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Screening of renin inhibitory activity in Foods and its functional analysis.

Research Project

Project/Area Number 18580134
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionAkita Prefectural Agriculture, Forestry and Fisheries Research Center

Principal Investigator

TAKAHASHI Saori  Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Akita Research Institute for Food and Brewing, Chief Researcher (10142184)

Co-Investigator(Kenkyū-buntansha) HORI Kazuyuki  Akita Prefectural Agriculture, Forestry, and Fisheries Research Center, Akita Research Institute for Food and Brewing, Senior Researcher (50181516)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsrenin / renin-angiotensin system / synthetic substrate / inhibitor / insect cells / expression / soybean / legumes / アスパルティックプロテアーゼ / 結合タンパク質 / エピメラーゼ / ヌクレオチド / ATP
Research Abstract

The renin-angiotensin-aldosterone system (RAS) plays an essential role in blood pressure control in animals. Renin catalyzes the liberation of angiotensin I from plasma substrate angiotensinogen. The produced decapeptide angiotensin I is an inactive peptide and activated by angiotensin converting enzyme (ACE). ACE cleaves C-terminal dipeptide from angiotensin I. The produced angiotensin II raises blood pressure by vasoconstriction as well as stimulation of the synthesis and release of aldosterone. Thus, renin is a key enzyme of RAS. ACE has been used as a target enzyme in RAS for screening inhibitors because of its simple assay method; however, renin is a rate-limiting enzyme in RAS, so it was not used because the measurement is very complicated. In the present study, we expressed recombinant human renin in Escherichia coil cells, refolded and activated by trypsin. Using recombinant human renin as a target enzyme, we screened the renin inhibitory activity in fermented soybean paste (miso) and found that soybean, the major ingredient of miso, had renin inhibitory activity. Moreover, we expressed recombinant human renin in insect Sf-9 cells and found that mature active renin accumulated in the late stage of cultivation. Using the medium, we simply purified recombinant human renin and characterized various properties of it. Our method should provide a large quantity of recombinant human renin using the baculovirus expression system.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (45 results)

All 2008 2007 2006 Other

All Journal Article (15 results) (of which Peer Reviewed: 6 results) Presentation (27 results) Book (1 results) Remarks (2 results)

  • [Journal Article] N-アセチルグルコサミン 2-エピメラーゼの新規活性測定方法-キチナーゼの活性測定にも応用可能か-2008

    • Author(s)
      高橋砂織、堀 一之
    • Journal Title

      キチン・キトサン研究 14

      Pages: 13-17

    • NAID

      10024329957

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Novel assay method for N-acetyl-glucosamine 2-epimerase : The method can apply chitinase assay.(Japanese)2008

    • Author(s)
      Saori Takahashi, Kazuyuki Hori
    • Journal Title

      Chitin and Chitosan Research 14(1)

      Pages: 13-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] N-アセチルグルコサミン2-エピメラーゼの新規活性測定方法-キチナーゼの活性測定にも応用可能か-2008

    • Author(s)
      高橋 砂織、堀 一之
    • Journal Title

      キチン・キトサン研究 14

      Pages: 13-17

    • NAID

      10024329957

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Human renin inhibitor in legumes.2007

    • Author(s)
      Saori Takahashi, et. al.
    • Journal Title

      J.Biol.Macromol. 7

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] High-level expression of recombinant human renin in Sf-9 cells:Rapid purification and characterization.2007

    • Author(s)
      Saori Takahashi, et. al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 71

      Pages: 2610-2613

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Human renin inhibitor in legumes.2007

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
    • Journal Title

      J. Biol. Macromol. 7(3)

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] High-level expression of recombinant active human renin in Sf-9 cells : Rapid purification and characterization.2007

    • Author(s)
      Saori Takahashi, Keishi Hata, Ken-Ichi Kikuchi, Takeshi Gotho
    • Journal Title

      Biosci. Biotecnol. Biochem. 71(10)

      Pages: 2610-2613

    • NAID

      40016199745

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Human renin inhibitory activity in legumes2007

    • Author(s)
      Saori Takahashi, et. al.
    • Journal Title

      J. Biol. Macromol. 7

      Pages: 49-54

    • NAID

      40016199744

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-level expression of recombinant active human renin in Sf-9 cells:Rapid purification and characterization2007

    • Author(s)
      Saori Takahashi, et. al.
    • Journal Title

      Biosci. Biotechnol. Biochem. 71

      Pages: 2610-2613

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of nucleotides on the interaction of renin with GlcNAc 2-epimerase(Renin binding protein).2006

