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Regulation mechanism of adipocyte differentiation through prostaglandin D2 receptor

Research Project

Project/Area Number 18570187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionOsaka University of Pharmaceutical Sciences (2007)
Osaka Bioscience Institute (2006)

Principal Investigator

FUJIMORI Ko  Osaka University of Pharmaceutical Sciences, 薬学部, Lecturer (70425453)

Co-Investigator(Kenkyū-buntansha) URADE Yoshihiro  Osaka Bioscience Institute, 分子行動生物学部門, Department Head (10201360)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsprostaglandin D2 / adipocyte / regulation of differentiation / regulation of obesity / molecular biology / transcriptional regulation / 転写調飾 / 分化 / 受容体 / 核内受容体
Research Abstract

Lipocalin-type prostaglandin (PG) D synthase (L-PGDS) is expressed in adipocytes and is proposed to be involved in the regulation of glucose tolerance and atherosclerosis in type 2 diabetes, because L-PGDS gene knock-out mice show abnormalities in these functions. However, the role of L-PGDS and the regulation mechanism governing its gene expression in adipocytes remain unclear. I applied small interference RNA of L-PGDS to mouse 3T3-L1 cells and found that it suppressed differentiation of these cells into adipocytes. Reporter analysis of the mouse L-PGDS promoter demonstrated that a responsive element for liver receptor homolog-1 (LRH-1) at -233 plays a critical role in preadipocytic 3T3-L1 cells. Moreover, we identified two sterol regulatory elements (SREs) at -194 to be ciselements for activation of L-PGDS gene expression in adipocytic 3T3-L1 cells. L-PGDS mRNA was induced in response to synthetic liver X receptor agonist, T0901317, through activation of the expression of SRE-binding protein-1c (SREBP-1c) in the adipocytic 3T3-L1 cells. The results of electrophoretic mobility shift assay and chromatin immunoprecipitation assay revealed that LRH-1 and SREBP-1c bound to their respective binding elements in the promoter of L-PGDS gene. Small interference RNA-mediated suppression of LRH-1 or SREBP-1c decreased L-PGDS gene expression in preadipocytic or adipocytic 3T3-L1 cells, respectively. These results indicate that L-PGDS gene expression is activated by LRH-1 in preadipocytes and by SREBP-1c in adipocytes. Liver X receptor-mediated up-regulation of L-PGDS through activation of SREBP-1c is a novel pathway to enhance adipocyte differentiation.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (14 results)

All 2007

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (3 results) Book (2 results)

  • [Journal Article] A novel pathway to enhance adipocyte differentiation of 3T3-L1 cells by up-regulation of lipocalin-type prostaglandin D synthase mediated by liver X receptor-activated sterol regulatory element bindingprotein-1c2007

    • Author(s)
      Fujimori, K.
    • Journal Title

      J.Biol.Chem. 282

      Pages: 18458-18466

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] NMR solution structure of lipocalin-type prostaglandin D synthase:Evidence for partial overlapping of catalytic pocket and retinoic acid-binding pocket within the central cavity2007

    • Author(s)
      Shimamoto, S.
    • Journal Title

      J.Biol.Chem. 282

      Pages: 31373-31379

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 肥満防止薬に道!脂肪を蓄積させる酵素を確認2007

    • Author(s)
      藤森 功
    • Journal Title

      化学 62

      Pages: 73-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] A novel pathway to enhance adipocyte ifferentiation of3T3-Ll cells by up-regulation of lipocalin-type prostaglandin D synthase mediated by liver X receptor-activated sterol regulatory element bindingprotein- Ic2007

    • Author(s)
      Fujimori, K.
    • Journal Title

      J. Biol. Chem. 282

      Pages: 18458-18466

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] NMR solution structure of lipocalin-type prostaglandin D synthase : Evidence for partial overlapping of catalytic pocket and retinoic acid-binding pocket within the central cavity2007

    • Author(s)
      Shimamoto, S.
    • Journal Title

      J. Biol. Chem. 282

      Pages: 31373-31379

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Confirmation of the enzyme involved in the accumulation of lipids2007

    • Author(s)
      Fujimori, K.
    • Journal Title

      Kagaku 62

      Pages: 73-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prostaglandin D_2 Synthase - A Multitude of Biological Functions2007

    • Author(s)
      Fuj imori, K.
    • Pages
      1-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A novel pathway to enhance adipocyte differentiation of 3T3-L1 cells by up-regulation of lipocalin-type prostaglandin D synthase mediated by liver X receptor-activated sterol regulatoy element bindingprotein-1c2007

    • Author(s)
      Fujimori, K.
    • Journal Title

      J.Biol.Chem 282

      Pages: 18458-18466

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMR solution structure of lipocalin-type prostaglandin D synthase: Evidence for partial overlapping of catalytic pocket and retinoic acid-binding Pocket within the central cavity2007

    • Author(s)
      Shimamoto, S.
    • Journal Title

      J.Biol.Chem 282

      Pages: 31373-31379

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] プロスタグランジン類による脂肪細胞の分化調節2007

    • Author(s)
      藤森 功
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会 合同大会
    • Place of Presentation
      パシフィコ横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of adipocyte differentiation by prostaglandins2007

    • Author(s)
      Fujimori, K.
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] プロスタグランジン類による脂肪細胞の分化調節2007

    • Author(s)
      藤森 功
    • Organizer
      第30回N本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Book] Prostaglandin D_2 Synthase-A Multitude of Biological Functions2007

    • Publisher
      Research Signpost
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Prostaglandin D2 synthase-A multitude of biological functions2007

    • Author(s)
      Fujimori, K.
    • Publisher
      Research Signpost
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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