Physiological role of fused mRNA of two homeotic genes in crustaceans
| Project/Area Number |
18570208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
SHIGA Yasuhiro Tokyo University of Pharmacy and Life Science, School of Life Sciences, Assistant professor (00277253)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Hox / morphological evolution / translational control / gene regulation / Daphnia |
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| Research Abstract |
To address the physiological roles of the fusion Hox transcripts observed in crustaceans, fused Ubx/Antp transcript in Daphnia magna was analyzed in detail, and the following results were obtained from this research.
1. Various deficient or mutant versions of D.magna Ubx/Antp were constructed and expressed under the control of the heat shock promoter and the actin promoter, however, it was impossible to overcome the problem of mRNAs instabilities from these mutant/deficient constructs, unfortunately.
2. A part of D. magna Hox cluster including Dfd, Hox3, pb, and lab regions was sequenced completely. Also the Hox cluster region in D. pulex, sequenced by an international research organization, Daphnia Genome Consortium, was annotated by comparison with D. magna genomic and cDNA sequences. In Daphnia Hox clusters, two microRNA producing loci, highly conserved among insect genomes, were also conserved. Putative target sequences for these microRNAs were found in 3'-UTR of D. movie Ubx- and Antp. These findings along with above-mentioned observation that fused Ubx/Antp transcripts were, extremely unstable suggested that translational control of HOM proteins from fused transcripts might be mediated by the functions of these microRNAs.
3. RNA interference method in D. magna embryos was developed and some &RNA-mediated morphological defects were confirmed in the corresponding regions with gene expression domains.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Transcriptional readthrough of Hox genes Ubx and Antp and their divergent post-transcriptional control during crustacean evolution2006
Author(s)
Shiga, Y., Sagawa, K., Takai, R., Sakaguchi, H., Yamagata, H., Hayashhi, S.
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Journal Title
Evolution ＆ Development 8・5
Pages: 407-414
Related Report
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[Journal Article] Tissue-specific expression of a bHLH-PAS protein homologous to ARNT during the development of crustacean Daphnia magna2006
Author(s)
Tokishita, S., Kimura, S., Mandokoro, Y., Kato, K., Shiga, Y., Takahashi, Y., Ohta, T., Yamagata, H.
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Journal Title
Related Report
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