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Elucidation of mechanisms regulating expression of a phytoalexin biosynthetic gene cluster in rice

Research Project

Project/Area Number 18580102
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

YAMANE Hisakazu  The University of Tokyo, Biotechnology Research Center, Professor (80090520)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsOryza sativa L. / phytoalexins / momilactones / gene cluster / chitin oligosaccharide elicitor / suspension-cultured cells / P450 / master transcription factor / 生合成遺伝子クラスター / エリシター / ファイトカサン / 転写因子 / ファイトアレキシン生合成 / ジテルペン環化酵素遺伝子 / bZIP型転写因子 / キチンエリシター / Tos17挿入変異株
Research Abstract

We found that two P450 genes (CYP99A2 and CYP99A3) and a putative dehydrogenase gene (AK103462) forms a gene cluster on chromosome 4 of the rice genome together with two diterpene cyclase genes that are involved in biosynthesis of momilactones. In addition, the five clustered genes were coordinately induced after treatment with a chitin oligosaccharide elicitor in suspension-cultured rice cells, suggesting that the momilactone biosynthesis genes are clustered on chromosome 4.
On the basis of the above background, this project aimed to demonstrate the presence of a momilactone biosynthetic gene cluster and elucidate regulatory mechanisms for coordinate expression of the gene cluster. We indicated that AK103462 encodes momilactone A synthase, and that the double knockdown of CYP99A2 and CYP99A3 specifically suppressed the elicitor inducible production of momilactones. We thus demonstrated that a momilactone biosynthetic gene cluster consisting of four or five genes is present on chromosome 4 in rice. In addition, we identified an elicitor-inducible basic leucine zipper (bZIP) transcription factor, OsTGAP1, which coordinately regulates the expression of all five genes in the momilactone biosynthetic gene cluster. We also showed that OsTGAP1 can influence the expression of a phytocassane biosynthetic gene and an upstream methylerythritol phosphate (MEP) pathway gene. We thus indicated that OsTGAP1 functions as a key regulator of the coordinated transcription of genes involved in inductive diterpenoid phytoalexins production in rice.
All the studies presented here give us important basic information to clarify regulatory mechanisms for production of diterpenoid phytoalexins in rice.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (30 results)

All 2009 2008 2007 2006 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (25 results)

  • [Journal Article] OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice in rice2009

    • Author(s)
      Okada, Yamane, et. al.
    • Journal Title

      J.Biol.Chem. 284(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of a biosynthetic gene cluster in rice for momilactones2007

    • Author(s)
      Shimura, Yamane, et. al.
    • Journal Title

      J.Biol.Chem. 282

      Pages: 34013-34018

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of a biosynthetic gene cluster in rice for momilactones2007

    • Author(s)
      K.Shimura, A.Okada, K.Okada, Y.Jikumaru, K.Ko, T.Toyomasu, T.Sassa, M.Hasegawa, O.Kodama, N.Shibuya, J.Koga, H.Nojiri, H.Yamane
    • Journal Title

      J.Biol.Chem. 282

      Pages: 34013-34018

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of a biosynthetic gene cluster in rice for momilactones2007

    • Author(s)
      Shimura, et. al.
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 34013-34018

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice

    • Author(s)
      A.Okada, K.Okada, K.Miyamoto, J.Koga, N.Shibuya, H.Nojiri, H.Yamane
    • Journal Title

      J.Biol.Chem. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] bZIP型転写因子OsTGAPによるイネモミラクトン生合成遺伝子クラスターの同調的な発現制御2009

    • Author(s)
      岡田敦、山根久和, ら
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] OsTGAP1, a bZIP transcription factor, regulates cluster-scale inducible gene expression for biosynthesis of diterpenoid phytoalexin in rice2009

    • Author(s)
      Miyamoto, K., Yamane, H., et. al.
    • Organizer
      Terpnet 2009
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The presence of diterpenoid phytoalexin biosynthetic gene clusters containing cytochrome P450 monooxygenase genes in rice2009

    • Author(s)
      Shimura, K., Yamane, H., et. al.
    • Organizer
      16^th International Conference on Cytochrome P450
    • Place of Presentation
      Okinawa
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] OsTGAPI, a bZIP transcription factor, regulates cluster-scale inducible gene expression for momilactone biosynthesis in rice2009

    • Author(s)
      Miyamoto, K., Yamane, H., et. al.
    • Organizer
      XIV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Quebec City, Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of a novel gene cluster in rice for diterpenoid Phytoalexin biosynthesis2009

    • Author(s)
      Minoda, H., Yamane, H., et. al.
    • Organizer
      XIV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Quebec City, Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of coordinated expression of momilactone biosynthetic gene cluster in rice2009

    • Author(s)
      Okada, A., Okada, K., Shibuya, N., Koga, J., Nojiri, H., Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Plant Physiologists
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The presence of diterpenoid phytoalexin biosynthetic gene clusters containing cytochrome P450 monooxygenase genes in rice2009

    • Author(s)
      Shimura, K., Minoda, H., Okada, A., Okada, K., Toyomasu, T., Koga, J., Shibuya, N., Yajima, A., Yabuta, G., Nojiri, H., Yamane, H.
    • Organizer
      16^<th> International Conference on Cytochrome P450
    • Place of Presentation
      Okinawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] OsTGAP1, a bZIP transcription factor, regulates cluster-scale inducible gene expression for momilactone biosynthesis in rice2009

