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Different response of amino acids on muscle protein synthesis and degradation under nutritional stresses

Research Project

Project/Area Number 18580110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionIwate University

Principal Investigator

NAGASAWA Takashi  Iwate University, Faculty of Agriculture, Professor (80189117)

Co-Investigator(Kenkyū-buntansha) ITO Yoshiaki  Iwate University, Faculty of Agriculture, Associate Professor (50312517)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsmuscle / amino acids / leucine / protein synthesis / protein degradation
Research Abstract

1. The function of leucine under low nutritional conditions Skeletal muscle weight decreased in feeding of one week protein free diet, however the weight loss was suppressed in 1.5% leucine supplementation. Muscle protein degradation evaluated by A-V difference of 3-methylhistidine concentration was also suppressed by leucine supplemented diet. The rate of muscle protein synthesis was not changed. Although ubiquitin-proteasome expression and calpain activity were not reduced in feeding of leucine supplemented diet, LC-3II expression was decreased. These results suggested that leucine enhances muscle protein degradation rather synthesis and the degradation might be regulated by autophagy in low nutritional conditions.
2. Effects of repeated leucine consumption on muscle protein synthesis and degrdation Six hours interval leucine administration to rats decreased myofibrillar protein degradation evaluated by 3-methylhistidine release from the isolated muscle. However, synthesis evaluated by phosphrylation of S6K1 did not, suggesting that repeated leucine supplementation is beneficial for muscle atrophy.
3. Effects of leucine on muscle atrophy in disuse model Immobilization of hind limb increased muscle protein degradation and then resulted muscle atrophy. In this model, leucine supplemented diet attenuated muscle atrophy due to muscle protein degradation especially ubiquitin-proteasome.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (21 results)

All 2008 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (15 results)

  • [Journal Article] Supplementation with dietary leucine to a protein-deficient diet suppresses myofib rillar protein degradation in rats2008

    • Author(s)
      Sugawara, T., Ito, Y., Nishizawa, N, Nagasawa, T
    • Journal Title

      J. Nutr. Sci. Vitaminol.(Tokyo) 53

      Pages: 525-555

    • NAID

      110006531878

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Supplementation with Dietary Leucine to a Protein-Deficient Diet Suppresses Myofibrillar Protein Degradation in Rats2007

    • Author(s)
      Sugawara T, et. al.
    • Journal Title

      J. Nutr. Sci. Vitaminol. 53

      Pages: 552-555

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 代謝調節因子として注目される分岐鎖アミノ酸2007

    • Author(s)
      吉澤史昭、長澤孝志
    • Journal Title

      化学と生物 45

      Pages: 203-210

    • NAID

      10018572959

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Supplementation with Dietary Leucine to a Protein-Deficient Diet Suppresses Myofibrillar Protein Degradation in Rats2007

    • Author(s)
      Sugawara T, et. al.
    • Journal Title

      J. Nutr. Sci. Vitaminol Vol. 53

      Pages: 552-555

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Imoportance of Branched-chain Amino Acids As Regulatory Factors of Metabolism2007

    • Author(s)
      Yoshizawa, F, and Nagasawa, T.
    • Journal Title

      Kagaku to Seibuts Vol 45

      Pages: 203-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 代謝調節因子として注目される分岐鎖アミノ酸2007

    • Author(s)
      吉澤史昭, 長澤孝志
    • Journal Title

      化学と生物 45巻3号

      Pages: 203-210

    • NAID

      10018572959

    • Related Report
      2006 Annual Research Report
  • [Presentation] ロイシン投与による骨格筋萎縮抑制における筋タンパク質分解の役割2007

    • Author(s)
      菅原貴征, ら
    • Organizer
      第1回日本アミノ酸学会学術講演会
    • Place of Presentation
      東京大学
    • Year and Date
      2007-11-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The Role of Muscle Protein Degradation in Skeletal Muscle Atrophy Administered Leucine2007

