| Budget Amount *help |
¥3,840,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Research Abstract |
Stretch activated and Ca (2+) activated big K channel (SAKcaC) identified by our group, is one form of splice variants of the Moe channel encoded by SIo-1 gene, and has the STREX-sequence, a 59 amino-add splice insert located in the cytoplasmic side of the channel, which may responsible fir the membrane stretch sensitivity of SAKcaCs. Recently, we found that most SAKcaCs exhibited simple diffusion on the plasma membrane but that approximatly 25% oldie channels was immobilized on or near focal contacts (FCs), suggesting that SAKcaCs are associated with the FC components such as adhesion molecules and cytoskeletons to form a mechano-sensing device. However the mechanisms of such channel immobilization on FC remains unclear Tb address this issue, we examined the role of STREX on the channel immobilizatin We expressed GFP-tagged STREX and observed using the single fluorophore video imaging technique. Simultaneous observation with RFP-tagged paxillin showed that STREX molecules were recruited and diffused rapidly on the plasma membrane but significant fraction of them was immobilized on FCs, indicating that STREX itself could associate with certain FC components. However, STREX-deleted SAKcaCs were also immobilized on or near FCs, indicating that SAKcaCs could be associated with Its via STEER and/or other sites of the channel
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