : Construction of a Ball-and-Spike Protein Supramolecule. (2006)
  • Author(s): K., Sugimoto
  • Journal Title: Angew. Chem. Int. Ed. 45 Pages: 2725-2728
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Koshiyama, T., Tsuruga, T., Goto, T., Kanamaru, S., Arisaka, F., and Watanabe, Y. : Bionanotube Tetrapod Assembly by In Situ Synthesis of a Gold Nanocluster with (gp5-His6)3 from Bacteriophage T4. (2006)
  • Author(s): T., Ueno
  • Journal Title: Angew. Chem. Int. Ed. 45 Pages: 4508-4512
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Takeda, Y., Kanamaru, S., Arisaka, F. : Association and dissociation of the cell puncturing complex of bacteriophageT4 is controlled by both pH and temperature. (2006)
  • Author(s): S.K., Sarkar
  • Journal Title: Biochim.Biophys.Acta 1764 Pages: 1487-1492
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Yamashita,E., Kondou, Y., Lee, Soo Jae., Arisaka, F., Tsukihara, T., and Nakai, M. : The Asymmetric IscA Homodimer with and Exposed [2Fe-2S] Cluster Suggests the Structural Basis of the Fe-S Cluster Biosynthetic Scaffold. (2006)
  • Author(s): K., Morimoto
  • Journal Title: J.Mol.Biol. 360 Pages: 117-132
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Motohashi, K., Arisaka,F., Romano,P.G.N., Hosoya-Matsuda, N., Kikuchi, N., Fusada, N., and Hisabori, T. : Thioredoxin-h1 reduces and reactivates the oxidized cytosolic malate dehydeogenase dimer in higher plants. (2006)
  • Author(s): S., Hara
  • Journal Title: J.Biol.Chem. 281 43) Pages: 32065-32071
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Philo, J. S., Ejima, D., Tsumoto, K., and Arisaka, F. Aggregation Analysis of Therapeutic Proteins, Part 1 (2006)
  • Author(s): T., Arakawa
  • Journal Title: BioProcess Int. 4(10) Pages: 32-42
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aggregation Aspects and Techniques for Assessment,Part 1. (2006)
  • Author(s): Arakawa, T., Philo, J.S., Ejima, D., Tsyumoto, K., Arisaka,F.
  • Journal Title: Bioprocess International. 4, Pages: 32-42
• [Journal Article] Thiredoxin-h1 reduces and reactivates the oxidized cytosolic malate dehydeogenase dimer in higher plants. (2006)
  • Author(s): Hara, S., Motohashi, K, Arisaka, F., Oomano, P.G.N., Hosoya-Matsuda, N., Kikuchi, N., Fusada, N., Hisabori,T
  • Journal Title: J.Boil.chem. 281(43) Pages: 32065-71
• [Journal Article] The Asymmetric IscA Homodemer with and Exposed[2Fe-2S] Cluster Suggests the Structural Basis of the Fe-S Cluster Biosynthetic Scaffold (2006)
  • Author(s): Morimoto, K., Yamashita, E., Kondou, Y., Lee, Soo Jae., Arisaka, E., Tsukihara, T., Nakai,M.
  • Journal Title: J.Mol.Biol. 360, Pages: 117-132
• [Journal Article] Bionanotube Tetrapod Assembly by In Situ Synthesis of a Gold Anocluster with(gp5-His6)3 from Bacteriophage T4. (2006)
  • Author(s): Ueno, T., Koshiyama, T., Tsuruga, T., Goto, T., Kanamaru, S., Arisaka, F., Watanabe,Y.
  • Journal Title: Angew.Chem.Int.Ed. 45 Pages: 4508-4512
• [Journal Article] Molecular Mechanism for Pterin-Mediated Inacivation of Tyrosine Hydroxylase : Formation of Insoluble Aggregates of Tyrosine Hydroxylase. (2006)
  • Author(s): Urano, F., Hayashi, N., Arisaka, F., Kurita, H., Murata, S., Ichinose,H.
  • Journal Title: J.Biochem. 139 Pages: 625-635
• [Journal Article] Construction of a Ball-and-Spike Protein Supramolecule. (2006)
  • Author(s): Sugimoto, K., Kanamaru, S., Iwasaki, K., Ariska, E., Yamashita,I.
