Elucidation of mechanisms regulating expression of a phytoalexin biosynthetic gene cluster in rice
| Project/Area Number |
18580102
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMANE Hisakazu The University of Tokyo, Biotechnology Research Center, Professor (80090520)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Oryza sativa L. / phytoalexins / momilactones / gene cluster / chitin oligosaccharide elicitor / suspension-cultured cells / P450 / master transcription factor / 生合成遺伝子クラスター / エリシター / ファイトカサン / 転写因子 / ファイトアレキシン生合成 / ジテルペン環化酵素遺伝子 / bZIP型転写因子 / キチンエリシター / Tos17挿入変異株 |
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| Research Abstract |
We found that two P450 genes (CYP99A2 and CYP99A3) and a putative dehydrogenase gene (AK103462) forms a gene cluster on chromosome 4 of the rice genome together with two diterpene cyclase genes that are involved in biosynthesis of momilactones. In addition, the five clustered genes were coordinately induced after treatment with a chitin oligosaccharide elicitor in suspension-cultured rice cells, suggesting that the momilactone biosynthesis genes are clustered on chromosome 4.
On the basis of the above background, this project aimed to demonstrate the presence of a momilactone biosynthetic gene cluster and elucidate regulatory mechanisms for coordinate expression of the gene cluster. We indicated that AK103462 encodes momilactone A synthase, and that the double knockdown of CYP99A2 and CYP99A3 specifically suppressed the elicitor inducible production of momilactones. We thus demonstrated that a momilactone biosynthetic gene cluster consisting of four or five genes is present on chromosome 4 in rice. In addition, we identified an elicitor-inducible basic leucine zipper (bZIP) transcription factor, OsTGAP1, which coordinately regulates the expression of all five genes in the momilactone biosynthetic gene cluster. We also showed that OsTGAP1 can influence the expression of a phytocassane biosynthetic gene and an upstream methylerythritol phosphate (MEP) pathway gene. We thus indicated that OsTGAP1 functions as a key regulator of the coordinated transcription of genes involved in inductive diterpenoid phytoalexins production in rice.
All the studies presented here give us important basic information to clarify regulatory mechanisms for production of diterpenoid phytoalexins in rice.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Identification of a biosynthetic gene cluster in rice for momilactones2007
Author(s)
K.Shimura, A.Okada, K.Okada, Y.Jikumaru, K.Ko, T.Toyomasu, T.Sassa, M.Hasegawa, O.Kodama, N.Shibuya, J.Koga, H.Nojiri, H.Yamane
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Journal Title
J.Biol.Chem. 282
Pages: 34013-34018
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Regulation of coordinated expression of momilactone biosynthetic gene cluster in rice2009
Author(s)
Okada, A., Okada, K., Shibuya, N., Koga, J., Nojiri, H., Yamane, H.
Organizer
Annual Meeting of the Japanese Society for Plant Physiologists
Place of Presentation
Nagoya
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The presence of diterpenoid phytoalexin biosynthetic gene clusters containing cytochrome P450 monooxygenase genes in rice2009
Author(s)
Shimura, K., Minoda, H., Okada, A., Okada, K., Toyomasu, T., Koga, J., Shibuya, N., Yajima, A., Yabuta, G., Nojiri, H., Yamane, H.
Organizer
16^<th> International Conference on Cytochrome P450
Place of Presentation
Okinawa
Description
「研究成果報告書概要(欧文)」より
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[Presentation] OsTGAP1, a bZIP transcription factor, regulates cluster-scale inducible gene expression for momilactone biosynthesis in rice2009
Author(s)
Miyamoto, K., Okada, A., Okada, K., Koga, J., Shibuya, N., Nojiri, H., Yamane, H.
Organizer
XIV International Congress on Molecular Plant-Microbe Interactions
Place of Presentation
Quebec City, Canada
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of a novel gene cluster in rice for diterpenoid phytoalexin biosynthesis2009
Author(s)
Minoda, H., Okada, A., Okada, K., Koga, J., Shibuya, N., Toyomasu, T., Yajima, A., Yabuta, G., Nojiri, H., Yamane, H.
Organizer
XIV International Congress on Molecular Plant-Microbe Interactions
Place of Presentation
Quebec City, Canada
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Involvement of the bZIP transcription factor OsTGAP1 in regulation of expression of momilactone biosynthetic genes in rice2008
Author(s)
Okada, A., Okada, K., Shibuya, N., Koga, J., Nojiri, H., Yamane, H.
Organizer
Annual Meeting of the Japanese Society for Chemical Regulation of Plants
Place of Presentation
Tsukuba
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Survey of a bZIP transcriptional factor that regulates expression of the diterpene cyclase gene OsKS4 involved in production of phytoalexins in rice2007
Author(s)
Okada, A., Nagamura, Y., Okada, K., Shibuya, N., Nojiri, H., Yamane, H.
Organizer
Annual Meeting of the Japanese Society for Plant Physiologists
Place of Presentation
Matsuyama
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Involvement of an elicitor-responsive bZIP transcriptional factor in regulation of expression of the diterpene cyclase gene OsKS4 in rice2007
Author(s)
Okada, A., Nagamura, Y., Okada, K., Shibuya, N., Nojiri, H., Yamane, H
Organizer
Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
Place of Presentation
Tokyo
Description
「研究成果報告書概要(欧文)」より
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[Presentation] OsTGA1, a bZIP transcriptional factor, regulates expression of the diterpene cyclase gene OsKS4 involved in production of phytoalexins in rice2007
Author(s)
Okada, A., Okada, K., Nagamura, Y., Toyomasu, T., Koga, J., Shibuya, N., Nojiri, H., Yamane, H.
Organizer
The 19^<th> International Conference on Plant Growth Substances
Place of Presentation
Puerto Pallarta, Mexico
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Characterization of gene clusters involved in biosynthesis of diterpenoid phytoalexins in rice2006
Author(s)
Shimura, K., Okada, A., Jikumaru, Y., Otomo, K., Toyomasu, T., Mitsuhashi, W., Sassa, T., Hasegawa, M., Kodama, O., Shibuya, N., Okada, K., Nojiri, H., Yamane, H.
Organizer
Annual Meeting of the Japanese Society for Chemical Regulation of Plants
Place of Presentation
Sakai, Osaka
Description
「研究成果報告書概要(欧文)」より
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