Screening of renin inhibitory activity in Foods and its functional analysis.
Project/Area Number |
18580134
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Food science
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Research Institution | Akita Prefectural Agriculture, Forestry and Fisheries Research Center |
Principal Investigator |
TAKAHASHI Saori Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Akita Research Institute for Food and Brewing, Chief Researcher (10142184)
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Co-Investigator(Kenkyū-buntansha) |
HORI Kazuyuki Akita Prefectural Agriculture, Forestry, and Fisheries Research Center, Akita Research Institute for Food and Brewing, Senior Researcher (50181516)
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Project Period (FY) |
2006 – 2007
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Project Status |
Completed (Fiscal Year 2007)
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Budget Amount *help |
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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Keywords | renin / renin-angiotensin system / synthetic substrate / inhibitor / insect cells / expression / soybean / legumes / アスパルティックプロテアーゼ / 結合タンパク質 / エピメラーゼ / ヌクレオチド / ATP |
Research Abstract |
The renin-angiotensin-aldosterone system (RAS) plays an essential role in blood pressure control in animals. Renin catalyzes the liberation of angiotensin I from plasma substrate angiotensinogen. The produced decapeptide angiotensin I is an inactive peptide and activated by angiotensin converting enzyme (ACE). ACE cleaves C-terminal dipeptide from angiotensin I. The produced angiotensin II raises blood pressure by vasoconstriction as well as stimulation of the synthesis and release of aldosterone. Thus, renin is a key enzyme of RAS. ACE has been used as a target enzyme in RAS for screening inhibitors because of its simple assay method; however, renin is a rate-limiting enzyme in RAS, so it was not used because the measurement is very complicated. In the present study, we expressed recombinant human renin in Escherichia coil cells, refolded and activated by trypsin. Using recombinant human renin as a target enzyme, we screened the renin inhibitory activity in fermented soybean paste (miso) and found that soybean, the major ingredient of miso, had renin inhibitory activity. Moreover, we expressed recombinant human renin in insect Sf-9 cells and found that mature active renin accumulated in the late stage of cultivation. Using the medium, we simply purified recombinant human renin and characterized various properties of it. Our method should provide a large quantity of recombinant human renin using the baculovirus expression system.
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Report
(3 results)
Research Products
(45 results)
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[Presentation] 雑豆類由来レニン阻害物質について2008
Author(s)
高橋砂織、堀 一之, 他2名
Organizer
2008年度日本農芸化学会大会
Place of Presentation
名城大学(名古屋市)
Year and Date
2008-03-28
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Renin inhibitory activity in beans.(Japanese)2008
Author(s)
Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
Organizer
2008 Annual Meeting of Japan Societry for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Meijo University(Nagogy)
Year and Date
2008-03-28
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Renin inhibitor in legumes.2007
Author(s)
Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
Organizer
ICOFF2007, International Conference on Food Factors for Health Promotion
Place of Presentation
Kyoto International Conference Hall(Kyoto)
Year and Date
2007-11-30
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Mature human renin expressed in insect cells.2007
Author(s)
Saori Takahashi, Keishi Hata, Ken-Ichi Kikuchi, Takeshi Gotho
Organizer
5th General Meeting of the International Proteolysis Society
Place of Presentation
University of Patras, (Patras, Greece)
Year and Date
2007-10-23
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] 食物由来レニン阻害物質について2007
Author(s)
高橋砂織, 他6名
Organizer
食品化学工学会54回大会
Place of Presentation
中村学園大学(福岡市)
Year and Date
2007-09-08
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Renin inhibitor from foods.(Japanese)2007
Author(s)
Saori Takahashi, Takayuki Watanabe, Hironobu Ogasawara, Masanori Kumagai, Keishi Hata, Kazuyuki Hori, Mari Sugawara
Organizer
54th Annual Meeting of Japanese Society for Food Science and Technlogy
Place of Presentation
Nakamura-Gakuen University(Fukuoka)
Year and Date
2007-09-08
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Renin inhibitor form miso and soybean.(Japanese)2007
Author(s)
Saori Takahashi, Hironobu Ogasawara, Takayuki Watanabe, Masanori Kumagai, Kaoru Owari, Kazuyuki Hori
Organizer
2007 Annual Meeting of Japan Society for Bioscience, Biotechnology and Agrochemistry
Place of Presentation
Tokyo University of Agriculture(Tokyo)
Year and Date
2007-03-25
Description
「研究成果報告書概要(欧文)」より
Related Report
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