Elucidation of molecular mechanism of plant eavesdropping (perception of volatile compounds-An approach for a novel plant regulator-

• Project/Area Number: 18580105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Yamaguchi University
• Principal Investigator: MATSUI Kenji Yamaguchi University, 大学院・医学系研究科, 教授 (90199729)
• Co-Investigator(Kenkyū-buntansha): MANO Jun'ichi 山口大学, 総合科学実験センター, 准教授 (50243100) ; OGAWA Ken'ichi 岡山県生物科学総合研究所, 細胞工学部門, 室長 (70344405)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 植物 / 揮発性化合物 / 生理活性 / 化学生態学 / 立ち聞き / 匂い受容 / 生理活性揮発成分 / 立ち聞き現象 / 生活活性 / 生態学

Research Abstract

シロイヌナズナ葉を(E)-2-ヘキセナール処理すると、NADPHオキシダーゼ特異的に過酸化水素の蓄積がおこり、これが引き金となって細胞膜カルシウムチャネルが開口し、カルシウムイオンの流入が引き起こされることを明らかとした。その後、還元型グルタチオン量が一過的に減少し、こうした一連の応答が防御遺伝子誘導につながることが示唆された。またメチルビニルケトンなどのα,β-不飽和カルボニル化合物が同様の機構で植物をプライミングすることが示され、新規植物化学調節物質としての可能性を提唱することができた

