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Analysis on pathogenic mechanism of fish pathogen Edwardsiella tarda

Research Project

Project/Area Number 18580184
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionKyoto Pharmaceutical University (2007)
Hiroshima University (2006)

Principal Investigator

OKUDA Jun  Kyoto Pharmaceutical University, 薬学部, Associate Professor (90334276)

Co-Investigator(Kenkyū-buntansha) NAKAI Toshihiro  Hiroshima University, 生物圏科学研究科, Professor (60164117)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsEdwardsiella tarda / the type III secretion system / antiphagocytosis / Invasion to epithelial cell / pathogenicity / 新規エフェクター / エフェクター / 食菌抵抗性
Research Abstract

(1) Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system
Edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. Resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. We showed that intracellular replication of E. tarda in murine macrophages is dependent on the type III secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic NF-kappaB target genes. The wild-type strain replicates within the phagosomal membrane of macrophages ; whereas the type III mutant does not.
(2) Microarray analysis
Microarray analysis shows the mRNA expression level of NF-kappaB target genes (e.g. pro-inflammatory cytokines and anti-apoptotic genes) in macrophages infected with the wild-type strain were up-regulated compared to macrophages infected with the type III mutant. Up-regulation of anti-apoptotic NF-kappaB target genes is responsible for the an … More ti-apoptotic activity of E. tarda and is required for intracellular replication in murine macrophages
(3) The type III secretion system-dependent repression of NF-kappaB activation to the intracellular growth of Edwardsiella tarda in human epithelial cells
We next showed that the TTSS is also needed for intracellular growth of the bacterium in human epithelial cells (Hep-2). However, different from the previous microarray analyses on murine macrophages, upregulation of the mRNA expression level of NF-kappaB target genes was not detected in the infected Hep-2 cells. The wild-type E tarda, but not its TTSS mutant, actually repressed the tumor necrosis factor alpha-dependent NF-kappaB activation in an NF-kappaB reporter gene assay. These results suggest TTSS-dependent repression of the NF-kappaB activation in Hep-2 cells infected with E. tarda.
Taken together, these results suggest that drug or vaccine specifically targeting the type III secretion system of this bacterium may contribute to decreasing the industrial loss caused by E. tarda infection to cultured fish such as red sea bream and Japanese flounder. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (42 results)

All 2008 2007 2006 2005 Other

All Journal Article (17 results) (of which Peer Reviewed: 9 results) Presentation (25 results)

  • [Journal Article] The type III secretion system-dependent repression of NF-κB activation to the intracellular growth of Edwardsiella tarda in human epithelial cells2008

    • Author(s)
      Okuda, J., M.Kiriyama, E.Yamanoi, T.Nakai.
    • Journal Title

      FEMS Microbiol.Lett. 283

      Pages: 9-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The type III secretion system-dependent repression of NF-κB activation to the intracellular growth of Edwardsiella tarda in human epithelial cells.2008

    • Author(s)
      Okuda, J., M. Kiriyama, E. Yamanoi, T. Nakai.
    • Journal Title

      FEMS Microbiol. Lett. 283

      Pages: 9-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Base changes in the fliC gene of Edwardsiella tarda:possible effects on flagellation and motility2007

    • Author(s)
      Okuda, J., F.Murayama, E.Yamanoi, E.Iwamoto, S.Matsuoka, M.Nishibuchi, T.Nakai.
    • Journal Title

      Dis.Aquat.Organ. 76

      Pages: 113-121

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Base changes in the fliC gene of Edwardsiella tarda : possible effects on flagellation and motility.2007

    • Author(s)
      Okuda, J., F. Murayama, E. Yamanoi, E. Iwamoto, S. Matsuoka, M. Nishibuchi, T. Nakai.
    • Journal Title

      Dis. Aquat. Organ. 76

      Pages: 113-121

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The potent antibacterial activity of sitafloxacin against fluoroquinolone-resistant clinical isolates ofVibrio cholerae O12007

    • Author(s)
      Okuda, J., T.Ramamurthy, and S.Yamasaki
    • Journal Title

      Microbiol.Immunol 51

      Pages: 467-469

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibacterial activity of ciprofloxacin against clinical strains of Vibrio cholerae O139 recently isolated from India2007

    • Author(s)
      Okuda, J., T.Ramamurthy, and S.Yamasaki
    • Journal Title

      Yakugaku Zasshi 127

      Pages: 903-904

    • NAID

      110006273936

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Base changes in the fliC gene of Edwardsiell atarda:possible effects on flagellation and motility2007

    • Author(s)
      Okuda, J., F.Murayama, E.Yamanoi, E.Ywamoto, S.Matsuoka, M.Nishibuchi and T.Nakai
    • Journal Title

      Dis Aquat.Organ 76

      Pages: 113-121

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of bacteria from the copepod Pseudocaligus fugu ectoparasitic on the panther puffer Takifugu pardalis with the emphasis on TTX2007

