Function of LIM homeodomain protein Lhx2 in odorant receptor gene regulation
| Project/Area Number |
18570206
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
HIROTA Junji Osaka Prefecture University, Graduate School of Science, Associate Professor (60405339)
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| Co-Investigator(Kenkyū-buntansha) |
ANTHONY Perry RIKEN CDB, 発生再生科学総合センター, Team Leader (80360486)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | olfactory / odorant receptor / gene expression / transgenic / transcription factor / Lhx2 / 神経分化 |
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| Research Abstract |
Odorant receptor (OR) genes can be classified into two types: fish-like class I OR genes and terrestrial-specific class II OR genes. We have previously shown that Lhx2, a LIM-homeodomain protein, binds to the homeodomain site in the promoter region of mouse M71, a class II OR, and that a knockout mutation in Lhx2 precludes expression of all tested class II OR genes including M71. Here, we report that most class I OR genes, which are expressed in a dorsal region of the olfactory epithelium, are still expressed in Lhx2-deficient embryos. There are two exceptions: two class I OR genes, which are normally expressed in a more ventral region, are no longer expressed in Lhx2 mutant mice. Lhx2 is transcribed in olfactory sensory neurons irrespective of expression of class I or class II OR genes. Thus, a deficiency of Lhx2has a differential impact on class I and class II OR gene expression, suggesting that two distinct pathways underlie OR gene expression in mouse.
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Report
(3 results)
Research Products
(18 results)