Research Products

• [Journal Article] みどりの香りを介した生物間相互作用 (2009)
  • Author(s): 松井健二、杉本貢一
  • Journal Title: におい・かおり環境学会誌 (印刷中)
  • NAID: 10025515202
• [Journal Article] Development of a Screening System for the Evaluation of Soybean Volatiles. Biosci (2009)
  • Author(s): Kakumyan, P., Kato, M., Hajika, M., Matsui, K.
  • Journal Title: Biotechnol. Biochem (in press)
  • Peer Reviewed
• [Journal Article] Chemical and molecular ecology of herbivore-induced plant volatiles : proximate factors and their ultimate functions (2009)
  • Author(s): Arimura, G, Matsui, K., Takabayashi, J.
  • Journal Title: Plant Cell Physiol 50 Pages: 911-923
  • NAID: 10027339979
  • Peer Reviewed
• [Journal Article] Evidence for protein radical-mediated nuclear tunneling in fatty acid alpha-oxygenase (2008)
  • Author(s): Gupta, A., Mukherjee, A., Matsui, K., Roth, J.P.
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 11274-11275
  • Peer Reviewed
• [Journal Article] Takabayashi, J. : Direct fungicidal activities of C6-aldehydes are important constituents for defense responses in Arabidopsis against Botrytis cinerea (2008)
  • Author(s): Kishimoto, K., Matsui, K., Ozawa, R.
  • Journal Title: Phytochemistry 69 Pages: 2127-2132
  • Peer Reviewed
• [Journal Article] Evidence for protein radical-mediated nuclear tunneling in fatty acid alpha-oxygenase. (2008)
  • Author(s): Gupta A
  • Journal Title: Journal of American Chemical Society 1 3 0 Pages: 11274-11275
  • Peer Reviewed
• [Journal Article] Direct fungicidal activities of C6-aldehydes are important constituents for defence responses in Arabidopsis against Botrytis cinerea (2008)
  • Author(s): Kishimoto K
  • Journal Title: Phytochemistry 69 Pages: 2127-2132
  • Peer Reviewed
• [Journal Article] Identification of an allele attributable to formation of cucumber-like flavor in wild tomato species (Solanum pennellii) that was inactivated during domestication. (2007)
  • Author(s): Matsui, K., Ishii, M., Sasaki, M., Rabinowitch, HD., Ben-Oliel, G.
  • Journal Title: J. Agric. Food Chem. 55 Pages: 4080-4086
  • Peer Reviewed
• [Journal Article] Cloning of lipoxygenase genes from cyanobacterium, Nostoc punctiforme, and its expression in Eschelichia coli (2007)
  • Author(s): Koeduka, T., Kajiwara, T., Matsui, K.
  • Journal Title: Curr. Microbiol 54 Pages: 315-319
  • Peer Reviewed
• [Journal Article] Volatile 1-octen-3-ol induces a defensive response in Arabidopsis thaliana. (2007)
  • Author(s): Kishimoto, K., Matsui, K., Ozawa, R., Takabayashi, J.
  • Journal Title: J Gen. Plant Pathol 73 Pages: 35-37
  • NAID: 10018742530
  • Peer Reviewed
• [Journal Article] みどりの香り-植物が交わす危険信号- (2007)
  • Author(s): 松井健二
  • Journal Title: AROMA RESEARCH 8 Pages: 80-86
• [Journal Article] Identification of an allele attributable to formation of cucumber-like flavor in wild tomato species(Solanum pennellii)that was inactivated during domestication (2007)
  • Author(s): Matsui K.
  • Journal Title: J. Agric. Food Chem. 55 Pages: 4080-4086
  • Peer Reviewed
• [Journal Article] Cloning of lipoxygenase genes from cyanobacterium, Nostoc punctiforme,and its expression in Eschelichia coli (2007)
  • Author(s): Koeduka T.
  • Journal Title: Curr. Microbiol. 54 Pages: 315-319
  • Peer Reviewed
• [Journal Article] みどりの香り-植物が交わす危険信号 (2007)
  • Author(s): 松井 健二
  • Journal Title: AROMA RESEARCH 8 Pages: 80-86
• [Journal Article] Volatile l-octen-3-ol induces a defensive response in Arabidopsis thaliana. (2007)
  • Author(s): Kishimoto, K.
  • Journal Title: Journal of General Plant Pathology 73 Pages: 35-37
• [Journal Article] Green leaf volatiles : hydroperoxide lyase pathway of oxylipin metabolism (2006)
  • Author(s): Matsui, K.
  • Journal Title: Curr. Opin. Plant Biol 9 Pages: 274-280
• [Presentation] みどりの香り放散の化合物特異性 (2009)
  • Author(s): 松井健二
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: マリンメッセ福岡(福岡県)
  • Year and Date: 2009-03-29
• [Presentation] Characterization of volatile compounds in raw and cooked mushroom, Astraes hygrometricus (Chuchi-gri) (2009)
  • Author(s): Pattana Kakumyan
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: マリンメッセ福岡(福岡県)
  • Year and Date: 2009-03-29
• [Presentation] シロイヌナズナのみどりの香り生成経路はジャスモン酸生成経路と発現部位を棲み分けている (2009)
  • Author(s): 松井健二
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2009-03-21
• [Presentation] 疲食害リママメから放出される揮発性化合物が誘導する健全リママメのハダニ抵抗性誘導 (2009)
