| Project/Area Number |
18580013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Crop science/Weed science
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| Research Institution | Kyoto University |
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Principal Investigator |
TANAKA Tomoyuki Kyoto University, Graduate school of agriculture, Associate Professor (50224473)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,750,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Rice / Detached ear culture / Chalky grain |
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| Research Abstract |
This study aimed to elucidate the mechanism about the occurrence of chalky grain rice by establishing and using the long-term detached ear culture method. In the first part of this study the author examined the effect of several anti-oxidants and acidifying reagents addition to culture medium on rice ear growth. Even though the long-term detached ear culture method has not been established, application of either 0.01% sulfurous acid, 5mM L-ascorbate, 1.5mM sulfite or sulfate or sulfite acid seemed to be effective far longer-period ear growth. On the other hand, in order to preliminarily examine hypothesis related to the chalky grain occurrence which has been suggested from research achievement so far using the detached ear culture method, the author conducted field trials on application of gibberellin and gibberellin biosynthesis inhibitors during grain filling period. The results showed that the exogenous gibberellin treatment increased the a-amylase activity of the developing rice seeds and the parentage of the chalky grains even at a rice plant population level (i.e. at a field level), suggesting that both gibberellin and α-amylase are involved in the occurrence of chalky grain. Because the effect of gibberellin biosynthesis inhibitors was not clear in this preliminary study, it will be necessary to precisely examine the influence of concentration, timing, and way of the inhibitor application on the occurrence of chalky grain.
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