Studies on a soluble lectin involved in intestinal mucosal innate immunity

• Project/Area Number: 18580131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Meiji University
• Principal Investigator: WATANABE Hirohito Meiji University, 農学部, 准教授 (20270895)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,070,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 腸管上皮 / 感染防御 / 自然免疫 / レクチン

Research Abstract

腸管上皮の自然免疫機能に関与しうる分子として見出したRegIVの機能を明らかにすることを目的として研究を行った。その結果、RegIVがマンナンなどの糖鎖に結合すること、さらにグラム陽性菌・陰性菌および真菌に対し、その増殖を抑制する作用を有することを見いだした。これらのことからRegIVは腸管上皮より分泌されるレクチンであり、幅広い微生物に対して抗菌作用を示して、感染防御や腸内フローラ制御に重要な役割を担っていることが示唆された。

Research Products

• [Journal Article] Biochemical and genomic analysis of neoculin compared to monocot mannose-binding lectins. (2008)
  • Author(s): Akiko Shimizu-Ibuka, Yuji Nakai, Keisuke Nakamori, Yuji Morita, Ken-ichiro Nakajima, Koji Kadota, Hirohito Watanabe, Satoshi Okubo, Tohru Terada, Tomiko Asakura, Takumi Misaka, and Keiko Abe
  • Journal Title: J. Agric. Food Chem. 56 Pages: 5338-5344
• [Journal Article] Formation mechanisms of melanoidins and fluorescent pyridinium compounds as advanced glycation end products (2008)
  • Author(s): F. Hayase, T. Usui, Y. Ono, Y. Shirahashi, T. Machida, T. Ito, K. Shimohira, H. Watanabe
  • Journal Title: Annals of New York Academy of Science 1126 Pages: 53-58
  • Peer Reviewed
• [Journal Article] Biochemical and genomic analysis of neoculin compared to monocot mannose-binding lectins (2008)
  • Author(s): A. Shimizu-Ibuka, Y. Nakai, K. Nakamori, Y. Morita, K. Kadota, H. Watanabe, S. Okubo, T. Terada, T. Asakura, T. Misaka, K. Abe
  • Journal Title: Journal of Agricultural and Food Chemistry 56 Pages: 5338-5344
  • Peer Reviewed
• [Journal Article] The formation of argpyrimidine in glyceraldehydes-related glycation (2008)
  • Author(s): T.Usui, M.Ohguchi, H.Watanabe, and F.Hayase
  • Journal Title: Biosci Biotechnol Biochem 72 Pages: 568-571
  • NAID: 10027524765
  • Peer Reviewed
• [Journal Article] Determination of glyceraldehyde fbrmed in glucose degradation and glycation (2007)
  • Author(s): T.Usui, M.Yoshino, H.Watanabe, and F.Hayase
  • Journal Title: Biosci Biotechnol Biochem 71 Pages: 2162-2168
  • Peer Reviewed
• [Journal Article] Identification and determination of alpha-dicarbonyl compounds formed in the degradation of sugars (2007)
  • Author(s): T.Usui, S.Yanagisawa, M.Ohguchi, M.Yoshino, R.Kawabata, J.Kishimoto, Y.Arai, K.Aida, H.Watanabe, and F.Hayase
  • Journal Title: Biosci Biotechnol Biochem 71 Pages: 2465-2472
  • Peer Reviewed
• [Journal Article] Detection and determination of glyceraldehyde-derived pyridinium-type advanced glycation end product in streptozotocin-induced diabetic rats (2007)
  • Author(s): T.Usui, K.Shimohira, H.Watanabe, F.Hayase
  • Journal Title: Biosci Biotechnol Biochem. 71 Pages: 442-448
  • NAID: 10027511564
• [Journal Article] Isolation and identification of 5-methyl-imidazolin-4-one derivative as glyceraldehyde-derived advanced glycation end product (2006)
  • Author(s): T.Usui, H.Watanabe, F.Hayase
  • Journal Title: Biosci Biotechnol Biochem. 70 Pages: 1496-1498
  • NAID: 10018530468
• [Journal Article] Non-involvement of the human monocarboxylic acid transporter 1(MCT1) in the transport of phenolic acid (2006)
  • Author(s): H.Watanabe, T.Yashiro, Y.Tohjo, Y.Konishi
  • Journal Title: Biosci Biotechnol Biochem. 70 Pages: 1928-1933
  • NAID: 10018528236
• [Journal Article] Identification of a novel blue pigment as a melanoidin intermediate in the D-xylose-glycine reaction system (2006)
  • Author(s): S.Sasaki, Y.Shirahashi, K.Nishiyama, H.Watanabe, F.Hayase
  • Journal Title: Biosci Biotechnol Biochem. 70 Pages: 2529-2531
  • NAID: 10018526109
• [Journal Article] Chemistry and some biological effects of model melanoidins and pigments as Maillard intermediates (2006)
  • Author(s): F.Hayase, T.Usui, H.Watanabe
  • Journal Title: Mol Nutr Food Res. 50 Pages: 1171-1179
• [Presentation] 腸管上皮細胞から分泌されるC-typeレクチンRegIVの機能解析 (2009)
  • Author(s): 高橋玄、岡絵美子、浜本牧子、渡辺寛人
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-03-28