Study of early mouse embryogenesis by the observing the cell shape

• Project/Area Number: 18570196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: FUJIMORI Toshihiko Kyoto University, Graduate School of Medicine, Assistant Professor (80301274)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: mouse / blastocyst / cell shape / 細胞分化 / 初期胚 / 細胞 / イメージング

Research Abstract

In mouse, embryonic development proceeds within the oviduct and the uterus. Embryo reaches the stage called the blastocyst three days after the fertilization. In the blastocyst, two types of cells are observed, and this is the first differentiation in mouse development. Cells of Inner cell mass (ICM) contribute to embryo proper and some extra embryonic tissue, whereas cells of trophectoderm (TE) contribute only to extra embryonic ectoderm including the placenta. It is known that Oct4 and Cdx2 are important for the differentiation and maintenance of characters of ICM and TE respectively. We analyzed the expression profiles of these gene products relating to the precise positions of cells within the developing embryo. Few cells were observed that are not facing to the embryonic surface (these cells are defined as inner cells) until the late 8-cell stage. After the next cell division, numbers of inner cells increased along the developmental timing. Cdx2 was first detected at late 8-cell stage, but its expression was very weak. There was no clear relationship between the position of cells and the levels of Cdx2 expression. After the 16-cell stage, the levels of Cdx2 expression increased in outer cells, these are facing to the embryonic surface. And its expression was localized to the TE cells at the blastocyst. On the other hand, expression of Oct4 was evenly distributed up to 32-cell stage. These results suggested that the earliest expression of Cdx2 is not depending on the position of cells but depend on the developmental timing, and the up-regulation of Cdx2 expression was depending on the relative position of cells within the embryo.

Research Products

• [Journal Article] Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape (2007)
  • Author(s): Kurotaki, et. al.
  • Journal Title: Science 316 Pages: 719-723
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] alpha-Klotho as a regulator of calcium homeostasis (2007)
  • Author(s): Imura, et. al.
  • Journal Title: Science 316 Pages: 1645-1618
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor(PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-beta in murine nucleus tractus solitarius (2007)
  • Author(s): Ohi, et. al.,
  • Journal Title: Brain Res 1159 Pages: 77-85
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] マウス胚における初期の軸形成 (2007)
  • Author(s): 藤森俊彦
  • Journal Title: 細胞工学 26 Pages: 1059-1063
  • NAID: 40015626081
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] ライブイメージングによる細胞トレーシングシステムの開発と応用 (2007)
  • Author(s): 藤森俊彦
  • Journal Title: バイオテクノロジージャーナル 7 Pages: 710-712
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape (2007)
  • Author(s): Kurotaki, Y., Hatta, K., Nakao, K., Nabeshima, Y., Fujimori, T.*
  • Journal Title: Science 316 Pages: 719-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] alpha-Klotho as a regulator of calcium homeostasis (2007)
  • Author(s): Imura, A., Tsuji, Y., Murata, M., Maeda R., Kubota, K., Iwano, A., Obuse, C., Togashi, K., Tominaga, M., Kita, N., Tomiyama, K., Iijima, J., Nabeshima, Y., Fujioka, M., Asato, R., Tanaka, S., Kojima, K., Ito, J., Nozaki, K., Hashimoto, N., Ito, T., Nishio, T., Uchiyama, T., Fujimori, T., Nabeshima, Y
  • Journal Title: Science 316 Pages: 1615-8
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Platelet-derived growth factor (PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-beta in murine nucleus tractus solitarius (2007)
  • Author(s): Ohi, Y., Ishii, Y., Haji, A., Noguchi, S., Sasaoka, T., Fujimori, T., Nabeshima, Y.I., Sasahara, M., Hattori, Y
  • Journal Title: Brain Res 1159 Pages: 77-85
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Early axis formation in mouse embryo (2007)
  • Author(s): T., Fujimori
  • Journal Title: Cell technology (Japanese) 26 Pages: 1059-1063
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell tracing system using timelapse live imaging (2007)
  • Author(s): T., Fujimori
  • Journal Title: Biotechnology journal (Japanese) 7 Pages: 710-712
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape (2007)
  • Author(s): Kurotaki Y.
  • Journal Title: Science 316 Pages: 719-723
  • Peer Reviewed
• [Journal Article] Blastocyst axis is specified independently of early cell lineage but aligns with zona pellucida shape
  • Author(s): 黒滝陽子, 八田公平, 中尾和貴, 鍋島陽一, 藤森俊彦
  • Journal Title: Science (in press)
• [Presentation] マウス着床前胚のイメージング法 (2008)
  • Author(s): 藤森俊彦
  • Organizer: 幹細胞の可塑性と未分化性維持機構 成果公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-02-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Liveimaging of the mouse pre-implantation embryo (2008)
  • Author(s): Fujimori, T
  • Organizer: Symposium on Mechanisms underlying self-renewal and pluripotency of the stem cells
  • Place of Presentation: Tokyo
  • Year and Date: 2008-02-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Morphological organization of mouse preimplantation embryo (2007)
  • Author(s): Fujimori, et. al.,
  • Organizer: 2nd SGI International Summit on Reproductive Medicine
  • Place of Presentation: ヴァレンシア、スペイン
  • Year and Date: 2007-11-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Morphological organization of mouse pre-implantation embryo (2007)
  • Author(s): Fujimori, T
  • Organizer: 2nd SGI International Summit on Reproductive Medicine
  • Place of Presentation: Valncia, Spain
  • Year and Date: 2007-11-08
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Morphological organization of mouse preimplantation embryo. (2007)
  • Author(s): Fujimori, T
  • Organizer: 2nd SGI International Summit on Reproductive Medicine
  • Place of Presentation: Valencia, Spain
  • Year and Date: 2007-11-08
• [Presentation] マウス着床前発生を考える (2007)
  • Author(s): 藤森俊彦
  • Organizer: 発生生物学会秋期シンポジウム
  • Place of Presentation: 岡崎
  • Year and Date: 2007-11-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Study of pre-implantation mouse embryo (2007)
  • Author(s): Fujimori, T
  • Organizer: Autumn Symposiun on Developmental biology
  • Place of Presentation: Okazaki
  • Year and Date: 2007-11-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 細胞の振る舞いを観察してマウス着床前胚を考える (2007)
  • Author(s): 藤森俊彦
  • Organizer: モロシヌス研究会
  • Place of Presentation: 淡路
  • Year and Date: 2007-06-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Study of pre-implantation mouse embryo by observing cell behabiors (2007)
  • Author(s): Fujimori, T
  • Organizer: Molosimus research conference
  • Place of Presentation: Awaji
  • Year and Date: 2007-06-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/notice/05_news/documents/070420_1.htm