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Molecular ecological study for conservation of endangered species, Pyrus calleryana Decne var. dimorphopheylla (Makino) Koidz. : Genetic analysis on population structure, gene flow and reproductive deterioration

Research Project

Project/Area Number 18580142
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学・森林工学
Research InstitutionGifu University

Principal Investigator

MUKAI Yuzuru  Gifu University, Faculty of Applied Biological Sciences, Professor (80283349)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsPyrus calleryana / endangered species / self-incompatibility / SSR marker / genetic diversity / genetic structure / gene flow / conservation / SSP / (5)遺伝的構造 / SSR / 遺伝的浮動
Research Abstract

In order to obtain genetic information for conservation of Pyrus calleryana Decne var. dimorphopheylla (Makino) Koidz., the endangered species, all of the individuals exist in Japan were sampled and inquired their origin, revealed that total number of individuals is 465 and they distributed only in Aich and Mie Prefecture. Genotypes of them were determined with ten nuclear SSR markers. Genetic diversity was maintained in this species, however, patterns of "isolation by distance" was not clear among all populations containing those affected by serious human impacts, such as, planting and transplanting. In contrast, genetic structure among the native populations was conserved. Phylogenetic tree and Bayesian cluster analysis revealed that the native populations were divided into two clusters, those are Aichi and Mie. Analyzing the nucleotide sequences for cDNAs corresponding to the alleles of the self-incompatible locus revealed eleven different alleles. Using the specific primers designed for each of S-allele, S-genotypes for 247 individuals collected from ten populations were determined and revealed that four to ten S-alleles were maintained in each population. Paternal analysis for seeds collected from four mother trees in the isolated population was performed with nine SSR markers and revealed that pollen parents were widely distributed in the population. Ecological and genetic factors which affect male contribution was analyzed with generalized linear mixed model (GLMM) and revealed that distance from the mother is the most significant factor for male reproductive success. Although self-incompatibility was functioned in the mating event within the population, degree of S-genotype identity between parents did not strongly affect pollen mediated gene dispersal.
These results will provide important information for planning conservation programs of this species.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (11 results)

All 2008 2006

All Journal Article (2 results) Presentation (9 results)

  • [Journal Article] レッドリストの生き物たち 52 マメナシ2008

    • Author(s)
      加藤珠理
    • Journal Title

      森林技術 797

      Pages: 32-33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Species in Red List. 52. Pyrus calleryana Decnevar. dimorphopheylla (Makino) Koidz. (in Japanese)2008

    • Author(s)
      Kato, S.
    • Journal Title

      Sinrin Gijutu 797

      Pages: 32-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 絶滅危惧種マメナシ自生個体群の遺伝的多様性と繁殖能力2008

    • Author(s)
      西岡理絵, 加藤珠理, 今井淳, 向井譲
    • Organizer
      第57回日本森林学会中部支部大会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2008-10-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic diversity and reproductive capacity in the natural populations Pyrus of calleryana2008

    • Author(s)
      Nisioka, R Kato, S. Imai, J.. Mukai, Y
    • Organizer
      57^<th> Meeting of Chubu-branch Japanese Forest Society
    • Place of Presentation
      Gifu University
    • Year and Date
      2008-10-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 絶滅危惧種マメナシにおける遺伝構造の解明2008

    • Author(s)
      今井淳, 加藤珠理, 向井譲
    • Organizer
      第119回日本森林学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic structure of the endangered species, Pyrus calleryana.2008

    • Author(s)
      Imai, J. Kato, S. Mukai, Y
    • Organizer
      119^<th> Meeting of Japanese Forest Society
    • Place of Presentation
      Tokyo University of Agriculture and Technology
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 絶滅危惧種マメナシにおける遺伝構造の解明2008

    • Author(s)
      今井 淳・加藤珠理・向井 譲
    • Organizer
      第119回日本森林学会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 絶滅危惧種マメナシにおける自家不和合性遺伝子の多様性が種子生産に及ぼす影響2008

    • Author(s)
      加藤珠理, 今井淳, 西岡理絵, 向井譲
    • Organizer
      第55回日本生態学会大会
    • Place of Presentation
      九州大学
    • Year and Date
      2008-03-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of genetic diversity at the self-incompatible locus on the seed production in endangered species, Pyrus calleryana.2008

    • Author(s)
      Kato. S. Imai, J. Nisioka, R. Mukai, Y.
    • Organizer
      55th Annual Meeting of Ecological Sciety of Japan
    • Place of Presentation
      Kyushu University
    • Year and Date
      2008-03-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 絶滅危惧種マメナシにおける種子の生産性と自家不和合性2006

    • Author(s)
      加藤珠理, 岩本祐佳, 松岡美帆, 向井譲
    • Organizer
      第53回日本生態学会大会
    • Place of Presentation
      新潟大学
    • Year and Date
      2006-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Seed productivity and self-incompatibility in the endangered species, Pyrus calleryana2006

    • Author(s)
      Kato, S. Iwamoto, Y. Matuoka, M. Mukai, Y.
    • Organizer
      53th Annual Meeting of Ecological Society of Japan
    • Place of Presentation
      Niigata University
    • Year and Date
      2006-03-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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