Heavy-metal stress response system of plants, especially molecular-physiological analysis of detoxification mechanisms

• Project/Area Number: 18580064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Nihon University
• Principal Investigator: HASEGAWA Isao Nihon University, 生物資源科学部, 教授 (40218441)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 植物成長・生理 / 重金属ストレス応答 / シロイヌナズナ / カドミウム / フィトケラチン / グルタチオン / システイン / イオウ代謝系酵素遺伝子 / アブラナ科植物 / 強酸性 / 酵素遺伝子 / 他の重金属 / 定量PCR / CSase

Research Abstract

シロイヌナズナを用い、カドミウム(Cd)処理に伴う硫黄代謝系および各重金属リガンドの生合成系に関与する酵素遺伝子13個の発現量とリガンド生成量について検討したところ、Cd処理濃度で最も高発現している遺伝子は、フィトケラチン(PCs)合成酵素遺伝子(PCS1)であった。システイン合成能を強化した形質転換体ではPCS1遺伝子の高発現と同時にPCs含量が増加し、カドミウムによる生育抑制が顕著に軽減された。

Research Products

• [Journal Article] 植物におけるカドミウムの吸収・集積および耐性能に関する研究 (2008)
  • Author(s): 長谷川功
  • Journal Title: 土肥誌 79 Pages: 429-432
  • Peer Reviewed
• [Journal Article] Alkenyl group is responsible for the disruption of microtubule networl formation in human colon cancer cell line HT-29 cells (2008)
  • Author(s): T. Hosono, T. H. Fukao, K. Inada, R. Tanaka, I. Hasegawa, et.al.
  • Journal Title: Carcinogenesis 29 Pages: 1400-1406
  • Peer Reviewed
• [Journal Article] Aluminium distribution and reactive oxygen species accumulation in root tips of two Melaleuca trees differing in aluminium resistance (2008)
  • Author(s): K. Tahara, T. Yamanoshita, M. Norisada, I. Hasegawa, et.al.
  • Journal Title: Plant and Soil 307 Pages: 167-178
  • Peer Reviewed
• [Journal Article] 植物におけるカドミウムの吸収・集積および耐性能に関する研究 (2008)
  • Author(s): 長谷川功
  • Journal Title: 日本土壌肥料学雑誌 79 Pages: 429-432
  • Peer Reviewed
• [Journal Article] Alkenyl group is responsible for the disruption of microtubule networlformation in human colon cancer cell line HT-29 cells. (2008)
  • Author(s): T. Hosono, T. H. Fukao, K. Inada, R. Tanaka, I. Hasegawa, et al
  • Journal Title: Carcinogenesis 29 Pages: 1400-1406
  • Peer Reviewed
• [Journal Article] Aluminium distribution and reactive oxygen species accumulation inroot tips of two Melaleuca trees differing in aluminium resistance. (2008)
  • Author(s): K. Tahara, T. Yamanoshita, M. Norisada, I. Hasegawa, et al
  • Journal Title: Plant and Soil 307 Pages: 167-178
  • Peer Reviewed
• [Journal Article] Analysis of physiological characteristics of plants growing in acid sulfate soil: relationship between sulfur metabolism and stress tolerance in the plants. (2008)
  • Author(s): H. Kashima, F. Shinmachi, A. Noguchi, S. Sasaki, and I. Hasegawa
  • Journal Title: S. Sasaki eds. Development of New BioremediationSystems of Acid Sulfate Soil for Agriculture and Forestry, Shoukadoh Book Sellers, Kyoto, Japan Pages: 93-96
• [Journal Article] Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Viet Nam (2007)
  • Author(s): T. Aizawa, N. B. Ve, K. Kimoto, N. Iwabuchi, H. Sumida, I. Hasegawa, S. Sasaki, T. Tamura, T. Kudo, K. Suzuki, M. Nakajima, and M. Sunairi
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 57 Pages: 1447-1452
  • Peer Reviewed
• [Journal Article] 植物による環境浄化-ファイトレメディエーション技術開発の現状と今後の課題 (2007)
  • Author(s): 長谷川功
  • Journal Title: 農業および園芸 82(10) Pages: 1053-1061
  • NAID: 40015649468
  • Peer Reviewed
• [Journal Article] Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Viet Nam. (2007)
  • Author(s): T. Aizawa, N. B. Ve, K. Kimoto, N. Iwabuchi, H. Sumida, I. Hasegawa, S. Sasaki, T. Tamura, T. Kudo, K. Suzuki, M. Nakajima, and M. Sunairi.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 57 Pages: 1447-1452
