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Localization mechanisms of Golgi peripheral membrane proteins

Research Project

Project/Area Number 18580068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

NODA Yoichi  The University of Tokyo, Graduate School of Agricultural And Life Sciences, Assistant (90282699)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsGolgi apparatus / vesicular transport / budding yeast / 応用微生物 / 蛋白質
Research Abstract

The Golgi apparatus consists of a set of vesicular compartments which are distinguished by their marker proteins. These compartments are physically separated in the yeast Saccharomyces cerevisiae cell. To characterize them extensively, we previously developed a method to isolate early Golgi compartments and late Golgi compartments separately, by immuonisolating vesicles carrying either of the SNAREs Sed5 or Tlg2, the markers of the early and late Golgi compartments, respectively, and analyzed the membrane-spanning proteins. Organelle identity of the Golgi apparatus is determined not only by the resident membrane-spanning proteins, but peripheral membranes, proteins also makes significant contributions to specify the functions of early or late Golgi subcompartment. Based on these facts, our goal in this study is to improve this immunoisolation procedure for the analysis of the peripheral Golgi membrane proteins and reveal the mechanism by which peripheral membrane proteins are recruited … More to the specific Golgi subcompartments. The proteins we mainly analyzed were Gea1 and Sec7, both of which are GDP/GTP exchange factors for the small G-protein Arf, but have distinct localizations with Gea1 being specifically localized to the early Golgi subcompartment and with Sec7 being specifically localized to the late Golgi subcompartment. We found, by making several modifications, that the immunoisolation procedure can be also used to determine localization of peripheral membrane proteins, as Gea1 was recovered in the Sed5-compartment (enriched in early Golgi proteins) and Sec7 was recovered in the Tlg2-compartment (enriched in late Golgi proteins). Amount of peripheral membrane proteins recovered by this procedure was further increased by altering buffers and mothods to disrupt cells, which made this immunoisolation method useful for comprehensive proteomics analysis of Golgi peripheral membrane proteins. Thus, we conclude that we successfully devised a simple method to analyse peripheral membrane proteins that reside on the yeast Golgi membranes. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (35 results)

All 2008 2007 2006 Other

All Journal Article (22 results) (of which Peer Reviewed: 11 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] KEGI/YFRO42w encodes a novel Kre6-binding ER membrane protein responsible for beta -1,6-glucan synthesis in Saccharomyces cerevisiae2007

    • Author(s)
      Kousuke Nakamata, Tomokazuk Kurita, M. Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 34315-34324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pgal is an essential component of Glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae.2007

    • Author(s)
      Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Journal Title

      Molecular Biology of the Cell 18

      Pages: 3472-3485

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Specific membrane recruitment of Usol protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport2007

    • Author(s)
      Yoichi Noda, Tomoe Yamagishi, Koji Yoda
    • Journal Title

      Journal of Cellular Biochemistry 101

      Pages: 686-694

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Saccharomyces cerevisiae BOR1p is a boron exporter and a key determinant of boron torelance2007

    • Author(s)
      Junpei Takano, Masaharu Kobayashi, Yoichi Noda and Toru Fujiwara
    • Journal Title

      FEMS Microbiology Letters 267

      Pages: 230-235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp52007

    • Author(s)
      Keita Imai, Yoichi Noda, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Biosci. Biotechnol. Biochem 71

      Pages: 249-252

    • NAID

      10027510698

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae2007

    • Author(s)
      Hironori Inadome, Yoichi Noda, Yurika Kamimura, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Exp. Cell. Res 313

      Pages: 688-697

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tvp38, Tvp23, Tvp18 and Tvp15 : novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae2007

    • Author(s)
      Hironori Inadome, Yoichi Noda, Yurika Kamimura, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Exp. Cell. Res 313(4)

      Pages: 688-697

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp52007

    • Author(s)
      Keita Imai, Yoichi Noda, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Biosci. Biotechnol. Biochem 71(1)

      Pages: 249-252

    • NAID

      10027510698

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pgal is an essential component of Glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae2007

    • Author(s)
      Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Journal Title

      Mol. Biol. Cell 18(9)

      Pages: 3472-3485

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Specific membrane recruitment of Usol protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport2007

    • Author(s)
      Yoichi Noda, Tomoe Yamagishi, Koji Yoda
    • Journal Title

      J. Cell. Biochem 101(3)

      Pages: 686-694

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] KEG1/YFR042w encodes a novel Kre6-binding ER membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae2007

    • Author(s)
      Kousuke Nakamata, Tomokazu Kurita, M. Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Journal Title

      J. Biol. Chem 282(47)

      Pages: 34315-34324

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] KEG1/YFRO42w encodes a novel Kre6-binding ER membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae2007

    • Author(s)
      Kousuke Nakamata, Tomokazu Kurita, M. Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 34315-34324

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pga1 is an essential component of Glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae.2007

    • Author(s)
      Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Journal Title

      Molecular Biology of the Cell 18

      Pages: 3472-3485

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Specific Membrane Recruitment of Uso1 Protein, theEssential Endoplasmic Reticulum-to-Golgi Tethering Factor in Yeast Vesicular Transport2007

