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Functional analysis of cnil ; an intra-cellular transporter of EGF-repeat-containing growth factors

Research Project

Project/Area Number 18570195
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

CHISAKA Osamu  Kyoto University, Graduate Schcol of Biostudies, Associate Professor (80188474)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordscornichon-like protein / cornichon homolog 2 / neural crest cells / cranial nerve / HB-EGF / ErbB4 / secretion / migration / 細胞移動
Research Abstract

During their migration to the periphery, cranial neural crest cells (NCCs) are repulsed by an ErbB4-dependent cue(s) in the mesenchyme adjoining rhombomeres (r) 3 and 5, which are segmented hindbrain neuromeres. ErbB4 has many ligands, but which ligand functions in the above system has not yet been clearly determined. Here we found that a cornichon-like protein/cornichon homolog 2 (CNIL/CNIH2) gene was expressed in the developing chick r3 and r5. In a cell culture system, its product facilitated the secretion of heparin-binding epidermal growth factor-like growth factor (HB-EGF), one of the ligands of ErbB4. When CNIL function was perturbed in chick embryos by forced expression of a truncated form of CNIL, the distribution of NCCs was affected, which resulted in abnormal nerve fiber connections among the cranial, sensory ganglia. Also, knockdown of CNIL or HB-EGF with siRNAs yielded a similar phenotype. This phenotype closely resembled that of ErbB4 knockout (KO) mouse embryos. Since HB-EGF was uniformly expressed in the embryonic hindbrain, CNIL seems to confine the site of HB-EGF action to r3 and r5 in concert with ErbB4.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (3 results)

All 2007

All Journal Article (3 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Cornichon-like Protein Facilitates Secretion of HB・EGF and Regulates Proper Development of Cranial Nerves2007

    • Author(s)
      Hoshino, H., et. al.
    • Journal Title

      Molecular Biology of the Cell 18

      Pages: 1143-1152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cornichon-like Protein Facilitates Secretion of HB-EGF and Regulates Proper Development of Cranial Nerves2007

    • Author(s)
      Hideharu, Hoshino, Tsukasa, Uchida, Toshiaki. Otsuki, Shoko. Kawamoto, Kousaku, Okubo, Masatoshi, Takeichi, Osamu, Chisaka
    • Journal Title

      Molecular Biology of the Cell Vol.18

      Pages: 1143-1152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cornichon-like Protein Facilitates Secretion of HB-EGF and Regulates Proper Development of Cranial Nerves2007

    • Author(s)
      Hoshino, H., et. al.
    • Journal Title

      Molecular Biology of the Cell 18

      Pages: 1143-1152

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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