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Roles of chromatin structure in regulation of gene activity

Research Project

Project/Area Number 18570158
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTohoku University

Principal Investigator

KOMURA Jun-ichiro  Tohoku University, Tohoku University, Graduate School of Medicine, Assistant Professor (10215410)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordschromatin structure / gene activity / 遺伝子 / 発現制御 / 遺伝子発現
Research Abstract

During mitosis in higher eukaryotic cells, transcription is silenced and transcription complexes are absent from promoters in the condensed chromosomes; however, epigenetic information concerning the pattern of expressed and silent genes must be preserved. Recently it has been reported that CTCF, a major protein in vertebrate insulator elements, then it is possible that epigenetic information through the mitotic phase, and help guide the reconstitution of domain structure and nuclear organization after the completion of this phase. We have studied the chromatin structure of the insulator upstream of the c-MYC gene in mitotic HeLa cells. The region of the insulator corresponds to the DNase I hypersensitive site I, but Southern analysis revealed that hypersensitivity was lost during motosis. High resolution in vivo footprinting analysis using dimethyl sulfate, UV, psoralen, and DNase I also demonstrated the disappearance of the sequence-specific direct binding of CTCF and the absence of detectable structures during mitosis. Thus, it appears that the nucleoprotein complex involving this insulator element must be reassembled de novo with each new cell generation.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (7 results)

All 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 4 results)

  • [Journal Article] Chromatin fine structure of the c-MYC insulator element/DNase I-hypersensitive site I is not preserved during mitosis.2007

    • Author(s)
      Jun-ichiro Komura
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 15741-15746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional interactions between BLM and XRCC3 in the cell.2007

    • Author(s)
      Makoto Otsuki
    • Journal Title

      JCB 179

      Pages: 53-63

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Chromatin fine structure of the c-MYC insulator element/DNase l-hypersensitivesite I is not preserved during mitosis2007

    • Author(s)
      Jun-ichiro Komura
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 15741-15746

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional interactions between BLM and XRCC3 in the cell2007

    • Author(s)
      Makoto Otsuki
    • Journal Title

      JCB 179

      Pages: 53-63

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chromatin fine structure of the c-MYC insulator element/DNase I-hypersensitive site I is not preserved during mitosis.2007

    • Author(s)
      Jun-ichiro Komura
    • Journal Title

      Proceedings of the National Academy of Sciences 104

      Pages: 15741-15746

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional interactions between BLM and XRCC3 in the cell.2007

    • Author(s)
      Makoto Otsuki
    • Journal Title

      The Journal of Cell Biology 179

      Pages: 53-63

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The maintenance of genome integrity is tissue-specifie.2006

    • Author(s)
      Tetsuya Ono
    • Journal Title

      Genes and Environment 28・1

      Pages: 16-22

    • Related Report
      2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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