  • Journal Title: Angew Chem Int Ed Engle.Apr21. 45(17) Pages: 2725-8
• [Journal Article] Association and dissociation of the cell puncturing complex of bacteriophageT4 is controlled by both pH and temperature. (2006)
  • Author(s): Sarkar, S.K., Takeda, Y., Kanamaru.S., Arisaka, F.
  • Journal Title: Biochim.Biophys.Acta, 1764 Pages: 1487-1492
• [Presentation] T4ファージの構造生物学と感染機構 (2008)
  • Author(s): 金丸周司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structural biology and the mechanism of bacteriophage T4. (2008)
  • Author(s): Shuji, Kanamaru
  • Organizer: Ann. meeting of Japan Soc. Bacteriol. Kyoto
  • Place of Presentation: Kyoto
  • Year and Date: 2008-03-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] T4ファージロングテイルファイバーgp34の末端欠損タンパク質の性状解析 (2007)
  • Author(s): Donny Sunanda、 金丸周司有坂文雄
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] バクテリオファージT4の感染初期過程 (2007)
  • Author(s): 二島渉、金丸周司、有坂文雄北尾彰朗
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ダイニンの構造的最小単位の同定 (2007)
  • Author(s): 沼田直巳、Roberts Anthony, 大倉玲子、有坂文雄、昆隆英Knight Peter、Burgess Stan、須藤和夫
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Crystallization of the C-terminal-truncated mutant proteins of gp34, the proximal half tail fiber of bacteriophage T4 (2007)
  • Author(s): Sunanda, Donny, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 45th annual meeting of the Biophysical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The initial steps of infection of bacteriophage T4. (2007)
  • Author(s): Wataru, Nishima, Shuji, Kanamaru, Fumio, Arisaka, Akira, Kitao
  • Organizer: The 45th annual meeting of the Biophysical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Are six AAA+ modules sufficient to form the ring of dynein? (2007)
  • Author(s): Naomi, Numata, Roberts, Anthony, Reiko, Ogura, Fumio, Arisaka, Takahide, Kon, Knight, Peter, Burgrss, Stan, Kazuo, Sutoh
  • Organizer: The 45th annual meeting " The Biophysical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 長さ・太さの制御可能な三本鎖βヘリックスタンパク質の作製 (2007)
  • Author(s): 寺内允、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージ基盤蛋白質gp48の可溶化と性状解析 (2007)
  • Author(s): 伊藤麻紗子、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] PP01ファージの尾繊維タンパク質gp38,gp37の解析 (2007)
  • Author(s): 堀越祐介、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Foldon fusion to the C-terminal-truncated mutant Proteins of gp34, the Proximal half tail fiber of bacteriophage T4 to facilitate trimer for matron. (2007)
  • Author(s): Donny Sunanda、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression and Characterization of the tail fiber proteins gp38 and gp37 of bacteriophage PPO1. (2007)
  • Author(s): Yusuke, Horikosh, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 4th symposium "Biological Supra-molecular Structure"
  • Place of Presentation: Osaka
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction and overexpression of the length- and width- changeable Triple-stranded b-helix. (2007)
  • Author(s): Makoto, Terauchi, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 4th symposium "Biological Ssupra-molecular Structure"
  • Place of Presentation: Osaka
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Solubilization and characterization of gp48, a baseplate protein, of bacteriophage T4. (2007)
  • Author(s): Masako, Ito, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 4th symposium "Biological Ssupra-molecular Structure"
  • Place of Presentation: Osaka
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Foldon fusion to the C-terminal-truncated mutant proteins of gp34, the proximal half tail fiber of bacteriophage T4 helps Gp34N 325F folds into native structure.` (2007)
  • Author(s): Donny, Sunanda, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 4th symposium "Biological Ssupra-molecular Structure"
  • Place of Presentation: Osaka
  • Year and Date: 2007-12-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] T4ファージ基盤構成蛋白質gp7,gp10,gp11の相互作用部位の同定 (2007)
  • Author(s): 中尾朋子、長尾達也、金丸周司, 有坂文雄
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会横浜
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージ基盤終結蛋白質gp48,gp54の大量発現系の構築と性状解析 (2007)
  • Author(s): 伊藤麻紗子、金丸周司、有坂文雄
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会横浜
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] An arthropod cuticular chitin-binding protein endows injured sites with transglutaminase-dependent mesh (2007)
  • Author(s): 松田泰幸、小柴琢巳、尾崎司、陶山春香、有坂文雄、藤義博、川畑俊一郎
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会 BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Identification of the interaction sites of gp7, gp10, gp11, the baseplate proteins of bacteriophage T4. (2007)
  • Author(s): Tomoko, Nakao, Tatsuya, Nagao, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: BMB2007 Biochem. & Mol. Biol.
