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A study of functional RNA biogenesis

Research Project

Project/Area Number 18570157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionHirosaki University

Principal Investigator

USHIDA Chisato  Hirosaki University, 農学生命科学部, 准教授 (50250593)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥690,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords遺伝情報複製 / 転写装置 / 再編 / 制御 / non-coding RNA / ncRNA / 転写 / 線虫 / PSE / ncRNA遺伝子 / プロモーター / イントロン
Research Abstract

多細胞生物における内在性ncRNAの合成や局在を決定する分子メカニズムの解明、制御機構の理解を目指し、またこれらについての新たなシステムの発見を期待して、線虫(Caenorhabditis elegans)の新規ncRNAの転写や局在を解析した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (28 results)

All 2009 2008 2007 2006 2005 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (17 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Distribution of U3 small nucleolar RNA and fibrillarin during early embryogenesis in Caenorhabditis elegans2008

    • Author(s)
      Sasano, Y., Hokii, Y., Inoue, K., Sakamoto, H., Ushida, C., Fujiwara, T.
    • Journal Title

      Biochimie 90

      Pages: 898-907

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of U3 small nucleolar RNA and fibrillarin during early embryogenesis in Caenorhabditis elegans2008

    • Author(s)
      Sasano, Y., Hokii, Y., Inoue, K Sakamoto, H., Ushida, C., Fujiwara, T.
    • Journal Title

      Biochimie 90

      Pages: 898-907

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of U3 small nucleolar RNA and fibrillarin during early embryogenesis in Caenorhabditis elegans2008

    • Author(s)
      Sasano, Y., Hokii, Y., Inoue, K., Sakamoto, H., Ushida, C., *Fujiwara, T.
    • Journal Title

      Biochimie (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Twelve novel C. elegans RNA candidates isolated by two-dimensional polyacrylamide gel electrophoresis2005

    • Author(s)
      Yusuke Hokii, Akiyoshi Kubo, Takahiro Ogasawara, Yuhkou Nogi, Akito Taneda, Risa Arai, Akira Muto, Chisato Ushida.
    • Journal Title

      Gene 365

      Pages: 83-87

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] 線虫pre-rRNAプロセッシングに働く線虫新規ncRNA関連タンパク質の解析2009

    • Author(s)
      保木井悠介, 笹野有未, 武藤呈, 藤原俊伸, 牛田千里
    • Organizer
      第3回無細胞生命科学研究会
    • Place of Presentation
      弘前大学創立50周年記念会館
    • Year and Date
      2009-03-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] C. elegansにおけるsnoRNA結合タンパク質のノックダウンと核内構造体の形成2008

    • Author(s)
      保木井悠介、菅原由起、笹野有未、藤原俊伸、武藤呈、牛田千里
    • Organizer
      RNAフロンティアミーティング2008
    • Place of Presentation
      関西セミナーハウス(京都市)
    • Year and Date
      2008-06-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Expression and localization of C. elegans CeR-5 RNA2008

    • Author(s)
      Chisato Ushida, Yuki Sugawara and Yusuke Hokii
    • Organizer
      Keystone symposia RNAi, microRNA, and non-coding RNA
    • Place of Presentation
      Whistler Resort, Whistler
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 核小体局在を示す線虫新規ncRNA2008

    • Author(s)
      保木井悠介、佐藤洋旭、武藤〓、牛田千里
    • Organizer
      第10回日本RNA学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Expression and localization of C2008

    • Author(s)
      Chisato Ushida, Yuki Sugawara and Yusuke Hokii
    • Organizer
      elegans CeR-5 RNA. Keystone symposium, RNAi, Micro RNA, and Non-coding RNA
    • Place of Presentation
      Whistler Resort, Whistler, British Columbia, Canada
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫新規低分子ncRNAの局在解析2007

    • Author(s)
      牛田千里、保木井悠介、菅原由起、遠藤優子、佐藤洋旭、武藤 〓
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会)
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] 線虫snoRNPタンパク質遺伝子ノックダウン株におけるsnoRNAの局在変化2007

    • Author(s)
      菅原由起、保木井悠介、武藤 〓、牛田千里
    • Organizer
      第9回 日本RNA学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] 受精前後の卵における線虫Caenorhabditis elegans核小体低分子RNAの動態2007

    • Author(s)
      保木井悠介、笹野有未、藤原俊伸、坂本 博、武藤〓 、牛田千里
    • Organizer
      第9回 日本RNA学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] 線虫の機能RNomics2007