    • Author(s)
      Venmathi Maran B.A., E.Iwamoto, J.Okuda, S.Matsuda, S.Taniyama, Y.Shida, M.Asakawa, S.Ohtsuka, T.Nakai and G.A.Boxshall
    • Journal Title

      Toxicon 50

      Pages: 779-790

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-κB target genes protecting the macrophage from staurosporine-induced apoptosis2006

    • Author(s)
      Okuda, J., Y.Arikawa, E., Y.Takeuchi, M.M.Mahmoud, E.Suzaki, K.Kataoka, T.Suzuki, Y.Okinaka, T.Nakai.
    • Journal Title

      Microb.Pathog. 41

      Pages: 226-240

    • NAID

      120000878113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Sodium choloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells2006

    • Author(s)
      Mahmoud, M.M., J.Okuda, T.Nakai
    • Journal Title

      Fish Pathology 41

      Pages: 165-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Induction of hemagglutinating activity of Edwardsiella tarda by sodium chloride2006

    • Author(s)
      Yasunobu H., Y.Arikawa, K.Furutsuka-Uozumi, M.Dombo, T.Iida, M.M.Mahmoud, J.Okuda, T.Nakai.
    • Journal Title

      Fish Pathology 41

      Pages: 29-34

    • NAID

      10020355727

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-κB target genes protecting the macrophage from staurosporine-induced apoptosis.2006

    • Author(s)
      Okuda, J., Y. Arikawa, Y. Takeuchi, M. M. Mahmoud, E. Suzaki, K. Kataoka, T. Suzuki, Y. Okinaka, T. Nakai.
    • Journal Title

      Microb. Pathog. 41

      Pages: 226-240

    • NAID

      120000878113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Induction of hemagglutinating activity of Edwardsiella tarda by sodium chloride.2006

    • Author(s)
      Yasunobu H., Y. Arikawa, K. Furutsuka-Uozumi, M. Dombo, T. Iida, M. M. Mahmoud, J. Okuda, N. Nakai.
    • Journal Title

      Fish Pathology 41

      Pages: 29-34

    • NAID

      10020355727

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sodium choloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells.2006

    • Author(s)
      Mahmoud, M. M., J. Okuda, T. Nakai.
    • Journal Title

      Fish Pathology 41

      Pages: 165-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-kB target genes protecting the macrophage from staurosporine-induced apoptosis.2006

    • Author(s)
      Okuda J.
    • Journal Title

      Microbial Pathogenesis 41

      Pages: 226-240

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sodium choloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells.2006

    • Author(s)
      Mahmoud M. M.
    • Journal Title

      Fish Pathology 41

      Pages: 165-170

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Base changes in the fliC gene of Edwardsiella tarda : possible effects on flagellation and motility.

    • Author(s)
      Okuda J.
    • Journal Title

      Diseases of Aquatic Organisms In press

    • Related Report
      2006 Annual Research Report
  • [Presentation] 人畜魚共通病原細菌Edwardsiella tardaの新規III型エフェクターの機能解析2008

    • Author(s)
      奥田 潤、後藤 直正
    • Organizer
      平成20年度日本細菌学会総会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of invasion mechanism of Edwardsiella tarda to epithelial cells-I[association with the type III secretion system].2008

    • Author(s)
      Okuda, J., N. Gotoh.
    • Organizer
      日本細菌学会
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Edwardsiella tardaの上皮細胞への侵入増殖メカニズムの解析-I[III型分泌装置の関与]2007

    • Author(s)
      奥田 潤、桐山 真、中井 敏博
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Edwardsiella tardaのマクロファージ食菌抵抗性メカニズムの解析-V[エフェクター候補遺伝子と病原性との関係]2007

    • Author(s)
      竹内 佑介、奥田 潤、中井 敏博
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Edwardsiella tardaのマクロファージ食菌抵抗性メカニズムの解析-VI[宿主特異性と食菌抵抗性との関係]2007

    • Author(s)
      桐山 真、奥田 潤、中井 敏博、山下 浩史
    • Organizer
      平成19年度日本魚病学会大会
    • Place of Presentation
      北海道
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sodium chloride-promoted adhesion of Edwardsiella tarda to goldfish intestine2007

    • Author(s)
      Mahmoud, M.M.、奥田 潤、中井 敏博
    • Organizer
      平成19年度日本魚病学会大会
    • Place of Presentation
      北海道
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of invasion mechanism of Edwardsiella tarda to epithelial cells-I[association with the type III secretion system].2007

    • Author(s)
      Okuda, J., M. Kiriyama, T. Nakai.
    • Organizer
      日本水産学会
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of antiphagocytotic mechanism of Edwardsiella tarda-V[virulence of candidates of the E. tarda type III effectors].2007

    • Author(s)
      Takeuchi, Y., J. Okuda, T. Nakai.
    • Organizer
      日本水産学会
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of antiphagocytotic mechanism of Edwardsiella tarda-VI[relationship between host specificity and antipagocytotic effect].2007

