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Research and development on purification methods for dioxins, environmental ha-manes and indnerated ashes by use of a minnbial preparations and an enzyme preparations prepared from a fungus capable of degradation of dioxins

Research Project

Project/Area Number 18580166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産科学・木質工学
Research InstitutionEhime University

Principal Investigator

TACHIBANA Sanro  Ehime University, Faculty of Agriculture, Professor (10112319)

Co-Investigator(Kenkyū-buntansha) KAZUTAKA Ikuo  Ehime University, Faculty of Agriculture, Associate Professor (50253323)
NINOMIYA Ikuo  Ehime University, Faculty of Agriculture, Professor (80172732)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsPurification / Dioxins / Environmental hormones / White-rot fungi / Microbial preparations / Enzyme preparations / Dioxin-contaminated water / ダイオキシン汚染土壌 / バイオリアクター
Research Abstract

The purification methods for dioxins, environmental hormones, and incinerated ash were tried to develop by use of microbial and enzyme preparations made from a fungus capable of degradation of dioxins, and the following results were obtained.
1. The purification method of dioxin- and environmental hormone-contaminated soils by use of the microbial preparations was developed by investigating the added amount of the preparations and treatment time and so on. Eight four percent of the DDT-contaminated soil could be purified by the method for 30 days with 5% of the preparations. And, 35 to 66% of the dioxins (2,8-DCDD and 2,4,8-TCDF)-contaminated soils were purified by the method for 30 days with 2 to 5% of the preparations. Furthermore, the method for enhancement of growth of the fungus on the preparations in soil was also developed by addition of nutrients.
2. Enzyme preparations were made from a crude enzyme from the fungus by capsulation. The purification methods of the contaminated soil … More s as well as those waters were developed by using the preparations. Twenty seven and 15% of DDT- and dioxin (TCB)-contaminated soils were purified by the method for 30 days, respectively. And, thirty five and 26% of DIM' and TCB-contaminated waters were also purified by using the preparations for 3 days, respectively. Furthermore, the enzyme activity in the preparations could be stabilized by clarification of the stabilizing factors. The preparations could be reused at least two times when the contaminated waters were purified by the method. The evaluation method for degradation capability of the preparations was also developed.
3. The purification method for an incinerated ash was developed by using the microbial preparations with soil having pH buffer capacity and a surfactant enhancing growth of the fungus. About 40% of dioxins (PCDD/Fs) in the ash were purified by the method for 30 days. However, degradation of PCDD/Fs in the ash with the enzyme preparations could not be almost preceded by the inactivation of enzymes for the alkalinity of ash.
4. There was no effect of microorganisms originally existed in the soil and dehydrogenase activity related to the activity of microorganisms in soil on the treatment of soil with the microbial and enzyme preparations. Effect of plant cultivation on the soil treatment was not also recognized. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (13 results)

All 2007 2006

All Journal Article (7 results) Presentation (6 results)

  • [Journal Article] 白色腐朽菌からの微生物製剤作製の試み2006

    • Author(s)
      佐々木 良輝
    • Journal Title

      第51回リグニン討論会講演集

      Pages: 148-149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] ダイオキシン分解菌からの酵素を用いた酵素製剤作製の試み2006

    • Author(s)
      藤井 啓太
    • Journal Title

      第51回リグニン討論会講演集

      Pages: 150-151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Attempts to make a microbial preparation from a white-rot fungus2006

    • Author(s)
      Yoshiteru, Sasaki, Kenta, Furuyama, Kazutaka, Itoh, Sanro, Tachibana
    • Journal Title

      Proceedings of the 51th Lignin Symposium, Sapporo

      Pages: 148-149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Attempts to make anenzyme preparation by crude enzyme from fungus capable of dioxins2006

    • Author(s)
      Keita, Fujii, Sanro, Tachibana, Kouki, Kurita, Satoshi, Somamoto, Kazutaka Itoh
    • Journal Title

      Proceedings of the 51th Lignin Symposium, Sapporo

      Pages: 150-151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 白色腐朽菌および粗酵素を用いた焼却灰中のダイオキシンの分解の試み2006

    • Author(s)
      栗田紘希
    • Journal Title

      第56回日本木材学会大会研究発表要旨集

      Pages: 24-24

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 白色腐朽菌からの微生物製剤作成の試み2006

    • Author(s)
      佐々木良輝
    • Journal Title

      第51回リグニン討論会講演集

      Pages: 148-149

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ダイオキシン分解菌からの酵素を用いた酵素製剤作成の試み2006

    • Author(s)
      藤井啓太
    • Journal Title

      第51回リグニン討論会講演集

      Pages: 150-151

    • Related Report
      2006 Annual Research Report
  • [Presentation] 白色腐朽菌からの酵素製剤を用いた水中のDDT分解の試み2007

    • Author(s)
      杣本 聡
    • Organizer
      第57回日本木材学会大会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 数種の担体を用いた微生物製剤作製の試み2007

    • Author(s)
      古山 健太
    • Organizer
      第57回日本木材学会大会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Trails for degradation of DDT-contaminated waste water by an ezyme Preparations from white-rot fungi2007

    • Author(s)
      Satoshi, Somamoto, Keita, Fijii, Kazutaka, Itoh, Sanro, Tachibana
    • Organizer
      Abstarcs of the 57th Annual Meeting of The Japan Wood Research Society
    • Place of Presentation
      Hiroshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Trials for preparation of microbial preparations by using some carriers2007

    • Author(s)
      Kenta, Furuyama, Yoshiteru, Sasaki, Kazutaka, Itoh, Sanro, Tachibana
    • Organizer
      Abstracts of the 57th Annual Meeting of The Japan Wood Research Society
    • Place of Presentation
      Hiroshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 白色腐朽菌および粗酵素を用いた焼却灰中のダイオキシンの分解の試み2006

    • Author(s)
      栗田 紘希
    • Organizer
      第56回日本木材学会大会
    • Place of Presentation
      秋田大学
    • Year and Date
      2006-08-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Trials for degradation of dioxins in an incinerated ash by crude enzymes from white-rot fungi2006

    • Author(s)
      Kouki, Kurita, Kazutaka, Itoh, Sanro, Tachibana
    • Organizer
      Abstracts of the 56th Annual Meeting of The Japan Wood Research Society
    • Place of Presentation
      Akita
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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