| Project/Area Number |
18580098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Sojo University |
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Principal Investigator |
ODA Naoko Sojo University, Faculty of Pharmaceutical Sciences, Professor (70211828)
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| Co-Investigator(Kenkyū-buntansha) |
KARIU Tohru Sojo University, Faculty of Pharmaceutical Sciences, Lecturer (10412735)
TAKAZAKI Shinya Sojo University, Faculty of Pharmaceutical Sciences, Research assistant (90435149)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,970,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | venom / transcription / ハブ(ヘビ) / ハブ / 発現調節 / 毒素 |
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| Research Abstract |
Protobothrops flavoviridis snakes inhabit the southwestern islands of Japan: Amami-Oshima, Tokunoshima and Okinawa. P flavoviridis venom contains abundant biologically active proteins. In order to understand molecular mechanisms of transcription and translation in P. flavoviridis venom gland, the following two subjects have been studied. 1) Transcriptomics of Amami-Oshima P. flavoviridis venom gland, and proteomics of P flavoviridis venoms from Amami-Oshima, Tokunoshima and Okinawa. The detailed study on transcriptomics of P. flavoviridis venom gland led to the finding of several novel genes participating in transcription and translation in addition to toxic protein transcripts. The comparative proteomics study on P. flavoviridis venoms from the three islands by means of two dimensional gel electrophoresis and ESI-Q-TOF mass spectrometry disclosed that island specific expression or mutation have occurred in toxic proteins in individual islands. 2) An attempt to identify venom gland specific transcription factors in P flavoviridis. Five promotor sequences of about 40 by lengths of the genes encoding venom proteins were chosen and tested by means of gel-shift assay whether they are able to interact with protein (s) in P flavoviridis venom gland extracts. One candidate was verified to bind to a protein from the extracts. Identification of this protein is under way using ESI-Q-TOF analysis.
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