    • Author(s)
      Kiriyama, M., J. Okuda, T. Nakai, H. Yamashita.
    • Organizer
      日本魚病学会
    • Place of Presentation
      Hokkaido
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sodium chloride-promoted adhesion of Edwardsiella tarda to goldfish intestine.2007

    • Author(s)
      Mahmoud, M. M., J. Okuda, T. Nakai.
    • Organizer
      日本魚病学会
    • Place of Presentation
      Hokkaido
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Edwardsiella tarda の上皮細胞への侵入増殖メカニズムの解析-I[IIII型分泌装置の関与]2007

    • Author(s)
      奥田 潤、桐山 真、中井 敏博
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] Edwardsiella tarda のマクロファージ食菌抵抗性メカニズムの解析-V[エフェクター候補遺伝子と病原性との関係]2007

    • Author(s)
      竹内 佑介、奥田 潤、中井 敏博
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] Edwardsiella tardaのマクロファージ食菌抵抗性メカニズムの解析-II[抗アポトーシス活性の誘導]2006

    • Author(s)
      奥田 潤、有川 陽子、竹内 佑介、冲中 泰、中井 敏博、洲崎 悦子、片岡 勝子
    • Organizer
      平成18年度日本水産学会
    • Place of Presentation
      高知
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Edwardsiella tardaのマクロファージ食菌抵抗性メカニズムの解析-III[III型分泌装置のトランスローコンの同定]2006

    • Author(s)
      桐山 真、奥田 潤、竹内 佑介、中井 敏博
    • Organizer
      平成18年度日本水産学会
    • Place of Presentation
      高知
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation and characterization of bacteria associated with the copepod Pseudocalig us fugu parasitic on panther puffer Takifugu pardalis2006

    • Author(s)
      Venmathi Maran, B.A., E.Iwamoto, J.Okuda, I.Otsuka, T.Nakai.
    • Organizer
      平成18年度日本水産学会
    • Place of Presentation
      高知
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Edwardsiella tardaのマクロファージ食菌抵抗性メカニズムの解析-IV[III型分泌装置のエフェクター候補]2006

    • Author(s)
      竹内 佑介、奥田 潤、中井 敏博
    • Organizer
      平成18年度日本魚病学会大会
    • Place of Presentation
      長崎
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 培地の塩分濃度とEdwardsiella tardaの細胞付着性2006

    • Author(s)
      Mahmoud, M.M.、奥田 潤、中井 敏博
    • Organizer
      平成18年度日本魚病学会大会
    • Place of Presentation
      長崎
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 非運動性Edwardsiella tardaのfliC遺伝子の変異2006

    • Author(s)
      奥田 潤、村山 史康、山野井 英介、岩本 恵美、中井 敏博
    • Organizer
      平成18年度日本魚病学会大会
    • Place of Presentation
      長崎
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of antiphagocytotic mechanism of Edwardsiella tarda-II[induction of antiapoptotic activity].2006

    • Author(s)
      Okuda, J., Y. Arikawa, Y. Takeuchi, Y. Okinaka, T. Nakai, E. Suzaki, K. Kataoka.
    • Organizer
      日本水産学会
    • Place of Presentation
      Kochi
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of antiphagocytotic mechanism of Edwardsiella tarda-III[identification of the type III translocons].2006

    • Author(s)
      Kiriyama, M., J. Okuda, Y. Takeuchi, T. Nakai
    • Organizer
      日本水産学会
    • Place of Presentation
      Kochi
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Isolation and characterization of bacteria associated with the copepod Pseudocaligus fugu parasitic on panther puffer Takifugu pardalis.2006

    • Author(s)
      Venmathi Maran, B. A., E. Iwamoto, J. Okuda, I. Otsuka, T. Nakai.
    • Organizer
      日本水産学会
    • Place of Presentation
      Kochi
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of antiphagocytotic mechanism of Edwardsiella tarda-IV[candidates of the E. tarda type III effector].2006

    • Author(s)
      Takeuchi, Y., J. Okuda, T. Nakai.
    • Organizer
      日本魚病学会
    • Place of Presentation
      Nagasaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Relationship between salt concentration of the culture medium and adherence ability of Edwardsiella tarda to host cells.2006

    • Author(s)
      Mahmoud, M. M., J. Okuda, T. Nakai.
    • Organizer
      日本魚病学会
    • Place of Presentation
      Nagasaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Base changes in the fliC gene of Edwardsiella tarda : possible effects on flagellation and motility.2006

    • Author(s)
      Okuda, J., F. Murayama, E. Yamanoi, E. Iwamoto, T. Nakai.
    • Organizer
      日本魚病学会
    • Place of Presentation
      Nagasaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of antiphagocytotic mechanism of Edwardsiella tarda on macrophages.2005

    • Author(s)
      Okuda, J., Y. Takeuchi, E. Suzaki, K. Kataoka, T. Nakai.
    • Organizer
      日本魚病学会
    • Place of Presentation
      Mie
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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