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction of overexpression system of gene 48 and gene 54, the completion protein of the baseplate of bacteriopahge T4. (2007)
  • Author(s): Masako, Ito, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: BMB2007 Biochem. & Mol. Biol.
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] An arthropod cuticular chitin-binding protein endows injured sites with transglutaminase-dependent mesh (2007)
  • Author(s): Yasuyuki, Matsuda, Takumi, Koshiba, Tskasa, Osaki, Haruka, Toyama, Fumio, Arisaka,Yoshihiro, Fuji, Syunishiro, Kawabata
  • Organizer: BMB2007 Biochem. & Mol. Biol.
  • Place of Presentation: Yokohama
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] T4ファージ基盤蛋白質群の断片化複合体の結晶化の試み (2007)
  • Author(s): 中尾朋子、長尾達也、金丸周司, 有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ.
  • Place of Presentation: 熱海
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 可変長人工βヘリックス構造の性状解析 (2007)
  • Author(s): 寺内允、金丸周司、有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ.
  • Place of Presentation: 熱海
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージ基盤終結蛋白質gp48、gp54の大量発現系の構築と性状解析 (2007)
  • Author(s): 伊藤麻紗子、金丸周司、有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ
  • Place of Presentation: 熱海.
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Crystallization of the C-terminal-truncated mutant proteins of gp34, the proximal half tail fiber of bactedohae T4 (2007)
  • Author(s): Donny Sunanda、金丸周司、有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ
  • Place of Presentation: 熱海
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4類縁ファージのgp5ホモログ蛋白質の性状解析と結晶化 (2007)
  • Author(s): 根本舞、油井孔兵、金丸周司, 有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ
  • Place of Presentation: 熱海
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Progress report on the crystallization of complex that had been rendered limited proteolysis. (2007)
  • Author(s): Tomoko, Nakao, Tatuya, Nagao, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 3rd workshop on "Biological Supra-molecular Structure"
  • Place of Presentation: Atami
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of length-variable β-helix. (2007)
  • Author(s): Makoto, Terauchi, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 3rd workshop "Biological Supra-molecular Structure"
  • Place of Presentation: Atami
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction of overexpression system of gene 48 and gene 54, the completion protein of the baseplate of bacteriopahge T4. (2007)
  • Author(s): Masako, Ito, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 3rd workshop "Biological Supra-molecular Structure"
  • Place of Presentation: Atami
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Crystallization of the C-terminal-truncated mutant proteins of gp34, the proximal half tail fiber of bacteriophage T4 (2007)
  • Author(s): Donny, Sunanda, Shuji, Kana,maru, Fumio, Arisaka
  • Organizer: The 3rd workshop "Biological Ssupra-molecular Structure"
  • Place of Presentation: Atami
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization and crystallization of the T4 gp5 homolog of T4-related phages (2007)
  • Author(s): Mai, Nemoto, Kohei, Aburai, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: The 3rd workshop "Biological Ssupra-molecular Structure"
  • Place of Presentation: Atami
  • Year and Date: 2007-07-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] カブトガニ外皮タンパク質(カラキシン)は、トランスグルタミナーゼにより架橋され網目状繊維を形成する (2007)