    • Author(s)
      Saori Takahashi, et. al.
    • Journal Title

      J.Biochem 140

      Pages: 125-730

    • NAID

      10018166918

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Refolding and activation of human renin expressed in Escherichia coli:Application of recombinant human renin for inhibitor screening.2006

    • Author(s)
      Saori Takahashi, et. al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 70

      Pages: 2913-2918

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of nucleotides on the interaction of renin with GlcNAc 2-epimerase(Renin binding protein).2006

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Hironobu Ogasawara, Kazuyuki Hiwatashi, Toshihiro Sugiyama
    • Journal Title

      J. Biochem 140(5)

      Pages: 725-730

    • NAID

      10018166918

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Refolding and activation of human renin expressed in Escherichia coli : Application of recombinant human renin for inhibitor screening.2006

    • Author(s)
      Saori Takahashi, Hironobu Ogasawara, Takayuki Watanabe, Masanori Kumagai, Hiroyasu Inoue, Kazuyuki Hori
    • Journal Title

      Biosci. Biotecnol. Biochem. 70(12)

      Pages: 2913-2918

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of nucleotides on the interaction of renin with GlcNAc2-epimerase (Renin binding protein).2006

    • Author(s)
      Saori Takahashi et al.
    • Journal Title

      Journal of Biochemistry 140(5)

      Pages: 725-730

    • NAID

      10018166918

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Refolding and activation of human prorenin expressed in Escherichia coli : Application of recombinant human renin for inhibitor screening.2006

    • Author(s)
      Saori Takahashi et al.
    • Journal Title

      Bioscience Biotechnology and Biochemistry 70(12)

      Pages: 2913-2918

    • NAID

      40015602171

    • Related Report
      2006 Annual Research Report
  • [Presentation] 雑豆類由来レニン阻害物質について2008

    • Author(s)
      高橋砂織、堀 一之, 他2名
    • Organizer
      2008年度日本農芸化学会大会
    • Place of Presentation
      名城大学(名古屋市)
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Renin inhibitory activity in beans.(Japanese)2008

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
    • Organizer
      2008 Annual Meeting of Japan Societry for Bioscience, Biotechnology, and Agrochemistry
    • Place of Presentation
      Meijo University(Nagogy)
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of recombinant active human renin in SF-9 cells.2007

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of recombinant active human renin in Sf-9 cells.2007

    • Author(s)
      Saori Takahashi, Keishi Hata, Kazuyuki Hori, Toshihiro Sugiyama, Ken-Ichi Kikuchi, Takeshi Gotoh
    • Organizer
      BMB2007
    • Place of Presentation
      Pacifico Yokohama(Yokohama)
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of recombinant human renin in Sf-9 cells2007

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2007-12-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] Renin inhibitor in legumes.2007

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      ICOFF 2007,International Conference of Food Factors for Health Promotion
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2007-11-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Renin inhibitor in legumes.2007

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
    • Organizer
      ICOFF2007, International Conference on Food Factors for Health Promotion
    • Place of Presentation
      Kyoto International Conference Hall(Kyoto)
    • Year and Date
      2007-11-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Renin inhibitor in Iegumes2007

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      ICOFF 2007, International Conference on Food Factors for Health Promotion
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2007-11-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Mature human renin expressed in insect cells.2007

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      5th General Meeting of the International Proteolysis Society
    • Place of Presentation
      University of Patras(Patras,Greece)
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mature human renin expressed in insect cells.2007

    • Author(s)
      Saori Takahashi, Keishi Hata, Ken-Ichi Kikuchi, Takeshi Gotho
    • Organizer
      5th General Meeting of the International Proteolysis Society
    • Place of Presentation
      University of Patras, (Patras, Greece)
    • Year and Date
      2007-10-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mature human renin expressed in insect cells2007

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      5th General Meeting of the International Proteolys is Society
    • Place of Presentation
      パトラ大学(ギリシャ)
    • Year and Date
      2007-10-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 組換え型ヒトレニンを用いた阻害物質スクリーニング系の構築2007

    • Author(s)
      高橋砂織, 他5名
    • Organizer
      食品酵素化学研究会第7回学術講演会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2007-09-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Establishment of renin inhibitor screening method using recombinant enzyme.(japanese)2007

    • Author(s)
      Saori Takahashi, Takayuki Watanabe, Hironobu Ogasawara, Masanori Kumagai, Kazuyuki Hori, Mari Sugawara
    • Organizer
      7th Annual Meeting of Society for Food Enzyme Chemistry
    • Place of Presentation
      Kyoto University(Kyoto)
    • Year and Date
      2007-09-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 食物由来レニン阻害物質について2007

