Molecular characterization of the RNA-binding protein, TRN-1 in the lepidopteran insects

• Project/Area Number: 18580054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KOTANI Eiji Kyoto Institute of Technology, Graduate Schoolof Seicnce and Technology, Associate Professor (10273541)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥2,540,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: Lepidopteran insect / RNA-binding protein / TIA-1-like protein / Baculovirus / RNA結合蛋白質 / カイコ / タンパク質発現

Research Abstract

TRN-1, an RNA-binding protein homologous with mammalian T-cell intracellular antigen-1 has been found to express in every tissue of the Iepidopteran, Bombyx mori and Spodoptera frugiperda. TRN-1 molecule has been shown to possess the domain structure consisting of three-repetitive potential RNA-recognition motifs (RRMs) in the N-terminal followed by auxiliary domain in the C-terminal as similar with TIA-1 family, and to function as a modulator protein for transcripts by investigating the production of foreign recombinant proteins and transcripts in the Bombyx TRN-1 knock-down BmN4 cells (Kotani, et. al., Gene 320, 67-79, 2003). Subcellular localization and role of each domain of the Bombyx TRN-1 in the cultured cells has been investigated by the confocal fluorescence microscopy analyses on the cells over-expressing GFP-TRN-1 fusion protein and GFP-TRN-1 lacking C-terminal auxiliary domain and RRM domain in order to gain more insight into cellular function of the TRN-1. The recombinant GFP-fusion proteins with RRMs were indicated to assuredly have the poly (U) RNA binding activity. It was also shown that GFP-TRN-1 lacking C-terminal auxiliary domain and GFP-C-terminal domain were likely to be localized in the nucleus, while full-length GFP-TRN-1 was localized both in the nucleus and cytoplasm. However, clear localization of GFP-TRN-1 in the nucleus was observed after AcNPV was infected into the cells. In addition, it was indicated that GFP-TRN-1 over-expression could cause change of the recombinant baculovirus-produced luciferase activity during its infection, thus demonstrating that lepidopteran TRN-1 may control the function of both baculovirus and cellular transcripts in the response to stress caused by the viral infection.

Research Products

• [Journal Article] Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori. (2007)
  • Author(s): Arimatsu, Y., Kotani, E., Sugimura, Y., Furusawa, T.
  • Journal Title: Insect Biochemistry and Molecular Biology 37 Pages: 176-183
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyxmori. (2007)
  • Author(s): Arimatsu, Y., Sugimura, Y., Kotani, E, , Furusawa, T. 他5名7番目に記載
  • Journal Title: Journal of Insect Biotechnology and Sericology 76 Pages: 57-62
  • NAID: 10018743032
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and functional analysis of Relish homologs in the silkworm, Bombyx mori. (2007)
  • Author(s): Tanaka, H., Matsuki, H., Furukawa, S., Sagisaka, A., Kotani, E., Mori, H., Yamakawa, M.
  • Journal Title: Biochim. Biophys. Acta 1769 Pages: 559-568
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Nondestructive micro-patterning of proteinous occlusion bodies in water by femtosecond laser-induced mechanical forces. (2007)
  • Author(s): Hosokawa, Y., Kaji, T., Shukunami, C., Hiraki, Y., Kotani, E., Mori, H., Masuhara, H.
  • Journal Title: Biomed Microdevices 9 Pages: 105-111
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombvx mori (2007)
  • Author(s): Arimatsu, Y., Kotani, E., Sugimura, Y., Furusawa, T
  • Journal Title: Insect Biochem. Molec. Biol 37 Pages: 176-183
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyx mori (2007)
  • Author(s): Arimatsu, Y., Furuno, T., Sugimura, Y., Togoh, M., Ishihara, R., Tokizane, M., Kotani, E., Hayashi, Y., Furusawa, T
  • Journal Title: J. Insect Biotech. Sericol 76 Pages: 57-62
  • NAID: 10018743032
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nondestructive micro-patterning of proteinous occlusion bodies in water by femtosecond laser-induced mechanical forces (2007)
  • Author(s): Hosokawa, Y., Kaji, T., Shukunami, C., Hiraki, Y., Kotani, E., Mori, H., Masuhara, H
  • Journal Title: Biomed Microdevices 9 Pages: 105-111
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyx mori. (2007)
  • Author(s): Arimatsu, Y., Sugimura, Y., Kotani, E., Furusawa, T.他5名7番目に記載
  • Journal Title: Journal of Insect Biotechnology and Sericology 76 Pages: 57-62
  • NAID: 10018743032
  • Peer Reviewed
• [Journal Article] 昆虫においてタンパク質発現を調節しているRNA結合タンパク質 (2006)
  • Author(s): 小谷 英治
  • Journal Title: 蚕糸昆虫バイオテック 1 Pages: 22-23
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification and functional analysis of Relish homologs in the silkworm, Bombyx mori
  • Author(s): Tanaka, H., Matsuki, H., Furukawa, S., Sagisaka, A., Kotani, E., Mori, H., Yamakawa, M
  • Journal Title: Biochim. Biophys 1769 Pages: 559-568
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular characterization of the TIA-1 like RNA-binding protein, BmTRN-1 of the silkworm, Bombyx mori. (2008)
  • Author(s): E., Matsumoto, E., Kotani, et. al.
  • Organizer: Asia-Pacific congress of sericulture and insect Biotechnology 2008 Nagoya
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-22
• [Presentation] ハスモンヨトウ類細胞におけるRNA結合タンパク質(SfTRN-1)の発現解析 (2008)
  • Author(s): 武藤清佳, 小谷英治, ら,
  • Organizer: 平成20年度日本蚕糸学会78回大会-蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-20
• [Presentation] Molecular characterization of the TIA-1-like RNA-binding protein, BmTRN-1 of the silkworm, Bombyx mori. (2008)
  • Author(s): Matsumoto, E., Kotani, E., Mutoh, S., Tanabe, T., Saito, M., Mori, H.
  • Organizer: Asia-Pacific Congress of Sericulture and Insect Biotechnology
  • Place of Presentation: Nagoya University
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] (Kyoto Institute of Technology, Japan) : Molecular characterization of the TIA-1-like RNA-binding protein, BmTRN-1 of the silkworm (2008)
  • Author(s): Matsumoto, E., Kotani, E., Mutoh, S., Tanabe, T., Saito, M., Mori, H
  • Organizer: Bombyx mori. Asia-Pacific Congress of Sericulture and Insect Biotechnology
  • Place of Presentation: Nagoya University
  • Description: 「研究成果報告書概要(欧文)」より