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Proteomic identification and functional analysis of novel biodefensive factors in milk and the mammary gland in early involution

Research Project

Project/Area Number 18580090
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionNagoya University

Principal Investigator

DAITA Nadano  Nagoya University, Graduate Schcol of Bioagricultural Sciences, Associate Professor (00228074)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,980,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsapplied bioregulation / body defense / antioxidant activity / mammary gland / involution / milk / proteomics / breast cancer / 乳清 / 網羅的解析 / マウス
Research Abstract

The mammary gland develops mainly after birth, and shows a repeated cycle of pregnancy-triggered proliferation, differentiation for lactation, and a regressive phase after weaning known as involution. As compared to the proliferation and differentiation phases, the molecular mechanisms of involution are largely unknown. In the present study we screened genes that could play a potential role in early involution of the mouse mammary gland at the message level. We found that new genes were unregulated more than two-fold 48 h after weaning. The products of these genes can be linked to defense against oxidative stress and/or infectious bacteria In addition to the analysis at the mRNA level, we performed proteomic analysis of milk proteins, especially milk serum proteins which have been considered to possess multiple activities potentially useful for human health Milk serum proteins were separated from whole milk of mice by combination of centrifugations and separated by one/two-dimensional gel electrophoresis. Protein bands and spots thus obtained were subjected to matrix-assisted laser-desorption time-of-flight and tandem mass spectrometry. Proteins related to the breast cancer were also analyzed. About 100 proteins were identified from the highly purified milk serum samples. Our analysis showed that various biodefensive fedora in milk increased within 48 h after weaning. These increases were in contrast to the constant presence of other major proteins including albumin, caseins, transferrin, and whey acidic protein. The identified proteins may cooperate for the defense of the mammary gland in a post-lactation period

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2007 2006 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Remarks (2 results)

  • [Journal Article] Bystin in human cancer cells: intracellular localization and function in ribosome biogenesis2007

    • Author(s)
      Masaya, M.(第一著者)
    • Journal Title

      Biochemical Journal 404

      Pages: 373-381

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bystin in human cancer cells : intracellular localization and function in ribosome biogenesis2007

    • Author(s)
      Miyoshi, M., Okajima, T., Matsuda, T., Fukuda, M. N., Nadano, D
    • Journal Title

      Biochemical Journal 404-3

      Pages: 373-381

    • NAID

      120006406625

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bystin in human cancer cells: intracellular localization and function in ribosome bioeenesis.2007

    • Author(s)
      Masaya, M.(第一著者)
    • Journal Title

      Biochemical Journal 404

      Pages: 373-381

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidant and antibacterial genes are upregulated in early involution of the mouse mammary gland: sharp increase of ceruloplasmin and lactoferrin in accumulating breast milk.2006

    • Author(s)
      Nakamura, M.(第一著者)
    • Journal Title

      DNA and Cell Biology 25

      Pages: 491-500

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Antioxidant and antibacterial genes are upregulated in early involution of the mouse mammary gland : sharp increase of ceruloplasmin and lactoferrin in accumulating breast milk2006

    • Author(s)
      Nakamura, M., Tomita, A, Nakatani, H, Matsuda, T., Nadano, D
    • Journal Title

      DNA and Cell Biology 25-9

      Pages: 491-500

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Antioxidant and antibacterial genes are upregulated in early involution of the mouse mammary gland : sharp increase of ceruloplasmin and lactoferrin in accumulating breast milk.2006

    • Author(s)
      Nakamura, M.(第一著者)
    • Journal Title

      DNA and Cell Biology 25・9

      Pages: 491-500

    • Related Report
      2006 Annual Research Report
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.agr.nagoya-u.ac.jp/%7emolreg00/cancer.htm

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/%7emolreg00/cancer.htm

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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