  • Author(s): 松田泰幸、小柴琢己、尾崎司、有坂文雄、川畑俊一郎
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] バクテリオファージT4尾部の膜貫通機構 (2007)
  • Author(s): 二島渉、金丸周司、有坂文雄、北尾彰朗
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Consruction and purification of N terminal of the proximal long tail fiber, gp34, Of Bacteriophage T4 (2007)
  • Author(s): Donny Sunanda、金丸周司, 有坂文雄
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージ基盤構成蛋白質群のドメイン構造と相互作用部位の同定 (2007)
  • Author(s): 中尾朋子、長尾達也、金丸周司, 有坂文雄
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Fumio Arisaka Syunichiro Kawabata : An arthropod cuticular chitin-binding protein exists as an oligomer and endows transglutaminase-dependent mesh at injured sites. (2007)
  • Author(s): Yasuyuki, Matuda, Takumi, Kosiba, Tukasa, Ozaki
  • Organizer: The 7th Ann. Meeting of the Prot. Sci. Soc. of Japan
  • Place of Presentation: Sendai
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Penetration mechanism of bacteriophage T4 tail. (2007)
  • Author(s): Wataru, Nishima,Shuji, Kanamaru, Fumio, Arisaka, Akio, Kitao
  • Organizer: The 7th Ann. Meeting of the Prot. Sci. Soc. of Japan
  • Place of Presentation: Sendai
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fumio Arisaka Consruction and purification of N terminal of the proximal long tail fiber, gp34, of Bacteriophage T4. (2007)
  • Author(s): Donny, Sunanda, Shuji, Kanamaru
  • Organizer: The 7th Ann. Meeting of the Prot. Sci. Soc. of Japan
  • Place of Presentation: Sendai
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Tatuya Nagao,huji Kanamaru, Fumio Arisaka Damain interaction analyses of the baseplate proteins,gp7,gp10 and gp11,of bacteriophage T4 (2007)
  • Author(s): Tomoko, Nakao
  • Organizer: The 7th Ann. Meeting of the Prot. Sci. Soc. of Japan
  • Place of Presentation: Sendai
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 人工三本鎖βヘリックスを用いたナノチューブの作製 (2007)
  • Author(s): 金丸周司
  • Organizer: バイオ・ナノ分子自己組織化ワークショップ
  • Place of Presentation: 静岡
  • Year and Date: 2007-02-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Construction of the artificial triple-stranded b-helix. (2007)
  • Author(s): Shuji, Kanamaru
  • Organizer: Workshop on the self-organization of bio-nano
  • Place of Presentation: Sizuoka
  • Year and Date: 2007-02-09
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] バクテリオファージT4尾部の膜貫通機構 (2007)
  • Author(s): 二島渉、金丸周司、有坂文雄、北尾彰朗
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台国際センター
• [Presentation] Consruction and purification of N terminal of the proximal long tail fiber, gp34, of Bacteriophage T4 (2007)
  • Author(s): Donny Sunanda、金丸周司, 有坂文雄
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台国際センター
• [Presentation] T4ファージ基盤構成蛋白質群のドメイン構造と相互作用部位の同定 (2007)
  • Author(s): 中尾朋子、長尾達也、金丸周司、有坂文雄
  • Organizer: 第7回日本蛋白質科学会年会.
  • Place of Presentation: 仙台国際センター
• [Presentation] T4ファージ基盤終結蛋白質gp48、gp54の大量発現系の構築と性状解析 (2007)
  • Author(s): 伊藤麻紗子、金丸周司、有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ
  • Place of Presentation: 熱海
• [Presentation] Crystallization of the C-terminal-truncated mutant proteins of gp34, the proximal half tail fiber of bacteriophage T4 (2007)
  • Author(s): Donny Sunanda、金丸周司、有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ.
  • Place of Presentation: 熱海
• [Presentation] T4類縁ファージのgp5ホモログ蛋白質の性状解析と結晶化 (2007)
  • Author(s): 根本舞、油井孔兵、金丸周司、有坂文雄
  • Organizer: 第3回「生体超分子構造」ワークショップ.