    • Author(s)
      Yoichi Noda, Tomoe Yamagishi, Koji Yoda
    • Journal Title

      Journal of Cellular Biochemistry (Published on line)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Saccharomyces cerevisiae Bor1p is a boron exporter and a key determinant of boron tolerance2007

    • Author(s)
      Junpei Takano, Masaharu Kobayashi, Yoichi Noda, Toru Fujiwara
    • Journal Title

      FEMS Microbiology Letters 267

      Pages: 230-235

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp52007

    • Author(s)
      Imai Keita, Yoichi Noda, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Biosci. Biotechnol. Biochen. 71

      Pages: 249-252

    • NAID

      10027510698

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular cloning and characterization of a Pichia pastoris ortholog of_the yeast Golgi GDP-mannose transporter gene.2006

    • Author(s)
      Kumiko Arakawa, Masato Abe, Yoichi Noda, Hiroyuki Adachi and Koji Yoda.
    • Journal Title

      J. Gen. Appl. Microbiol 52

      Pages: 137-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Isolation and structural analysis of efficient autonomously replicating sequences (ARSs) of the yeast Candida utilis2006

    • Author(s)
      Ryo Iwakiri, Shogo Eguchi, Yoichi Noda, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Yeast 23

      Pages: 23-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tvp38, Tvp23, Tvp18 and Tvp15 : Novel membrane proteins in the Tlg2- containing Golgi/endosome compartments of Saccharomyces cerevisiae2006

    • Author(s)
      Hironori Inadome, Yoichi Noda, Yurika Kamimura, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      Experimental Cell Research 313

      Pages: 688-697

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular cloning and characterization of a Pichia pastoris ortholog of the yeast Golgi GDP-mannose transporter gene2006

    • Author(s)
      Kumiko Arakawa, Masato Abe, Yoichi Noda, Hiroyuki Adachi, Koji Yoda
    • Journal Title

      J. Gen. Appl. Microbiol. 52

      Pages: 137-145

    • NAID

      130004802064

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The Rab GTPase Ypt11 functions in bud-directed transport of the Golgi by linking Myo2 to the coatomer subunit Ret2

    • Author(s)
      Seisuke Arai, Yoichi Noda, Satoko Kainuma, Ikuo Wada, and Koji Yoda
    • Journal Title

      Current Biology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The Rab GTPase Ypt11 functions in bud-directed transport of the Golgi by linking Myo2 to the coatomer subunit Ret2

    • Author(s)
      Seisuke Arai, Yoichi Noda, Satoko Kainuma, Ikuo Wada, and Koji Yoda
    • Journal Title

      Curr. Biol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Saccharomyces cerevisiaeのβ-1,6-グルカン合成に関わる必須遺伝子KEGIの解析2008

    • Author(s)
      栗田朋和, 中俣浩介, M. Shah Alam Bhuiyan, 佐藤啓介, 野田陽一, 依田幸司
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] KEG1/YFR042w encodes a novel Kre6-binding ER membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae2008

    • Author(s)
      Tomokazu Kurita, Kousuke Nakamata, M. Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Organizer
      Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pgal is an essential component of Glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae2007

    • Author(s)
      Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Organizer
      XXIIIrd International Conference on Yeast Geneti cs and Molecular Biology
    • Place of Presentation
      メルボルン
    • Year and Date
      2007-07-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pga1 is an essential component of Glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae.2007

    • Author(s)
      Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Organizer
      XXIIIrd International Conference on Yeast Genetics and Molecular Biology
    • Place of Presentation
      メルボルン
    • Year and Date
      2007-07-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] 出芽酵母ゴルジ体膜タンパク質Svp26はマンノース転移酵素の局在に関与する2007

    • Author(s)
      野田陽一, 依田幸司
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 出芽酵母Ypt11タンパク質のゴルジ体分配における役割2007

    • Author(s)
      荒井斉祐、野甲陽一、依田幸司
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Yeast protein Svp26 functions to localize mannosyltransferase to the Golgi2007

    • Author(s)
      Yoichi Noda and Koji Yoda
    • Organizer
      Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of Ypt11 function in Golgi inheritance2007

    • Author(s)
      Seisuke Arai, Yoichi Noda and Koji Yoda
    • Organizer
      Yeast Genetics Society of Japan, the 39rd meeting
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pga1 is an essential component of Glycosylphosphatidylinositol-Mannosyltransferase II of Saccharomyces cerevisiae2007

    • Author(s)
      Keisuke Sato, Yoichi Noda, and Koji Yoda
    • Organizer
      XXIIIrd International Conference on Yeast Genetics and Molecular Biology, Melbourne, Australia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 出芽酵母Ypt11タンパク質のゴルジ体分配における機能2006

    • Author(s)
      荒井斉祐、野田陽一、依田幸司
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      三島
    • Year and Date
      2006-07-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 輸送経路で働く新たな膜蛋白質の発見と機能解析2006

    • Author(s)
      野田陽一
    • Organizer
      酵母合同シンポジウム
    • Place of Presentation
      山梨
    • Year and Date
      2006-06-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Ypt11 functions to deliver the late Golgi to the bud2006

    • Author(s)
      Seisuke Arai, Yoichi Noda and Koji Yoda
    • Organizer
      Yeast Genetics Society of Japan, the 39 meeting
    • Place of Presentation
      Mishima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://park.itc.u-tokyo.ac.jp/molbiotech/

    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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