  • Peer Reviewed
• [Journal Article] 植物による環境浄化-ファイトレメディエーション技術開発の現状と今後の課題 (2007)
  • Author(s): 長谷川 功
  • Journal Title: 農業および園芸 82(10) Pages: 1053-1061
  • NAID: 40015649468
  • Peer Reviewed
• [Journal Article] Curtobacterium ammoniigenes sp. Nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Viet Nam, (2007)
  • Author(s): T.Aizawa, N.B.Ve, K.Kimoto, N.Iwabuchi, H.Sumida, I.Hasegawa, S.Sasaki, T.Tamura, T.Kudo, K.Suzuki, M.Nakajima, S.Sunairi
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology (inpress)
• [Journal Article] Functional analysis of thiol compounds involved in sulfur metabolism during cadmium-ion stress in Arabidopsis thaliana. (2006)
  • Author(s): Atsuko Hiruta, Mio Watanabe, Mai Niikura, Sayuri Kurasako, Hiroyuki Kashima, Fumie Shinmachi, Akira Noguchi, Isao Hasegawa
  • Journal Title: Proceedings of 18^<th> World Congress of Soil Science Pages: 221-221
• [Journal Article] Functional analysis of adaptation of plants to strongly acidic soil. (2006)
  • Author(s): Hiroyuki Kashima, Hitomi Mase, Fumie Shinmachi, Akira Noguchi, Satohiko Sasaki, Isao Hasegawa
  • Journal Title: Proceedings of 18^<th> World Congress of Soil Science Pages: 220-220
• [Presentation] 植物の酸性適応能とリン栄養特性との関係 (2008)
  • Author(s): 鈴木彩子, 加島洋亨, 新町文絵, 野口章, 長谷川功
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] 強酸性耐性植物のアルミニウム応答特性について (2008)
  • Author(s): 寶満佳文, 加島洋亨, 田原恒, 新町文絵, 野口章, 長谷川功
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] カドミウムイオンに対する植物のストレス応答機構の解析 (2007)
  • Author(s): 蛭田温子、渡辺美生、今泉隆次郎、青木俊夫、野口章、長谷川功
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京農大
  • Year and Date: 2007-08-22
• [Presentation] カドミウムイオンに対する植物のストレス応答機構の解析 (2007)
  • Author(s): 蛭田温子、渡辺美生、今泉隆次郎、青木俊夫、野口 章、長谷川功
  • Organizer: 日本土壌肥料学会2007年度東京大会
  • Place of Presentation: 東京農業大学(東京)
  • Year and Date: 2007-08-22
• [Presentation] Functional analysis of thiol compounds involved in sulfur metabolism during cadmium-ion stress in Arabidopsis thaliana. (2006)
  • Author(s): Atsuko Hiruta, Mio Watanabe, Mai Niikura, Sayuri Kurasako, Hiroyuki Kashima, Fumie Shinmachi, Akira Noguchi, Isao Hasegawa
  • Organizer: Proceedings of 18^<th> World Congress of Soil Science,221
• [Presentation] Functional analysis of adaptation of plants to strongly acidic soil (2006)
  • Author(s): Hiroyuki Kashima, Hitomi Mase, Fumie Shinmachi, Akira Noguchi, Satohiko Sasaki, Isao Hasegawa
  • Organizer: Proceedings of 18^<th> World Congress of Soil Science, pp.220
• [Book] Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry (2008)
  • Author(s): H. Kashima, F. Shinmachi, A. Noguchi, S. Sasaki, I. Hasegawa, S. Sasaki et.al. (Eds.)
  • Publisher: Shoukadoh Book Sellers, Kyoto, Japan
• [Book] S. Sasaki et al(Eds. ) Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry (2008)
  • Author(s): H. Kashima, F. Shinmachi, A. Noguchi, S. Sasaki, I. Hasegawa
  • Publisher: Shoukadoh Book Sellers, Kyoto, Japan
• [Book] Overview and major outcomes in the plant science field.(S. Sasaki eds.)Development of New BioremediationSystems of Acid Sulfate Soil for Agriculture and Forestry, (2008)
  • Author(s): Isao Hasegawa
  • Total Pages: 150
  • Publisher: Shoukadoh Book Sellers, Kyoto, Japan
• [Book] 砂糖の科学 (2006)
  • Author(s): 長谷川功 他
  • Total Pages: 232
  • Publisher: 朝倉書店
• [Book] 肥料の事典 (2006)
  • Author(s): 長谷川功 他編著
  • Total Pages: 387
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://hp.brs.nihon-u.ac.jp/~lpnp/