  • Author(s): 杉本貢一
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2009-03-21
• [Presentation] 植物の傷害応答にみどりの香りはシグナルとして機能する (2008)
  • Author(s): 松井健二
  • Organizer: 第52回香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: 東洋大学(群馬県)
  • Year and Date: 2008-11-25
• [Presentation] Characterization of volatile compounds in canned Puff Ball mushrooms, Astraeus hygrometricus (Pers. ) Morgan, widely consumed in northern Thailand (2008)
  • Author(s): Kakumyan K
  • Organizer: 日本農芸化学会中四国支部第22回講演会
  • Place of Presentation: 鳥取大学(鳥取県)
  • Year and Date: 2008-09-13
• [Presentation] Plant oxylipins formed through P450s and their significance as signaling molecules in plants (2008)
  • Author(s): Matsui K.
  • Organizer: 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: ニース、フランス
  • Year and Date: 2008-06-11
• [Presentation] Plant oxylipins formed through P450s and their significance as signaling molecules in plants (2008)
  • Author(s): Matsui K
  • Organizer: 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: ニース(フランス)
  • Year and Date: 2008-06-11
• [Presentation] 植物による生態系モニタリングでの揮発性化合物の意義 (2008)
  • Author(s): 松井 健二
  • Organizer: 第86回生存圏シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2008-01-29
• [Presentation] 揮発性アルデヒドを介した植物-植物コミュニケーション (2008)
  • Author(s): 松井 健二
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] Are green leaf volatiles involved in warning neighboring cells or plants? Gordon Research Conferences (2007)
  • Author(s): Matsui K.
  • Organizer: Floral & Vegetative Volatiles
  • Place of Presentation: ルディアブレ, スイス
  • Year and Date: 2007-10-07
• [Presentation] Green leaf volatiles : Inter- and intra-plant signal molecules (2007)
  • Author(s): Matsui K.
  • Organizer: The 4th Asia-Pacific Conference on Chemical Ecology, Tsukuba
  • Place of Presentation: つくば
  • Year and Date: 2007-09-12
• [Presentation] Green leaf volatiles:Inter-and intra-plant signal molecules (2007)
  • Author(s): Matsui K
  • Organizer: The 4^<th> Asia-Pacific Conference on Chemical Ecology
  • Place of Presentation: Tsukuba Japan
  • Year and Date: 2007-09-12
• [Presentation] Molecular aspects of plant induced defense against insects and pathogen (2007)
  • Author(s): Matsui K.
  • Organizer: The 23rd International Society of Chemical Ecology Annual Meeting, Jena, Germany
  • Place of Presentation: イェナ、ドイツ
  • Year and Date: 2007-07-24
• [Presentation] Molecular aspects of plant induced defense against insects and pathogen (2007)
  • Author(s): Matsui K
  • Organizer: The 23^<rd> International Society of Chemical Ecology Annual Meeting
  • Place of Presentation: Jena,Germany
  • Year and Date: 2007-07-20
• [Presentation] みどりの香りによる植物の生存戦略 (2007)
  • Author(s): 松井 健二
  • Organizer: 第51回香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: 長浜
• [Presentation] 植物による生態系モニタリングでの揮発性化合物の意義 (2007)
  • Author(s): 松井 健二
  • Organizer: 第81回生存圏シンポジウム
  • Place of Presentation: 京都
• [Presentation] Are green leaf volatiles inter-or intra-plant signal molecules in plants (2007)
  • Author(s): Matsui K
  • Organizer: A Joint Meeting of The Second Asian Symposium on Plant Lipids & The 20^<th> Japanese Symposium on Plant Lipids
  • Place of Presentation: Tokyo,Japan
• [Presentation] Are green leaf volatiles involved in warning neighboring cells or plants (2007)
  • Author(s): Matsui K
  • Organizer: Gordon Research Conferences, Floral&Vegetative Volatiles
  • Place of Presentation: Les Diablerets,Switzerland
• [Presentation] みどりの香りによる防御応答誘導へのグルタチオンの関与 (2006)
  • Author(s): 岸本久太郎, 原志津香, 小澤理香, 高林純示, 松井健二
  • Organizer: 第19回植物脂質シンポジウム
  • Place of Presentation: 佐賀
  • Year and Date: 2006-11-24
• [Book] 種子脂質の分解 種子の科学とバイオテクノロジー(第3章8項)(原田久也監修) (2009)
  • Author(s): 松井健二
  • Publisher: 学会出版センター
• [Book] 種子の科学とバイオテクノロジー (3-8 種子脂質の分解) (2009)
  • Author(s): 松井健二
  • Total Pages: 5
  • Publisher: 学会出版センター
• [Book] Volatile oxylipins and related compounds formed under stress in plants. In Lipidomics. (Armstrong, D. ed.)
  • Author(s): Matsui, K., Sugimoto, K., Kakumyan, P., Khorobrykh, S.A., Mano, J.
  • Publisher: Methods in Molecular Biology, Humana Press Inc. NJ, USA(in press)
• [Remarks]
  • URL: http://web.cc.yamaguchi-u.ac.jp/~matsui/
• [Remarks] 読売新聞2007年5月21日朝刊
• [Remarks] 日本種苗新聞2007年7月21日
• [Remarks] 朝日新聞2008年3月3日朝刊
• [Remarks] 読売新聞2009年4月4日夕刊
• [Remarks]
  • URL: http://web.cc.yamaguchi-u.ac.jp/~matsui/