| Budget Amount *help |
¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
The objectives of this research are to discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide and hydrogen peroxide breakdown, and to accelerate its degrading capability being formed in the intestine.
In 2005, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of hydrogen peroxide. In 2006 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.
After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened a total ofl00 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 10 out 100 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and Phen
… More
otypic Analysis. As a result of the identification, they are classified as families of Lactobacillus plantarum and Pediococcus pentosaceus.
For measuring degradation activity of lipoperoxide and hydrogen peroxide, we prepared for Lactobacillus Standard GYP medium, and conditioned with aerobic culture at 37□ and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide and Pediococcus pentosaceus Be-I performed the highest one of hydrogen peroxide. Pediococcus pentosaceus Be-I can scavenge both cumene peroxide and hydrogen peroxide.
In presence of butyl peroxide for identifying reaction product of L. plantarum Panl-2 and P pentosaceus Be-I, the strains of resting cell showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strains have 2-electron reduction of peroxide. Less
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