  • Place of Presentation: 熱海
• [Presentation] T4ファージ基盤構成蛋白質gp7,gp10,gp11の相互作用部位の同定 (2007)
  • Author(s): 中尾朋子、長尾達也、金丸周司、有坂文雄
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] T4ファージ基盤終結蛋白質gp48,gp54の大量発現系の構築と性状解析 (2007)
  • Author(s): 伊藤麻紗子、金丸周司、有坂文雄
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Foldon fusion to the C-terminal-truncated mutant proteins of gp34, the proximal half tail fiber of bacteriophage T4 to facilitate trimer formation. (2007)
  • Author(s): Donny Sunanda、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第4回公開シンポジウム
  • Place of Presentation: 大阪
• [Presentation] T4ファージ尾部基盤構成蛋白質gp10・gp11の結合部位の同定 (2006)
  • Author(s): 中尾朋子、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第3回公開シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージgp7のプロテアーゼ抵抗性断片の同定と精製 (2006)
  • Author(s): 長尾達也、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第3回公開シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージ尾鞘蛋白質のN末端欠損変異体の解析 (2006)
  • Author(s): 須貝伸治、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第3回公開シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 人工三本鎖βヘリックスタンパク質の作製 (2006)
  • Author(s): 寺内允、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第3回公開シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] :T4ファージ基盤構成蛋白質gp48の発現、精製と性状解析 (2006)
  • Author(s): 伊藤麻紗子、金丸周司、有坂文雄
  • Organizer: 特定領域研究「生体超分子構造」第3回公開シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Identification of the interaction site of gp10-gp11 complex of Bacteriophage T4. (2006)
  • Author(s): Tomoko, Nakao,Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: Grant-in-Aid for Scientific Research in Priority Areas. The third symposium on "Biological Ssuprarmolecular Struc ture"
  • Place of Presentation: Tukuba
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Identification of the protease-resistant fragment of bacteriophage T4 and its purification. (2006)
  • Author(s): Tatsuya, nagao, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: Grant-in-Aid for Scientific Research in Priority Areas. The third symposium on "Biological Ssuprarmolecular Struc ture"
  • Place of Presentation: Tukuba
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of the N-terminal deletion mutant of Bacteriophage T4. (2006)
  • Author(s): Sinji, Sugai, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: Grant-in-Aid for Scientific Research on Priority Areas. The third open symposium on "Biological Ssuprarmolecular Struc ture"
  • Place of Presentation: Tukuba
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction of the artificial triple-stranded b-helix. (2006)
  • Author(s): Makoto, Terauchi, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: Grant-in-Aid for Scientific Research in Priority Areas. The third symposium on "Biological Ssuprarmolecular Struc ture"
  • Place of Presentation: Tukuba
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Expression, purification and characterization of gp48, a baseplate protein of bacteriophage T4. (2006)
  • Author(s): Masako, Ito, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: Grant-in-Aid for Scientific Research in Priority Areas. The third symposium on "Biological Ssuprarmolecular Struc ture"
  • Place of Presentation: Tukuba
  • Year and Date: 2006-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Oxygen Binding Properties and Dimer-Tetramer Equilibrium of bW37 Mutants of Human Hemoglobin A. (2006)
  • Author(s): Masako Nagai, Yayoi Aki-Jin, Kiyohiro Imai, Fumio Arisaka, Yukifumi Nagai
  • Organizer: Fifth East Asian Biophysics Symposium and 44th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Interactions between Vibrio phage KVP40 gp5 and gene product of ORF334, T4 gp27 homologue. (2006)
  • Author(s): Mai Nemoto, Shuji Kanamaru, Fumio Arisaka
  • Organizer: Fifth East Asian Biophysics Symposium and 44th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Flexible and rigid regions in the stalk domain of ncd motor protein. Fifth East Asian Biophysics Symposium and 44th Annual Meeting of the Biophysical Society of Japan, November 12-16, Naha, Okinawa(2006) (2006)
  • Author(s): Tsukasa Makino, Hiisayuki Morii, Takashi Shimizu, Fumio Arisaka, Yusuke Kato, Koji Nagata, Masaru Tanokura
  • Organizer: Fifth East Asian Biophysics Symposium and 44th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Artificial three-stranded β-helix protein as a nanotube (2006)
  • Author(s): Makoto Terauchi, Shuji Kanamaru, Fumio Arisaka
  • Organizer: Fifth East Asian Biophysics Symposium and 44th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Yayoi Aki-Jin, Kiyohiro Imai, Fumio Arisaka, Yukifumi Nagai : Oxygen Binding Properties and Dimer-Tetramer Equilibrium of βW37 Mutants of Human Hemoglobin A. (2006)
  • Author(s): Masako, Nagai
  • Organizer: Fifth East Asian Biophys. Symposium and 44th Ann. Meeting of the Biophys. Soc. of Japan.