    • Author(s)
      牛田千里、小笠原隆広、天川純一、遠藤優子、菅原由起、武藤 〓、保木井悠介
    • Organizer
      第40回日本発生生物学会 第59回日本細胞生物学会 合同大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 線虫新規低分子ncRNAの局在解析2007

    • Author(s)
      牛田千里、保木井悠介、菅原由起、遠藤優子、佐藤洋旭、武藤〓
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫snoRNPタンパク質遺伝子ノックダウン株におけるsnoRNAの局在変化2007

    • Author(s)
      菅原由起、保木井悠介、武藤 〓、牛田千里
    • Organizer
      第9回日本RNA学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2008 Final Research Report
  • [Presentation] 受精前後の卵における線虫Caenorhabditis elegans核小体低分子RNAの動態2007

    • Author(s)
      保木井悠介、笹野有未、藤原俊伸、坂本博、武藤 〓、牛田千里
    • Organizer
      第9回日本RNA学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2008 Final Research Report
  • [Presentation] 線虫の機能Rnomics2007

    • Author(s)
      牛田千里、小笠原隆広、天川純一、遠藤優子、菅原由起、武藤〓、保木井悠介
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2008 Final Research Report
  • [Presentation] Spatio temporal distribution patterns of C. elegans small ncRNAs2006

    • Author(s)
      Hokii, Y., Sugawara, Y., Endo, Y., Mizuki, Y., Harada, F., Konno, T., Himeno, H., Muto, A., Ushida, C.
    • Organizer
      East Asia C. elegans Meeting
    • Place of Presentation
      Hoam Convention Center, Seoul National University, Seoul, Korea.
    • Related Report
      2008 Final Research Report
  • [Presentation] C.elegans 低分子RNACeR-2RNAの細胞内局在と生合成2006

    • Author(s)
      保木井悠介, 笹野由未, 藤原俊伸, 坂本博, 種田晃人, 武藤〓, 牛田千里
    • Organizer
      日本RNA学会
    • Place of Presentation
      淡路夢舞台国際会議場
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of Cen21/CeR-2 RNA, small ncRNA localized in Caenorhabditis elegans nucleoli2006

    • Author(s)
      Yusuke Hokii, Masayoshi Shimoyama, Akira Muto, Chisato Ushida.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto, Japan.
    • Related Report
      2008 Final Research Report
  • [Presentation] Expression of C. elegans novel ncRNAs2006

    • Author(s)
      Chisato Ushida, Yusuke Hokii, Yuki Sugawara, Yuko Mizuki, Yuko Endo, Akira Muto
    • Organizer
      Regularoty RNAs,the LXXI Cold Spring Harbor Symposium
    • Place of Presentation
      Cold Spring Harbor, New York, U. S. A
    • Related Report
      2008 Final Research Report
  • [Book] Isolation and characterization of small RNAs in Caenorhabditis elegans John Rossi and Rajesh K. Gaur eds. Regulation of Gene Expression by Small RNAs2009

    • Author(s)
      Chisato Ushida, Yusuke Hokii
    • Publisher
      CRC Press, Florida, U. S. A. Chapter 7
    • Related Report
      2008 Final Research Report
  • [Book] Isolation and characterization of small RNAs in Caenorhabditis elegans, in Regulation of gene expression by small RNAs. Gaur, R. K. and Rossi, J. J. eds2009

    • Author(s)
      Chisato Ushida and Yusuke Hokii
    • Publisher
      CRC Press, Florida, U.S.A.
    • Related Report
      2008 Annual Research Report
  • [Book] 線虫にみるRNA機能の多様性、河合剛太、金井昭夫(編集)、機能性Non-coding RNA、第1部第2章2006

    • Author(s)
      牛田千里
    • Publisher
      クバプロ
    • Related Report
      2008 Final Research Report
  • [Book] 機能性Non-coding RNA(河合剛太、金井昭夫編)、線虫に見るRNA機能の多様性2006

    • Author(s)
      牛田 千里
    • Total Pages
      255
    • Publisher
      クバプロ
    • Related Report
      2006 Annual Research Report
  • [Remarks]

    • URL

      http://nature.cc.hirosaki-u.ac.jp/kohou2/public/oyo/c.ushida/index.htm

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://rna.eit.hirosaki-u.ac.jp/rrc/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://nature.cc.hirosaki-u.ac.jp/kohou2/public/oyo/c.ushida/index.htm

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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