    • Author(s)
      Miyamoto, K., Okada, A., Okada, K., Koga, J., Shibuya, N., Nojiri, H., Yamane, H.
    • Organizer
      XIV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Quebec City, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of a novel gene cluster in rice for diterpenoid phytoalexin biosynthesis2009

    • Author(s)
      Minoda, H., Okada, A., Okada, K., Koga, J., Shibuya, N., Toyomasu, T., Yajima, A., Yabuta, G., Nojiri, H., Yamane, H.
    • Organizer
      XIV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Quebec City, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネのジテルペン型ファイトアレキシン、モミラクトン類生合成酵素遺伝子発現制御へのbZIP型転写因子OsTGA1の関与2008

    • Author(s)
      岡田敦、山根久和, ら
    • Organizer
      植物化学調節学会43回大会
    • Place of Presentation
      つくば
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Involvement of the bZIP transcription factor OsTGAP1 in regulation of expression of momilactone biosynthetic genes in rice2008

    • Author(s)
      Okada, A., Okada, K., Shibuya, N., Koga, J., Nojiri, H., Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Chemical Regulation of Plants
    • Place of Presentation
      Tsukuba
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネのジテルペン系ファイトアレキシンの生合成2007

    • Author(s)
      山根久和
    • Organizer
      藪田セミナー
    • Place of Presentation
      東京
    • Year and Date
      2007-05-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネのファイトアレキシン生産に関与するジテルペン環化酵素遺伝子OsKS4の発現を制御するbZIP型転写因子の探索2007

    • Author(s)
      岡田敦、山根久和, ら
    • Organizer
      第48回日本植物生理学会年会
    • Place of Presentation
      松山
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネのエリシター応答性bZIP型転写因子のジテルペン環化酵素遺伝子OsKS4の発現制御への関与2007

    • Author(s)
      岡田敦、山根久和, ら
    • Organizer
      日本農芸化学会大会2007
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] OsTGA1, a bZIP transcriptional factor, regulates expression of the diterpene cyclase gene OsKS4 involved in production of phytoalexins in rice2007

    • Author(s)
      Okada, A, Yamane, H., et. al.
    • Organizer
      The 19^th International Conference on Plant Growth Substances
    • Place of Presentation
      Puerto Pallarta, Mexico
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Elicitor-induced defense response and their regulatory mechanisms in rice2007

    • Author(s)
      Okada, A., Yamane, H.
    • Organizer
      The University of Tokyo International SymPosium "Frontier of Microbial and Plant Biotechnology in Environmental and Life Sciences"
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Coordinate expression of genes clustered on rice chromosome 4 for the biosynthesis of momilactone2007

    • Author(s)
      Okada, K., Yamane, H., et. al.
    • Organizer
      The University of Tokyo International Symposium "Frontier of Microbial and Plant Biotechnology in Environmental and Life Sciences"
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] OsTGA1, a bZIP transcriptional factor, regulates expression of the diterpene cyclase gene OsKSL4 involved in production of phytoalexins in rice2007

    • Author(s)
      Okada, A., Yamane, H., et. al.
    • Organizer
      The University of Tokyo International Symposium "Frontier of Microbial and Plant Biotechnology in Environmental and Life Sciences"
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Survey of a bZIP transcriptional factor that regulates expression of the diterpene cyclase gene OsKS4 involved in production of phytoalexins in rice2007

    • Author(s)
      Okada, A., Nagamura, Y., Okada, K., Shibuya, N., Nojiri, H., Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Plant Physiologists
    • Place of Presentation
      Matsuyama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Involvement of an elicitor-responsive bZIP transcriptional factor in regulation of expression of the diterpene cyclase gene OsKS4 in rice2007

    • Author(s)
      Okada, A., Nagamura, Y., Okada, K., Shibuya, N., Nojiri, H., Yamane, H
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] OsTGA1, a bZIP transcriptional factor, regulates expression of the diterpene cyclase gene OsKS4 involved in production of phytoalexins in rice2007

    • Author(s)
      Okada, A., Okada, K., Nagamura, Y., Toyomasu, T., Koga, J., Shibuya, N., Nojiri, H., Yamane, H.
    • Organizer
      The 19^<th> International Conference on Plant Growth Substances
    • Place of Presentation
      Puerto Pallarta, Mexico
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Biosynthesis of diterpenoid phytoalexins in rice2007

    • Author(s)
      Yamane, H.
    • Organizer
      Yabuta Seminar on biosynthesis of terpenoid secondary metabolites in microorganisms and plants
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Elicitor-induced defense response and their regulatory mechanisms in rice2007

    • Author(s)
      Yamane, H., Okada, K.
    • Organizer
      The University of Tokyo International Symposium "Frontier of Microbial and Plant Biotechnology in Environmental and Life Sciences"
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イネにおけるファイトアレキシン生合成遺伝子クラスターの解析2006

    • Author(s)
      志村和弘、山根久和, ら
    • Organizer
      植物化学調節学会第41回大会
    • Place of Presentation
      堺、大阪
    • Year and Date
      2006-10-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of gene clusters involved in biosynthesis of diterpenoid phytoalexins in rice2006

    • Author(s)
      Shimura, K., Okada, A., Jikumaru, Y., Otomo, K., Toyomasu, T., Mitsuhashi, W., Sassa, T., Hasegawa, M., Kodama, O., Shibuya, N., Okada, K., Nojiri, H., Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Chemical Regulation of Plants
    • Place of Presentation
      Sakai, Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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