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Interactions between Vibrio phage KVP40 gp5 and gene product of ORF334, T4 gp27 homologue. (2006)
  • Author(s): Mai, Nemoto, Shuji, Kanamaru, Fumio, Arisaka
  • Organizer: Fifth East Asian Biophysics Symposium and 44th Annual Meeting of the Biophys. Soc. of Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Hiisayuki Morii, Takashi Shimizu, Fumio Arisaka, Yusuke Kato, Koji Nagata, Masaru Tanokura Flexible and rigid regions in the stalk domain of ncd motor protein. (2006)
  • Author(s): Tsukasa, Makino
  • Organizer: Fifth East Asian Biophys. Symp. &44th Ann. Meeting of the Biophys. Soc. Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Shuji Kanamaru, Fumio Arisaka : Artificial three-stranded B-helix protein as a nanotube. (2006)
  • Author(s): Makoto, Terauchi
  • Organizer: Fifth East Asian Biophys. Symp. & 44th Annual Meeting of the Biophys. Soc. of Japan
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2006-11-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] バクテリオファージ研究の最近の動向 (2006)
  • Author(s): 有坂文雄
  • Organizer: 大阪大学蛋白質研究所セミナー
  • Place of Presentation: 大阪
  • Year and Date: 2006-09-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージの構造生物学 (2006)
  • Author(s): 金丸周司
  • Organizer: 大阪大学蛋白質研究所セミナー
  • Place of Presentation: 大阪
  • Year and Date: 2006-09-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Recent Research Progress on Bacteriophage (2006)
  • Author(s): Fumio, Arisaka
  • Organizer: Protein Institute Seminar, Institute for Protein Research, Osaka University
  • Place of Presentation: Osaka
  • Year and Date: 2006-09-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structural biology of Bacteriophage T4 (2006)
  • Author(s): Shuji, Kanamaru
  • Organizer: Protein Institute Seminar, Institute for Protein Research, Osaka University
  • Place of Presentation: Osaka
  • Year and Date: 2006-09-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] BIACore K. K: Protein Interactions in Biology(organizer) (2006)
  • Author(s): Fumio Arisaka
  • Organizer: 20th IUBMB International Congress of Biochem istry and Molecular Biology and 11th FAOBM Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] BIACore K. K Importance of Quantitative Measurement of Protein Irateractions in Post-Genome Sequencing Era (2006)
  • Author(s): Fumio Arisaka
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] How Glycosylation Activates Multifunctional Extracellular MatrixGlycoprotein, Vitronectin, during Liver Regeneration (2006)
  • Author(s): Kotone Sano, Kimie Asanuma, Nana kawasaki, Fumio Arisaka, Haruko Ogawa
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Incorporation of unnatural amino acid by in vitro translation systemfor detection of the conformation change of confactorbinding protein (2006)
  • Author(s): Masato Abe, Takashi Yokogawa, Satoshi Ohno, Masaaki Suzuki, Takamitsu Hosoya, Takeshi Nakanishi, FumioArisaka, Tomio Ogasawara, Tatsuya Sawasaki, Kazaya Nishikawa, Masaya Kitamura, Niroyuki Hori, Yaeta Endo
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] BIACoreK.K : Protein Interactions in Biology (organizer) (2006)
  • Author(s): Fumio, Arisaka
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] BIACore K.K Importance of Quantitative Measurement of Protein Interactions in Post-Genome Sequencing Era. (2006)
  • Author(s): Fumio, Arisaka
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Kimie Asanuma, Nana kawasaki, Fumio Arisaka, Haruko Ogawa : How Glycosylation Activates Multifunctional Extracellular Matrix Glycoprotein, Vitronectin, during Liver Regeneration. (2006)
  • Author(s): Kotone, Sano
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Takashi Yokogawa, Satoshi Ohno, Masaaki Suzuki, Takamitsu Hosoya, Takeshi Nakanishi, FumioArisaka,Tomio Ogasawara, Tatsuya Sawasaki,Kazaya Nishikawa,Masaya Kitamura, Hiroyuki Hori, Yaeta Endo : Incorporation of unnatural amino acid by in vitro translation system for detection of the conformation change of confactorbinding protein. (2006)
  • Author(s): Masato, Abe
  • Organizer: 20th IUBMB International Congress of Biochem. & Mol. Biol. and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The gene product of ORF334 of Vibrio phage KVP40 is the homologue of T4 gp27 which binds to the tail lysozyme, gp5 (2006)
