| Project/Area Number |
18580113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAI Yuji The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor (10321788)
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| Co-Investigator(Kenkyū-buntansha) |
HISATSUNE Tatsuhiro The University of Tokyo, Graduate School of Frontier Sciences, Associate Professor (10238298)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | food / physiology / proteomics / receptor / polyphenol |
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| Research Abstract |
From preliminary investigation, we found a vasodilatation effect of (+)-catechin on the endothrial cells. The effect was observed within 30 minutes after (+)-catechin treatment in vivo, but not (-)-epigallocatechin gallate, we hypothesized that the existence of novel (+)-catechin specific receptor and that the effect was mediated through the receptor signaling thereby produced NO. In order to identify putative (+)-catechin receptor, we used a differential proteomics approach in the brain of (+)-catechin injected mice. Male, 8-week-old ICR mice were injected 100 mg/ml (+)-catechin intravenously. After 3h injection, the brain was excised and homogenized to extract proteins. The brain extracts were subjected to 2-D PAGE, and then the 2-D gels were silver stained. The spots with a pI of 〜8.1 and a molecular mass of 〜60 kDa and a pI of 〜8.3 and a molecular mass of 〜17 kDa were markedly increased in (+)-catechin injected mouse brain extract compared with vehicle injected control. While the spot with a pI of 〜5.7 and a molecular mass of 22 kDa was decreased. These changes in spots are suspected to be brought about by the post-translational modification such as protein phosphorylation or protein degradation of the putative receptor itself or its downstream signaling molecules. Next, we will try to isolate these protein spots and analyze the amino acid sequences using peptide mass fingerprinting.
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