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Building up a network of degradation enzymes in a thermophilic bacterium that degrade aromatic compounds

Research Project

Project/Area Number 18580077
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionOkayama University

Principal Investigator

KINBARA Kazuhide  Okayama University, 資源生物科学研究所, 准教授 (30225122)

Co-Investigator(Kenkyū-buntansha) TANI Akio  岡山大学, 資源生物科学研究所, 助教 (00335621)
河合 富佐子  岡山大学, 資源生物科学研究所, 教授 (60118007)
清水 頼子  岡山大学, 資源生物科学研究所, 技術職員 (70379808)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywords芳香族化合物 / 好熱性細菌 / 分解 / 代謝酵素 / ナフタレン / 分解酵素 / 遺伝子群 / 進化 / 微生物分解 / 代謝産物 / 生理変化 / プロテオミクス
Research Abstract

芳香族化合物を分解する微生物は、その能力を進化の過程で獲得してきたものと考えられている。本研究では、これまで解析された例が少ない好熱性細菌に着目し、ナフタレン代謝酵素遺伝子群の解析を行うことを目的とした。その結果、遺伝子の単離とナフタレンによる誘導性を見出すことが出来た。しかし、ナフタレンを代謝する過程で生理活性が著しく低下することが分かり、酵素機能と代謝ネットワークの詳細を解析するにはいたらなかった。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (7 results)

All 2009 2008 2007 2006

All Journal Article (1 results) Presentation (6 results)

  • [Journal Article] 環境微生物の生き様を探る2007

    • Author(s)
      金原和秀
    • Journal Title

      化学と生物 45

      Pages: 869-875

    • NAID

      10019973674

    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Detection and assessment of physiological activity of thermophilic bacteria during aromatic compounds degradation2009

    • Author(s)
      Kimbara, K., Iijima, S., Kanesaka, T., and Tani A.
    • Organizer
      JSPS-NRCT Asian Core Program Joint Seminar
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 環境細菌DNAのダイナミズム2008

    • Author(s)
      金原和秀・田中昭行・松井一泰・新谷正巳・野尻秀昭
    • Organizer
      細菌研究会
    • Place of Presentation
      長津田
    • Year and Date
      2008-06-14
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 芳香族化合物が好熱菌の生育に及ぼす影響の解析2007

    • Author(s)
      金坂貴志, 下村有美, 河合富佐子, 金原和秀
    • Organizer
      日本農芸化学会中四国支部第17回講演会
    • Place of Presentation
      香川
    • Year and Date
      2007-01-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Detection and assessment of physiological change and cellular oxidation of bacteria by aromatic compounds and metals2007

    • Author(s)
      Kimbara, K., and Mori, I. C.
    • Organizer
      13^<th> International Symposium on Toxicity Assessment
    • Place of Presentation
      Toyama, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Detection and assessment of physiological change and cellular oxidation of bacteria by aromatic compounds and metals2007

    • Author(s)
      (2)Kimbara, K., and Mori, I. C.
    • Organizer
      13th International Symposium on Toxicity Assessment
    • Place of Presentation
      Toyama, Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of genes for naphthalene degradation in the thermophilic naphthalene and PCB degrader2006

    • Author(s)
      Miyazawa, D., Hatta, T. and Kimbara, K
    • Organizer
      Bacillus sp. JF8
    • Place of Presentation
      The 5th JSPS-NRCT Joint Seminar, Pattaya, Thailand
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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