  • Author(s): Mai Nemoto, Suji Kanamaru, Fumio Arisaka
  • Organizer: 2006 FASEB Summer Research Conferences, Virus Assembly
  • Place of Presentation: Vermont Academy, Saxtons River, Vermont
  • Year and Date: 2006-06-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification, characterization and solution interactions of the neck proteins, gp13 and gp14, from Bacteriophage T4. (2006)
  • Author(s): Tahmina Akhter, Shuji Kanamaru and Fumio Arisaka
  • Organizer: 2006 FASEB Summer Research Conferences, Virus Assembly
  • Place of Presentation: Vermont Academy, Saxtons River, Vermont
  • Year and Date: 2006-06-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The gene product of ORF334 of Vibrio phage KVP40 is the homologue of T4 gp27 which binds to the tail lysozyme, gp5. (2006)
  • Author(s): Mai, Nemoto, Suji, Kanamaru, Fumio, Arisaka
  • Organizer: 2006 FASEB Summer Res.Conf., Virus Assembly, Vermont Academy, Saxtons River
  • Place of Presentation: Vermont
  • Year and Date: 2006-06-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Shuji Kanamaru and Fumio Arisaka : Purification, characterization and solution interactions of the neck proteins, gp13 and gp14, from Bacteriophage T4. (2006)
  • Author(s): Tahmina, Akhter
  • Organizer: 2006 FASEB Summer Res.Conf., Virus Assembly, Vermont Academy, Saxtons River
  • Place of Presentation: Vermont
  • Year and Date: 2006-06-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structure and Function of the Tail-lysozyme of T4-related Bacteriophages. (2006)
  • Author(s): Mai Nemoto, Shuji Kanamaru, Fumio Arisaka
  • Organizer: The HFSP 17th Anniversary-Sixth Awardees Annual Meeting
  • Place of Presentation: Paris
  • Year and Date: 2006-06-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Shuji Kanamaru, Fumio Arisaka Structure and Function of the Tail-lysozyme of T4-related Bacteriophages. (2006)
  • Author(s): Mai, NemotoL
  • Organizer: The HFSP 17th Anniversary-Sixth Awardees Ann. Meeting
  • Place of Presentation: Paris
  • Year and Date: 2006-06-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 相互作用測定法外観と超遠心分析に基づく蛋白質間相互解析. (2006)
  • Author(s): 有坂文雄
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 京都
  • Year and Date: 2006-04-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ビブリオファージKVP40におけるT4gp27ホモログの同定とテイルルゾチームgp5との結合. (2006)
  • Author(s): 根本舞、金丸周司、有坂文雄:
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 京都
  • Year and Date: 2006-04-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] T4ファージ尾鞘蛋白質の重合化に関する変異体の解析. (2006)
  • Author(s): 須貝伸治、金丸周司、有坂文雄
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 京都
  • Year and Date: 2006-04-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Overview of methodology of measurements and analysis of protein- protein interactions by analytical ultracentrifugation. (2006)
  • Author(s): Fumio, Arisaka
  • Organizer: The 6th Ann. Meeting of the Prot. Sci. Soc. Japan
  • Place of Presentation: Kyoto
  • Year and Date: 2006-04-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fumio Arisaka The gene product of ORF334 of Vivrio phage KVP40 is the homologue of T4 gp27 which binds to the tail lysozyme,gp5. (2006)
  • Author(s): Mai, Nemoto, Suji, Kanamaru
  • Organizer: The 6th Ann. Meeting of the Prot.Sci.Soc. Japan
  • Place of Presentation: Kyoto
  • Year and Date: 2006-04-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fumio Arisaka : Analysis of mutant sheath protein of phage T4 in relation to Polymerization. (2006)
  • Author(s): Sinji, Sugai, Suji, Kanamaru
  • Organizer: The 6th Ann. Meeting of the Prot.Sci.Soc. of Japan
  • Place of Presentation: Kyoto
  • Year and Date: 2006-04-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 東京工業大学大学院生命理工学研究科編「トコトンやさしいタンパク質の本」 (2007)
  • Author(s): 有坂文雄
  • Total Pages: 12
  • Publisher: 日刊工業新聞社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 超遠心分析による蛋白質間相互作用の解析、「分子間相互作用解析ハンドブック」礒辺・中山・伊藤編 (2007)
  • Author(s): 有坂文雄
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 日刊工業新聞社 (2007)
  • Author(s): 有坂文雄
  • Total Pages: 12
  • Publisher: 東京工業大学大学院生命理工学研究科編「トコトンやさしいタンパク質の本」