Signaling pathways for the inhibition of cell transformation by anthocyanins

• Project/Area Number: 18580125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kagoshima University
• Principal Investigator: HOU De-xing Kagoshima University, 農学部, 准教授 (90305160)
• Co-Investigator(Kenkyū-buntansha): FUJII Makoto 鹿児島大学, 農学部, 教授 (70041655) ; HASHIMOTO Fumio 鹿児島大学, 農学部, 准教授 (70244142)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,970,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: アントシアニン / 細胞がん化 / シグナル伝達 / 標的分子 / 分子機構 / 細胞癌化抑制能 / 分子標的 / MEK1 / 食品分子作用機構 / 細胞シグナル伝達 / NF-kB / 分子作用機構 / 食品機能性

Research Abstract

アントシアニンは抗酸化作用を始め多くの生体調節機能が報告されているが、その分子作用機構には不明の点が多い。本研究は分子生物学的手法および遺伝子網羅的な解析手法を用いてアントシアニンによる細胞癌化の信号伝達経路、標的分子および下流の遺伝子の発現制御を解析した。その結果、アントシアニンが少なくともプロティンキナーゼMEK1と直接的に結合し、MAPKの伝達経路を抑制し、細胞炎症・癌化を制御することが明らかとなった。

Research Products

• [Journal Article] Myricetin directly targets JAK1 to inhibit cell transformation (2009)
  • Author(s): Kumamoto T, Fujii M, Hou DX*
  • Journal Title: Cancer Lett. 275 Pages: 17-26
  • Peer Reviewed
• [Journal Article] Expression profiling of genes targeted by bilberry(Vaccinium myrtillus)in macrophages through DNA microarray (2008)
  • Author(s): Chen J, Uto T, Tanigawa S, Kumamoto T,Fujii M, Hou DX*
  • Journal Title: Nutr. Cancer. 60 Pages: 43-50
  • Peer Reviewed
• [Journal Article] Molecular mechanism of apoptosis induced by schizandrae-derived lignans in human leukemia HL-60 cells (2008)
  • Author(s): Lin S, Fujii M, Hou DX*
  • Journal Title: Food Chem. Toxicol. 46(2) Pages: 590-597
  • Peer Reviewed
• [Journal Article] Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells (2008)
  • Author(s): Tanigawa S, Fujii M, Hou DX*
  • Journal Title: Biosci. Biotechnol. Biochem. 72(3) Pages: 797-804
  • NAID: 10027525776
  • Peer Reviewed
• [Journal Article] Expression profiling of genes targeted by bilberry (Vaccinium myrtillus) in macrophages through DNA microarray. 60:43-50(2008) (2008)
  • Author(s): Chen J, Uto T, Tanigawa S, Kumamoto T, Fujii M, Hou DX*
  • Journal Title: Nutrition and Cancer 60 Pages: 43-50
  • Peer Reviewed
• [Journal Article] Molecular mechanism of apoptosis induced by schizandrae-derived lignans in human leukemia HL-60 cells. (2008)
  • Author(s): Lin S, Fujii M, Hou DX.
  • Journal Title: Food Chem Toxicol. 46(2) Pages: 590-507
  • Peer Reviewed
• [Journal Article] Green tea proanthocyanidins inhibit cyclooxygenase-2 expression in LPSactivated mouse macrophages : molecular mechanisms and structure-activity relationship (2007)
  • Author(s): Hou DX*, Masuzaki S, Hashimoto F, UtoT, Tanigawa S, Fujii M, Sakata Y
  • Journal Title: Arch. Biochem. Biophys. 460(1) Pages: 67-74
  • Peer Reviewed
• [Journal Article] Prodelphinidin B-4 3'-O-gallate, a tea polyphenol, is involved in the inhibition of COX-2 and iNOS via the downregulation of TAK1-NF-kB pathway (2007)
  • Author(s): Hou DX*, Dong Luo, Tanikawa S, HashimotoF, Uto T, Masuzaki S, Fujii M, SakataY
  • Journal Title: Biochem. Pyarm. 74 Pages: 742-752
  • Peer Reviewed
• [Journal Article] Action of Nrf2 and Keap1 in ARE-mediated NQO1 expression by quercetin (2007)
  • Author(s): Tanigawa S, Fujii M, Hou DX*
  • Journal Title: Free Radic.Biol. Med. 42(11) Pages: 1690-1703
  • Peer Reviewed
• [Journal Article] Effects of 6-(methylsulfinyl)hexylisothiocyanat e on cyclooxygenase-2 expression nduced by lipopolysaccharide, interferon-gamma and 12-O-tetradecanoylphorbol-13-acetate (2007)
  • Author(s): Uto T, Fujii M, Hou DX*
  • Journal Title: Oncol Rep. 17 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Prodelphinidin B-4 3'-O-gallate, a tea polyphenol, is involved in the inhibition of COX-2 and iNOS via the downregulation of TAK1-NF-kappaB pathway. (2007)
  • Author(s): Hou DX, Luo D, Tanigawa S, Hashimoto F, Uto T, Masuzaki S, Fujii M, Sakata Y.
  • Journal Title: Biochem Pharmacol. 74(5) Pages: 742-751
  • Peer Reviewed
• [Journal Article] Green tea proanthocyanidins inhibit cyclooxygenase-2 expression in LPS-activated mouse macrophages : Molecular mechanisms and structure-activity relationship. (2007)