    • Author(s)
      高橋砂織, 他6名
    • Organizer
      食品化学工学会54回大会
    • Place of Presentation
      中村学園大学(福岡市)
    • Year and Date
      2007-09-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Renin inhibitor from foods.(Japanese)2007

    • Author(s)
      Saori Takahashi, Takayuki Watanabe, Hironobu Ogasawara, Masanori Kumagai, Keishi Hata, Kazuyuki Hori, Mari Sugawara
    • Organizer
      54th Annual Meeting of Japanese Society for Food Science and Technlogy
    • Place of Presentation
      Nakamura-Gakuen University(Fukuoka)
    • Year and Date
      2007-09-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 食物由来レニン阻害物質2007

    • Author(s)
      高橋 砂織, 他6名
    • Organizer
      食品科学工学会第54回大会
    • Place of Presentation
      中村学園大学(福岡市)
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Novel assay method for GlcNAc 2-epimerase using N-acylhexosamine oxidase and peroxidase.(Japanese)2007

    • Author(s)
      Saori Takahashi, Kazuyuki Hori
    • Organizer
      21st Chitin and Chitosan Symposium
    • Place of Presentation
      Kobe International Conference Hall(kobe)
    • Year and Date
      2007-08-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] N-アシルヘキソサミン酸化酵素とペルオキシダーゼとの共役系を用いたGlcNAc 2-エピメラーゼの活性測定法について2007

    • Author(s)
      高橋砂織、堀 一之
    • Organizer
      第21回キチン・キトサンシンポジム
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2007-07-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] N-アシルヘキソサミン酸化酵素とペルオキシダーゼとの共役系を用いたGlcNAc2-エピメラーゼの活性測定法について2007

    • Author(s)
      高橋 砂織、堀 一之
    • Organizer
      第21回キチン・キトサンシンポジム
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2007-07-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 味噌及び大豆由来レニン阻害物質について2007

    • Author(s)
      高橋砂織, 他5名
    • Organizer
      2007年度日本農芸化学会大会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Renin inhibitor form miso and soybean.(Japanese)2007

    • Author(s)
      Saori Takahashi, Hironobu Ogasawara, Takayuki Watanabe, Masanori Kumagai, Kaoru Owari, Kazuyuki Hori
    • Organizer
      2007 Annual Meeting of Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Tokyo University of Agriculture(Tokyo)
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Stabilization of GlcNAc 2-epimerase(RnBP) by nucleotides.2006

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      7th International Conference of Europe Chitin Chitosan Society and 10th International Conference of Chitin and Chitosan
    • Place of Presentation
      Montpelier(France)
    • Year and Date
      2006-09-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Stabilization of GlcNAc 2-epimerase(Renin binding protein)by nucleotides.2006

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Toshihiro Sugiyama
    • Organizer
      7th International Conference of the European Chitin Society and 10th International Conference of Chitin and Chitosan
    • Place of Presentation
      Montpelier(France)
    • Year and Date
      2006-09-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] GlcNAc 2-エピメラーゼ(レニン結合タンパク質)とレニンとの相互作用に及ぼすヌクレオチドの役割2006

    • Author(s)
      高橋砂織、堀 一之, 他3名
    • Organizer
      第20回キチン・キトサンシンポジウム
    • Place of Presentation
      福井工業大学(福井市)
    • Year and Date
      2006-08-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of nucleotides on the interaction of renin with GlcNAc 2-epimerase(Renin binding protein).(Japanese)2006

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Kazuyuki Hiwatashi, Hironobu Ogasawara, Toshihiro Sugiyama
    • Organizer
      20th Chitin Chitosan Symposium
    • Place of Presentation
      Fukui Institute of Technology(Fukui)
    • Year and Date
      2006-08-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Role of nucleotides on the formation of a renin binding protein(RnBP) homodimer and an RnBP-renin heterodimer.2006

    • Author(s)
      Saori Takahashi, et. al.
    • Organizer
      20th IUBMB International Congress of Biochemistry and 11th FAOBMB.
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Role of nucleotides on the formation of renin binding protein(RnBP)homodimer and an RnBP-renin heterodimer.2006

    • Author(s)
      Saori Takahashi, Kazuyuki Hori, Toshihiro Sugiyama
    • Organizer
      20th IUBMB and 11th FAOBMB
    • Place of Presentation
      Kyoto International Conference Hall(Kyoto)
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Advances in Chitin Science Volume IX2006

    • Author(s)
      Saori Takahashi, et. al.
    • Total Pages
      6
    • Publisher
      10th I.C.C.C/EUCHIS'06(CD Version)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.arif.pref.akita.jp

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.arif.pref.akita.jp/

    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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