Development of anti-Parkinson's disease functional foods with enhancements of DNA repair and protein quality control
| Project/Area Number |
18580123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kyushu University |
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Principal Investigator |
TERUYA Kiichiro Kyushu University, Faculty of Agriculture, Assistant Professor (10273971)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | cell, tissue / foods / cranial nerve disorders / stress / signal transduction |
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| Research Abstract |
Fermented milk Kefir, enzyme-digested fucoidan extracts and electrorized reduced water were investigated as anti-Parkinson's disease functions. To evaluate suppression of cytotoxicity induced nitric oxide (NO) donor, human dopaminergic neuron model SH-SY5Y cells were treated with SIN-1 or sodium nitroprusside. The cytotoxicity was determined by WST-1 assay. An apoptosis was evaluated by immunoblotting about caspase activations. However, candidate functional foods and waters were not effective for protection of dopaminergic neurons against NO donor.
To evaluate activation for protein quality control system in SH-SY5Y cells were treated with potent proteasome inhibitor MG-132. The treatment of enzyme-digested fucoidan extracts suppressed the ubiquitinated protein accumulation in the cells, and increased the proteasome activities.
The enzyme-digested fucoidan extracts were orally administrated Parkinson's disease model mouse (MPTP and probenecid administrated C57BL/6J male mice). It was observed that depression of spontaneous movement was suppressed and tyrosine hydroxylase expression in mid brain was protected against MPTP damages. Since, tyrosine hydroxylase is the key enzyme of dopamine synthesis, it was suggested that the enzyme-digested fucoidan extract had the protective effect for dopaminergic neurons against MPTP damages.
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Report
(3 results)
Research Products
(53 results)
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[Presentation] NKGケフィアのエネルギー代謝充進効果2007
Author(s)
小木 貴博, ら
Organizer
第61回日本栄養・食糧学会九州・沖縄支部大会
Place of Presentation
尚絅大学(熊本県熊本市)
Year and Date
2007-09-29
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] NKG Kefir upregulates energy metabolism2007
Author(s)
Ogi, T., et. al.
Organizer
61st Meetings of Kyushu & Okinawa Branch, Japanese Society of Nutrition and Food Science
Place of Presentation
Shokei Univ, (Kumamoto, Japan)
Year and Date
2007-09-29
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Suppression of adipogenesis by NKG Kefir2007
Author(s)
Teruya, K., et. al.
Organizer
Annual Meetings of Japan Society for Bioscience, Biotechnology, and Agrochemistry 2007
Place of Presentation
Tokyo Univ, of Agriculture(Tokyo, Japan)
Year and Date
2007-03-25
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Anti-type 2 diabetes mellitus agents in NKG Kefir2006
Author(s)
Sakai, K., et. al.
Organizer
Meetings of Kyushu & Okinawa Branch, Japanese Society of Nutrition and Food Science, and Western-Japan Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Saga Univ, (Saga, Japan)
Year and Date
2006-09-30
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Suppressive effect of adipogenesis by NKG Kefir2006
Author(s)
Toriyama, H., et. al.
Organizer
Meetings of Kyushu & Okinawa Branch, Japanese Society of Nutrition and Food Science, and Western-Japan Branch, Japan Society for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Saga Univ, (Saga, Japan)
Year and Date
2006-09-30
Description
「研究成果報告書概要(欧文)」より
Related Report