  • Author(s): Hou DX, MasuzakiS, Hashimoto F, Uto T, Tanigawa S, Fujii M, Sakata Y.
  • Journal Title: Archives of Biochemistry and Biophysics 460 Pages: 67-74
• [Journal Article] Effects of 6-(methylsulfinyl)hexyl isothiocyanate on cyclooxygenase-2 expression induced by lipopolysaccharide, interferon-gamma and 12-O-tetradecanoylphorbol-13-acetate. (2007)
  • Author(s): Uto T, Fujii M, Hou DX.
  • Journal Title: Oncology Reports 17(1) Pages: 233-238
• [Journal Article] ビルベリーのアントシアニンと機能性 (2006)
  • Author(s): 侯徳興
  • Journal Title: FOOD Style 21 10(8) Pages: 13-17
• [Journal Article] Action of Nrf2 and Keap1 in ARE-mediated NQO1 expression by quercetin
  • Author(s): Tanigawa S, Fujii M, Hou DX.
  • Journal Title: Free Radical Biology and Medicine (印刷中)
• [Presentation] MEK1を分子標的としたDp-3-Samによる細胞形質転換の抑制 (2009)
  • Author(s): 十合貴之、隈元拓馬、寺原典彦、藤井信、侯徳興
  • Organizer: 日本農芸化学会2009年大会シンポジウム
  • Place of Presentation: 福岡
• [Presentation] プロテインキナーゼを分子標的とするポリフェノールの機能性展開 (2009)
  • Author(s): 侯 徳興
  • Organizer: 日本農芸化学会2009年度大会シンポジウム : フードケミカルバイオロジー
  • Place of Presentation: 福岡国際センター
• [Presentation] MEK1を分子標的としたハイビスカスDp-3-Samによる細胞形質転換の抑制 (2009)
  • Author(s): 十合 貴之, 隈元 拓馬, 寺原典彦, 藤井 信, 侯 徳興
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際センター
• [Presentation] DNAマイクアレーを用いたアントシアニンの抗炎症作用の網羅的な解析 (2008)
  • Author(s): 陳継華、藤井信、侯徳興
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 埼玉
• [Presentation] DNAマイクアレーを用いたアントシアニンの抗炎症作用の網羅的な解析 (2008)
  • Author(s): 陳 継華, 藤井 信, 侯 徳興
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 日本栄養女子大学
• [Presentation] ブルーベリー機能性研究の最新事情 (2007)
  • Author(s): 侯徳興
  • Organizer: 第6回ブルーベリー国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-11-29
• [Presentation] Bilberry anthocyanins andbiological activities (2007)
  • Author(s): HOU DX
  • Organizer: 3rd International Conference on Polyphenols and Health(ICPH2007)
  • Place of Presentation: シンポジウム、京都
• [Presentation] Anthocyanins and Health : Chemopreventive Activities and Molecular Targets (2007)
  • Author(s): Hou DX
  • Organizer: The Satellite S ymposium of ICPH2007
  • Place of Presentation: シンポジウム、鹿児島
• [Presentation] Anthocyanins-more than nature color, chemopreventive activities and molecular targets (2007)
  • Author(s): Hou DX
  • Organizer: 10th World Congress on Advances in Oncology
  • Place of Presentation: シンポジウム、クレタ島、ギリシャ
• [Presentation] ブルーベリーアントシアニンの組成と機能性の関係について (2007)
  • Author(s): 佐賀聡美、侯徳興、山本雅史、藤井信、寺原典彦
  • Organizer: 日本農芸化学会2007年大会
  • Place of Presentation: 東京
• [Presentation] American Chemical Society 232nd National Meeting : Anthocyans and human health : evidence from cellular (2006)
  • Author(s): HOU DX
  • Organizer: investigations
  • Place of Presentation: シンポジウム、サンフランシスコ, 米国
  • Year and Date: 2006-09-11
• [Book] Chapter 24. Cancer-preventive effects and molecular actions of anthocyanins in Nutrigenomics and Proteomics in Health and Disease : Food Factors and Gene Interactions (2009)
  • Author(s): HHou DX
  • Publisher: Wiley Publishers
• [Book] Nutrigenomics and Proteomics in Health and Disease : Food Factors and Gene Interactions (Chapter 24. Cancer-preventive effects and molecular actions of anthocyanins) (2009)
  • Author(s): Hou De-Xing
  • Publisher: WILEY-BLACKWELL
• [Book] Chapter 25. Anthocyans and Chemoprevention : Evidence from Cellular Investigations in Functional Food and Health. (2008)
  • Author(s): Hou DX
  • Publisher: American Chemistry Society
• [Book] Chapter 5, Chemopreventive activities and molecular targets of anthocyanins in Anthocyanins as Food Factor : Recent Progress in Studies on Bioavailability and Health Promotion Effects (2008)
  • Author(s): Hou DX
  • Publisher: Research Signpost Press
• [Book] Functional Food and Health (Chapter 25. Anthocyans and Chemoprevention : Evidence from Cellular Investigations). (2008)
  • Author(s): Hou De-Xing
  • Publisher: American Chemistry Society
• [Book] Anthocyanins as Food Factors : Recent Progress in Studies on Bio-availability and Health Promotion Effects. (2008)
  • Author(s): Hou De-xing
  • Publisher: WILEY-BLACKWELL