    • Author(s)
      Sugawara T, ed. al.
    • Organizer
      1^<st> Annual Meetings of Japan Society of Amino Acid Sciences
    • Place of Presentation
      The University of Tokyo
    • Year and Date
      2007-11-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Leucine attenuates myofibrillar protein degradation of rats fed on protein deficient diet2007

    • Author(s)
      Sugawara T , et. al.
    • Organizer
      10th Asian Congress of Nutrition
    • Place of Presentation
      台北国際会議場(台湾)
    • Year and Date
      2007-09-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Leucine Attenuates Myofibrilalr Protein Degradation of Rats Fed on Protein Free Diet2007

    • Author(s)
      Sugawara T, ed. al.
    • Organizer
      10^<th> Asian Congress of Nutrition
    • Place of Presentation
      Taipei
    • Year and Date
      2007-09-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Leucine attenuates myofibrillar protein degradation of rats fed on protein deficient diet2007

    • Author(s)
      Sugawara, T., Ito, Y., Nishizawa, N, Nagasawa, T
    • Organizer
      10th Asian Congress of Nutrition (第10回アジア栄養学会議)
    • Place of Presentation
      台湾、台北市、台北国際会議場
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] 後肢固定による筋萎縮における酸化ストレスとロイシン、カテキンの効果2007

    • Author(s)
      長澤孝志, ら
    • Organizer
      第15回生体パーオキサイド研究会
    • Place of Presentation
      勝山館(仙台市)
    • Year and Date
      2007-08-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of Leucine and Catechin on Oxidative Stress and Muscle Atrophy by Hind Limb Fixing2007

    • Author(s)
      Nagasawa T, ed. al.
    • Organizer
      The 15^<th> Sendai Conference of Lipid Peroxide Biology and Medicine
    • Place of Presentation
      Shozankan
    • Year and Date
      2007-08-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ロイシン、タンパク質の摂食による筋タンパク質の合成と分解の変化2007

    • Author(s)
      菅原貴征, ら
    • Organizer
      第61回日本栄養・食糧学会大会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2007-05-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Changes in Muscle Protein Synthesis and Degradation by Dietary Leucine and Protein2007

    • Author(s)
      Sugawara T, ed. al.
    • Organizer
      61^<th> Annual Meetings of Japanese Society of Nutrition and Food Sciences
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2007-05-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 複数回のロイシン投与と骨格筋タンパク質の合成、分解2007

    • Author(s)
      井口愼平, ら
    • Organizer
      2007年度日本農芸化学会大会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2007-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Multiple Administration of Leucine and Protein Synthesis and Degradation of Skeletal Muscle2007

    • Author(s)
      Iguchi S, ed. al.
    • Organizer
      2007 Annual Meetings of Japan Society of Bioscience, Biotechnology and Agro-chemistry
    • Place of Presentation
      Tokyo University of Agriculture
    • Year and Date
      2007-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 摂食タイミングによる骨格筋タンパク質の分解と合成の調節2006

    • Author(s)
      井口愼平, ら
    • Organizer
      平成18年度日本農芸化学会北海道支部・東北支部合同支部大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2006-11-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of Muscle Protein Synthesis and Degradation by Feeding Timing2006

    • Author(s)
      Iguchi S, ed. al.
    • Organizer
      Annual Meetings of Japan Society of Bioscience, Biotechnology and Agro-chemistry, Tohoku Branch
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2006-11-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 無タンパク質食の摂取は筋原線維タンパク質の分解を促進し、Leuはそれを阻害する2006

    • Author(s)
      菅原貴征, ら
    • Organizer
      第60回日本栄養・食糧学会大会
    • Place of Presentation
      静岡県立大学
    • Year and Date
      2006-05-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Protein Free diet Stimulates Myofibrillar Protein Degradation and Leucine Supporesses It2006

    • Author(s)
      Sugawara T, ed. al.
    • Organizer
      60^<th> Annual Meetings of Japanese Society of Nutrition and Food Sciences
    • Place of Presentation
      The University of Shizuoka
    • Year and Date
      2006-05-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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