| Project/Area Number |
18580065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
KANEKO Jun Tohoku University, Graduate School of Agricultural Science, Associate Prof (30221188)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIO Yoshiyuki Syokei Gakuin university, 健康栄養学科, Professor (00109175)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,940,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Staphylococcus aureus / Lipid raft / receptor / γ-hemolysin / leukocidin / γ-hemolysin / Staphycoccus aureus / lipid rafts / recepter |
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| Research Abstract |
The following points were clarified in research.
(1) The search for the factors interact with bi-component toxin: Membrane-pores of leukocidin (Luk) and γ-hemolysin (Hlg) were found being formed on lipid raft of the target cell membrane. In addition, LukS and leukocyte surface protein MD-2 were found interacting. On the other hand, at least two proteins obtained from the erythrocyte membrane were found to be interacting with LukF.
(2) The relationship between Luk and PVL and the inflammation response was analyzed: The chimera mutant of LukS and LukF-PV, and point mutants of LukS and LukS-PV were constructed. Rabbit model was used to assess the inflammation activities. On the other hand, the possibility that the Hlg/Luk each element takes part in the inducement of a natural immune response was found in the dendritic cell of the mouse marrow origin.
(3) The clarification of the expression regulator of the Hlg/Luk gene in the Staphylococcus aureus: It was clarified that the sae system that was one of the global regulators was taking part in the appearance of Hlg/Luk genes.
(4) The importance of bi-component toxin (especially, PVL) in the dermatology area: PVL producing strains were frequently isolated from furuncles and carbuncles in Japan. Moreover, the PVL producing strains was strains was found in the isolates from various peoples by routine bacterial test on admission.
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