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Analysis of LRK-1 kinase which regulates polarized sorting in neurons

Research Project

Project/Area Number 18570174
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionNagoya University

Principal Investigator

HISAMOTO Naoki  Nagoya University, Biological Science, Associate professor (80283456)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsPolarized transport / kinase / neuron / C.elegans / C.elegans / C. elegans
Research Abstract

Neurons are polarized cells that contain distinct sets of proteins in their axons and dendrites. Synaptic vesicles(SV)and many SV proteins are exclusively localized in the presynaptic regions but not in dendrites. Despite their fundamental importance, the mechanisms underlying the polarized localization of SV proteins remain unclear. The transparent nematode Caenorhabditis elegans can be used to examine sorting and transport of SV proteins in vivo. Here, we identify a novel protein kinase LRK-1, a C. elegans homolog of the familial Parkinsonism gene PARK8/LRRK2 that is required for polarized localization of SV proteins. In Irk-1 deletion mutants, SV proteins are localized to both presynaptic and dendritic endings in neurons. This aberrant localization of SV proteins in the dendrites is dependent on the AP-1 ml clathrin adaptor UNC-101, which is involved in polarized dendritic transport, but not on UNC-I 04 kinesin, which is required for the transport of SV to presynaptic regions. The LRK-1 proteins are localized in the Golgi apparatus. These results suggest that the LRK-1 protein kinase determines polarized sorting of SV proteins to the axons by excluding SV proteins from the dendrite-specific transport machinery in the Golgi.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] C. elegans WNK-STE20 pathway regulates tube formation by mod ulating ClC channel activity.2008

    • Author(s)
      Hisamoto N, et. al.,
    • Journal Title

      EMBO reports 9

      Pages: 70-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] C. elegans WNK-STE20 pathway regulates tube formation by modulating CIC channel activity2008

    • Author(s)
      Hisamoto, N, et. al.
    • Journal Title

      EMBO reports 9

      Pages: 70-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] C. elegans WNK-STE20 pathway regulates tube formation by modulating C1C channel activity.2008

    • Author(s)
      Hisamoto N, et. al.
    • Journal Title

      EMBO reports 9

      Pages: 70-75

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LRK-1, a C. elegans PARK8-related kinase, regulates axonal-dendritic polarity of SV proteins2007

    • Author(s)
      Sakaguchi-Nakashima, et. al.
    • Journal Title

      Current Biology 17

      Pages: 592-598

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] LRK-1, a C.elegans PARK8-related kinase, regulates axonal-dendritic polarity of SV proteins2007

    • Author(s)
      Sakaguchi, -Nakashima, et. al.
    • Journal Title

      Current Biology 17

      Pages: 592-598

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] LRK-1, a C.elegans PARK8-related kinase, regulates axonal-dendritic polarity of SV proteins2007

    • Author(s)
      Sakaguchi-Nakashima et al.
    • Journal Title

      Current Biology 17

      Pages: 592-598

    • Related Report
      2006 Annual Research Report
  • [Presentation] 種を越えて保存されたBAG-2およびCHIPによるLRRKの制御機構2007

    • Author(s)
      福島沖之, ら
    • Organizer
      日本生化学会・日本分子生物学会合同年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] A regulatory mechanism of LRRK by BAG-2 and CHIP is conserved beyond species2007

    • Author(s)
      Fulcusima, et. al.
    • Organizer
      BMMB2007
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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